Source 1primary paper2017ACS Synthetic Biology
Toolkit/Opto-T7RNAP
Opto-T7RNAP
Also known as: blue light-responsive T7 RNA polymerases, Opto-T7RNAPs
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Opto-T7RNAP is an engineered blue light-inducible T7 RNA polymerase transcription system developed in Escherichia coli. It enables light-controlled transcription with spatiotemporal precision and is intended to function independently of endogenous cellular regulation.
Usefulness & Problems
Why this is useful
This tool is useful for externally controlling transcription with blue light while minimizing coupling to native regulatory networks. Its rapid dark-state recovery and large inducible range support precise temporal modulation of gene expression.
Source:
we present our engineered blue light-responsive T7 RNA polymerases (Opto-T7RNAPs)
Problem solved
Opto-T7RNAP addresses the need for a transcription system that can be activated by light with high dynamic range, low basal activity, and minute-scale reversibility after light removal. It also addresses the challenge of implementing gene control that is orthogonal to endogenous cellular regulation.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
recombinationtranscriptionInput: Light
Implementation Constraints
The system is based on engineered T7 RNA polymerase and uses blue light as the input modality. Available evidence supports implementation in Escherichia coli, but does not provide specific details on expression constructs, cofactors, wavelengths, or delivery strategies.
The supplied evidence indicates development and testing in Escherichia coli, with extension to other bacteria, yeast, or mammalian cells described only as a prospective possibility. The evidence provided does not specify construct architecture, illumination parameters, or validation for recombination control.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successBacteriaapplication demoEscherichia coli
Inferred from claim c2 during normalization. Opto-T7RNAP shows low leakiness in the dark state, high expression strength under blue light induction, and an inducible range greater than 300-fold. Derived from claim c2. Quoted text: show properties such as low leakiness of gene expression in the dark state, high expression strength when induced with blue light, and an inducible range of more than 300-fold
Source:
inducible range300 fold(>)
successBacteriaapplication demoEscherichia coli
Inferred from claim c4 during normalization. Opto-T7RNAP regulators were developed and tested in Escherichia coli. Derived from claim c4. Quoted text: The regulators ... were developed and tested in the bacterium Escherichia coli
Source:
Supporting Sources
Ranked Claims
An optimized Opto-T7RNAP variant returns to the inactive dark state within minutes after blue light is turned off, enabling precise dynamic control of gene expression.
we created a variant that returns to the inactive dark state within minutes once the blue light is turned off
return to inactive dark state time within minutes
Opto-T7RNAP shows low leakiness in the dark state, high expression strength under blue light induction, and an inducible range greater than 300-fold.
show properties such as low leakiness of gene expression in the dark state, high expression strength when induced with blue light, and an inducible range of more than 300-fold
inducible range 300 fold
Opto-T7RNAP may be extendable with minor alterations to other bacterial species and to eukaryotes such as mammalian cells and yeast.
Opto-T7RNAP, with minor alterations, should be extendable to other bacterial species as well as eukaryotes such as mammalian cells and yeast
Opto-T7RNAP regulators were developed and tested in Escherichia coli.
The regulators ... were developed and tested in the bacterium Escherichia coli
Opto-T7RNAP is a blue light-inducible transcription system based on engineered T7 RNA polymerase that is intended to operate independently from cellular regulation.
we present our engineered blue light-responsive T7 RNA polymerases (Opto-T7RNAPs)
Approval Evidence
1 source5 linked approval claimsfirst-pass slug opto-t7rnap
Here, we present our engineered blue light-responsive T7 RNA polymerases (Opto-T7RNAPs)
Source:
dynamic controlsupports
An optimized Opto-T7RNAP variant returns to the inactive dark state within minutes after blue light is turned off, enabling precise dynamic control of gene expression.
we created a variant that returns to the inactive dark state within minutes once the blue light is turned off
Source:
performancesupports
Opto-T7RNAP shows low leakiness in the dark state, high expression strength under blue light induction, and an inducible range greater than 300-fold.
show properties such as low leakiness of gene expression in the dark state, high expression strength when induced with blue light, and an inducible range of more than 300-fold
Source:
prospective scopeneutral
Opto-T7RNAP may be extendable with minor alterations to other bacterial species and to eukaryotes such as mammalian cells and yeast.
Opto-T7RNAP, with minor alterations, should be extendable to other bacterial species as well as eukaryotes such as mammalian cells and yeast
Source:
tested in systemsupports
Opto-T7RNAP regulators were developed and tested in Escherichia coli.
The regulators ... were developed and tested in the bacterium Escherichia coli
Source:
tool propertysupports
Opto-T7RNAP is a blue light-inducible transcription system based on engineered T7 RNA polymerase that is intended to operate independently from cellular regulation.
we present our engineered blue light-responsive T7 RNA polymerases (Opto-T7RNAPs)
Source:
Comparisons
Source-backed strengths
Reported Opto-T7RNAP variants show low leakiness in the dark state, high expression strength under blue light, and an inducible range greater than 300-fold. An optimized variant returns to the inactive dark state within minutes after blue light is turned off, supporting dynamic control of expression.
Source:
show properties such as low leakiness of gene expression in the dark state, high expression strength when induced with blue light, and an inducible range of more than 300-fold
Ranked Citations
- 1.