Source 1primary paper2021
Toolkit/OptoAssay
OptoAssay
Also known as: light-controlled assay
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
OptoAssay is a light-controlled dynamic bioassay that integrates optogenetic switches to regulate assay behavior. It was introduced as the first light-controlled assay for wash- and pump-free point-of-care diagnostics and enables wavelength-dependent, reversible control of assay components.
Usefulness & Problems
Why this is useful
OptoAssay is useful as a diagnostic assay format that replaces conventional fluid-handling steps with light-controlled assay dynamics. The reported concept is intended to support on-site analysis and may reduce dependence on external flow-control systems and separate signal readout devices when combined with smartphones.
Problem solved
OptoAssay addresses the need for wash- and pump-free point-of-care diagnostics. It specifically targets the challenge of controlling assay component movement and assay progression without external pumping or washing hardware.
Problem links
Need a controllable or interpretable biological readout
DerivedOptoAssay is a light-controlled dynamic bioassay that integrates optogenetic switches to regulate assay behavior. It was introduced as the first light-controlled assay for wash- and pump-free point-of-care diagnostics and enables wavelength-dependent, reversible control of assay components.
Need precise spatiotemporal control with light input
DerivedOptoAssay is a light-controlled dynamic bioassay that integrates optogenetic switches to regulate assay behavior. It was introduced as the first light-controlled assay for wash- and pump-free point-of-care diagnostics and enables wavelength-dependent, reversible control of assay components.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
bidirectional movement of assay componentslight-dependent reversible binding interactionswavelength-controlled switchingTechniques
Functional AssayTarget processes
diagnosticInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
Implementation relies on integrating optogenetic switches into the assay architecture and controlling the system with light in a wavelength-dependent manner. The provided evidence does not specify the optogenetic proteins, illumination wavelengths, construct design, cofactors, or substrate materials beyond noting efficacy on various substrates.
The supplied evidence does not provide quantitative performance metrics, analyte scope, sensitivity, specificity, or direct comparisons to established diagnostic assays. Smartphone integration and resource-limited deployment are presented as proposed future applications rather than independently validated implementations in the provided evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2024Science Advances
Ranked Claims
OptoAssay showed efficacy on various substrates and provides a dynamic bioassay format.
Demonstrating exceptional versatility, the OptoAssay showcases its efficacy on various substrates, delivering a dynamic bioassay format
OptoAssay showed efficacy on various substrates and provides a dynamic bioassay format.
Demonstrating exceptional versatility, the OptoAssay showcases its efficacy on various substrates, delivering a dynamic bioassay format
OptoAssay showed efficacy on various substrates and provides a dynamic bioassay format.
Demonstrating exceptional versatility, the OptoAssay showcases its efficacy on various substrates, delivering a dynamic bioassay format
OptoAssay showed efficacy on various substrates and provides a dynamic bioassay format.
Demonstrating exceptional versatility, the OptoAssay showcases its efficacy on various substrates, delivering a dynamic bioassay format
OptoAssay showed efficacy on various substrates and provides a dynamic bioassay format.
Demonstrating exceptional versatility, the OptoAssay showcases its efficacy on various substrates, delivering a dynamic bioassay format
OptoAssay showed efficacy on various substrates and provides a dynamic bioassay format.
Demonstrating exceptional versatility, the OptoAssay showcases its efficacy on various substrates, delivering a dynamic bioassay format
OptoAssay showed efficacy on various substrates and provides a dynamic bioassay format.
Demonstrating exceptional versatility, the OptoAssay showcases its efficacy on various substrates, delivering a dynamic bioassay format
The authors propose that OptoAssays combined with smartphones could remove the need for external flow control systems and signal readout devices for on-site analysis in resource-limited settings.
In the future, combined with smartphones, OptoAssays could obviate the need for external flow control systems such as pumps or valves and signal readout devices, enabling on-site analysis in resource-limited settings
The authors propose that OptoAssays combined with smartphones could remove the need for external flow control systems and signal readout devices for on-site analysis in resource-limited settings.
In the future, combined with smartphones, OptoAssays could obviate the need for external flow control systems such as pumps or valves and signal readout devices, enabling on-site analysis in resource-limited settings
The authors propose that OptoAssays combined with smartphones could remove the need for external flow control systems and signal readout devices for on-site analysis in resource-limited settings.
In the future, combined with smartphones, OptoAssays could obviate the need for external flow control systems such as pumps or valves and signal readout devices, enabling on-site analysis in resource-limited settings
The authors propose that OptoAssays combined with smartphones could remove the need for external flow control systems and signal readout devices for on-site analysis in resource-limited settings.
In the future, combined with smartphones, OptoAssays could obviate the need for external flow control systems such as pumps or valves and signal readout devices, enabling on-site analysis in resource-limited settings
The authors propose that OptoAssays combined with smartphones could remove the need for external flow control systems and signal readout devices for on-site analysis in resource-limited settings.
In the future, combined with smartphones, OptoAssays could obviate the need for external flow control systems such as pumps or valves and signal readout devices, enabling on-site analysis in resource-limited settings
The authors propose that OptoAssays combined with smartphones could remove the need for external flow control systems and signal readout devices for on-site analysis in resource-limited settings.
In the future, combined with smartphones, OptoAssays could obviate the need for external flow control systems such as pumps or valves and signal readout devices, enabling on-site analysis in resource-limited settings
The authors propose that OptoAssays combined with smartphones could remove the need for external flow control systems and signal readout devices for on-site analysis in resource-limited settings.
In the future, combined with smartphones, OptoAssays could obviate the need for external flow control systems such as pumps or valves and signal readout devices, enabling on-site analysis in resource-limited settings
The paper introduces OptoAssay as a light-controlled assay for wash- and pump-free point-of-care diagnostics.
we introduce a light-controlled assay, OptoAssay, toward wash- and pump-free point-of-care diagnostics
The paper introduces OptoAssay as a light-controlled assay for wash- and pump-free point-of-care diagnostics.
we introduce a light-controlled assay, OptoAssay, toward wash- and pump-free point-of-care diagnostics
The paper introduces OptoAssay as a light-controlled assay for wash- and pump-free point-of-care diagnostics.
we introduce a light-controlled assay, OptoAssay, toward wash- and pump-free point-of-care diagnostics
The paper introduces OptoAssay as a light-controlled assay for wash- and pump-free point-of-care diagnostics.
we introduce a light-controlled assay, OptoAssay, toward wash- and pump-free point-of-care diagnostics
The paper introduces OptoAssay as a light-controlled assay for wash- and pump-free point-of-care diagnostics.
we introduce a light-controlled assay, OptoAssay, toward wash- and pump-free point-of-care diagnostics
The paper introduces OptoAssay as a light-controlled assay for wash- and pump-free point-of-care diagnostics.
we introduce a light-controlled assay, OptoAssay, toward wash- and pump-free point-of-care diagnostics
The paper introduces OptoAssay as a light-controlled assay for wash- and pump-free point-of-care diagnostics.
we introduce a light-controlled assay, OptoAssay, toward wash- and pump-free point-of-care diagnostics
OptoAssays use light-dependent and reversible interactions of optogenetic switches to enable bidirectional movement of assay components by changing light wavelength.
with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bidirectional movement of assay components, only by changing the wavelength of light
OptoAssays use light-dependent and reversible interactions of optogenetic switches to enable bidirectional movement of assay components by changing light wavelength.
with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bidirectional movement of assay components, only by changing the wavelength of light
OptoAssays use light-dependent and reversible interactions of optogenetic switches to enable bidirectional movement of assay components by changing light wavelength.
with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bidirectional movement of assay components, only by changing the wavelength of light
OptoAssays use light-dependent and reversible interactions of optogenetic switches to enable bidirectional movement of assay components by changing light wavelength.
with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bidirectional movement of assay components, only by changing the wavelength of light
OptoAssays use light-dependent and reversible interactions of optogenetic switches to enable bidirectional movement of assay components by changing light wavelength.
with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bidirectional movement of assay components, only by changing the wavelength of light
OptoAssays use light-dependent and reversible interactions of optogenetic switches to enable bidirectional movement of assay components by changing light wavelength.
with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bidirectional movement of assay components, only by changing the wavelength of light
OptoAssays use light-dependent and reversible interactions of optogenetic switches to enable bidirectional movement of assay components by changing light wavelength.
with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bidirectional movement of assay components, only by changing the wavelength of light
In calibration of a competitive model assay, OptoAssay achieved a limit of detection of 8 pg ml^-1, described as superior to conventional ELISA tests.
resulting in a superior limit of detection of 8 pg ml-1, which is beyond those of conventional ELISA tests
limit of detection 8 pg ml^-1
In calibration of a competitive model assay, OptoAssay achieved a limit of detection of 8 pg ml^-1, described as superior to conventional ELISA tests.
resulting in a superior limit of detection of 8 pg ml-1, which is beyond those of conventional ELISA tests
limit of detection 8 pg ml^-1
In calibration of a competitive model assay, OptoAssay achieved a limit of detection of 8 pg ml^-1, described as superior to conventional ELISA tests.
resulting in a superior limit of detection of 8 pg ml-1, which is beyond those of conventional ELISA tests
limit of detection 8 pg ml^-1
In calibration of a competitive model assay, OptoAssay achieved a limit of detection of 8 pg ml^-1, described as superior to conventional ELISA tests.
resulting in a superior limit of detection of 8 pg ml-1, which is beyond those of conventional ELISA tests
limit of detection 8 pg ml^-1
In calibration of a competitive model assay, OptoAssay achieved a limit of detection of 8 pg ml^-1, described as superior to conventional ELISA tests.
resulting in a superior limit of detection of 8 pg ml-1, which is beyond those of conventional ELISA tests
limit of detection 8 pg ml^-1
In calibration of a competitive model assay, OptoAssay achieved a limit of detection of 8 pg ml^-1, described as superior to conventional ELISA tests.
resulting in a superior limit of detection of 8 pg ml-1, which is beyond those of conventional ELISA tests
limit of detection 8 pg ml^-1
In calibration of a competitive model assay, OptoAssay achieved a limit of detection of 8 pg ml^-1, described as superior to conventional ELISA tests.
resulting in a superior limit of detection of 8 pg ml-1, which is beyond those of conventional ELISA tests
limit of detection 8 pg ml^-1
When combined with smartphones, OptoAssays obviate the need for external flow control systems such as pumps or valves and signal readout devices.
Combined with smartphones, OptoAssays obviate the need for external flow control systems like pumps or valves and signal readout devices.
When combined with smartphones, OptoAssays obviate the need for external flow control systems such as pumps or valves and signal readout devices.
Combined with smartphones, OptoAssays obviate the need for external flow control systems like pumps or valves and signal readout devices.
When combined with smartphones, OptoAssays obviate the need for external flow control systems such as pumps or valves and signal readout devices.
Combined with smartphones, OptoAssays obviate the need for external flow control systems like pumps or valves and signal readout devices.
When combined with smartphones, OptoAssays obviate the need for external flow control systems such as pumps or valves and signal readout devices.
Combined with smartphones, OptoAssays obviate the need for external flow control systems like pumps or valves and signal readout devices.
When combined with smartphones, OptoAssays obviate the need for external flow control systems such as pumps or valves and signal readout devices.
Combined with smartphones, OptoAssays obviate the need for external flow control systems like pumps or valves and signal readout devices.
When combined with smartphones, OptoAssays obviate the need for external flow control systems such as pumps or valves and signal readout devices.
Combined with smartphones, OptoAssays obviate the need for external flow control systems like pumps or valves and signal readout devices.
When combined with smartphones, OptoAssays obviate the need for external flow control systems such as pumps or valves and signal readout devices.
Combined with smartphones, OptoAssays obviate the need for external flow control systems like pumps or valves and signal readout devices.
This paper introduces OptoAssay as a light-controlled assay for wash- and pump-free point-of-care diagnostics.
we introduce the first light-controlled assay, the OptoAssay, towards wash- and pump-free point-of-care diagnostics
This paper introduces OptoAssay as a light-controlled assay for wash- and pump-free point-of-care diagnostics.
we introduce the first light-controlled assay, the OptoAssay, towards wash- and pump-free point-of-care diagnostics
This paper introduces OptoAssay as a light-controlled assay for wash- and pump-free point-of-care diagnostics.
we introduce the first light-controlled assay, the OptoAssay, towards wash- and pump-free point-of-care diagnostics
This paper introduces OptoAssay as a light-controlled assay for wash- and pump-free point-of-care diagnostics.
we introduce the first light-controlled assay, the OptoAssay, towards wash- and pump-free point-of-care diagnostics
This paper introduces OptoAssay as a light-controlled assay for wash- and pump-free point-of-care diagnostics.
we introduce the first light-controlled assay, the OptoAssay, towards wash- and pump-free point-of-care diagnostics
This paper introduces OptoAssay as a light-controlled assay for wash- and pump-free point-of-care diagnostics.
we introduce the first light-controlled assay, the OptoAssay, towards wash- and pump-free point-of-care diagnostics
This paper introduces OptoAssay as a light-controlled assay for wash- and pump-free point-of-care diagnostics.
we introduce the first light-controlled assay, the OptoAssay, towards wash- and pump-free point-of-care diagnostics
OptoAssays use light-dependent and reversible interaction of optogenetic switches to enable bi-directional movement of assay components by changing the wavelength of light.
Extending the capabilities of standard bioassays with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bi-directional movement of assay components, only by changing the wavelength of light.
OptoAssays use light-dependent and reversible interaction of optogenetic switches to enable bi-directional movement of assay components by changing the wavelength of light.
Extending the capabilities of standard bioassays with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bi-directional movement of assay components, only by changing the wavelength of light.
OptoAssays use light-dependent and reversible interaction of optogenetic switches to enable bi-directional movement of assay components by changing the wavelength of light.
Extending the capabilities of standard bioassays with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bi-directional movement of assay components, only by changing the wavelength of light.
OptoAssays use light-dependent and reversible interaction of optogenetic switches to enable bi-directional movement of assay components by changing the wavelength of light.
Extending the capabilities of standard bioassays with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bi-directional movement of assay components, only by changing the wavelength of light.
OptoAssays use light-dependent and reversible interaction of optogenetic switches to enable bi-directional movement of assay components by changing the wavelength of light.
Extending the capabilities of standard bioassays with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bi-directional movement of assay components, only by changing the wavelength of light.
OptoAssays use light-dependent and reversible interaction of optogenetic switches to enable bi-directional movement of assay components by changing the wavelength of light.
Extending the capabilities of standard bioassays with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bi-directional movement of assay components, only by changing the wavelength of light.
OptoAssays use light-dependent and reversible interaction of optogenetic switches to enable bi-directional movement of assay components by changing the wavelength of light.
Extending the capabilities of standard bioassays with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bi-directional movement of assay components, only by changing the wavelength of light.
Approval Evidence
2 sources8 linked approval claimsfirst-pass slug optoassay
we introduce a light-controlled assay, OptoAssay
Source:
we introduce the first light-controlled assay, the OptoAssay
Source:
applicabilitysupports
OptoAssay showed efficacy on various substrates and provides a dynamic bioassay format.
Demonstrating exceptional versatility, the OptoAssay showcases its efficacy on various substrates, delivering a dynamic bioassay format
Source:
future applicationsupports
The authors propose that OptoAssays combined with smartphones could remove the need for external flow control systems and signal readout devices for on-site analysis in resource-limited settings.
In the future, combined with smartphones, OptoAssays could obviate the need for external flow control systems such as pumps or valves and signal readout devices, enabling on-site analysis in resource-limited settings
Source:
introductionsupports
The paper introduces OptoAssay as a light-controlled assay for wash- and pump-free point-of-care diagnostics.
we introduce a light-controlled assay, OptoAssay, toward wash- and pump-free point-of-care diagnostics
Source:
mechanismsupports
OptoAssays use light-dependent and reversible interactions of optogenetic switches to enable bidirectional movement of assay components by changing light wavelength.
with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bidirectional movement of assay components, only by changing the wavelength of light
Source:
performancesupports
In calibration of a competitive model assay, OptoAssay achieved a limit of detection of 8 pg ml^-1, described as superior to conventional ELISA tests.
resulting in a superior limit of detection of 8 pg ml-1, which is beyond those of conventional ELISA tests
Source:
device integrationsupports
When combined with smartphones, OptoAssays obviate the need for external flow control systems such as pumps or valves and signal readout devices.
Combined with smartphones, OptoAssays obviate the need for external flow control systems like pumps or valves and signal readout devices.
Source:
introduction of methodsupports
This paper introduces OptoAssay as a light-controlled assay for wash- and pump-free point-of-care diagnostics.
we introduce the first light-controlled assay, the OptoAssay, towards wash- and pump-free point-of-care diagnostics
Source:
mechanismsupports
OptoAssays use light-dependent and reversible interaction of optogenetic switches to enable bi-directional movement of assay components by changing the wavelength of light.
Extending the capabilities of standard bioassays with light-dependent and reversible interaction of optogenetic switches, OptoAssays enable a bi-directional movement of assay components, only by changing the wavelength of light.
Source:
Comparisons
Source-backed strengths
The available evidence indicates that OptoAssay provides a dynamic bioassay format and showed efficacy on various substrates. Its core advantage is reversible, wavelength-controlled operation of assay behavior through optogenetic switching, which supports bidirectional movement of assay components.
Source:
resulting in a superior limit of detection of 8 pg ml-1, which is beyond those of conventional ELISA tests
Compared with qRT-PCR
OptoAssay and qRT-PCR address a similar problem space because they share diagnostic.
Shared frame: same top-level item type; shared target processes: diagnostic; same primary input modality: light
Relative tradeoffs: appears more independently replicated.
Compared with Raman spectroscopy
OptoAssay and Raman spectroscopy address a similar problem space because they share diagnostic.
Shared frame: same top-level item type; shared target processes: diagnostic; same primary input modality: light
Relative tradeoffs: appears more independently replicated.
Compared with spatial transcriptomics
OptoAssay and spatial transcriptomics address a similar problem space because they share diagnostic.
Shared frame: same top-level item type; shared target processes: diagnostic; same primary input modality: light
Ranked Citations
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