Source 1primary paper2023Communications Physics
Toolkit/optogenetic control of contractility
optogenetic control of contractility
Also known as: optogenetic activation or inhibition of contractility, spatially control contraction
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Optogenetic control of contractility is a light-based engineering method proposed to spatially modulate cellular contractility and thereby influence cell migration behavior. In a one-dimensional active gel model, optogenetic activation or inhibition of contractility is predicted to switch cells between sessile and motile states at realistic parameter values.
Usefulness & Problems
Why this is useful
This approach is useful as a conceptual strategy for controlling migration state by directly perturbing contractility with light. The cited work specifically positions spatial control of contraction as a way to influence cell migration in systems where adhesion and contractility govern state behavior.
Problem solved
It addresses the problem of how to reversibly switch contractile cells between sessile and motile states using a spatially precise external input. The model also suggests that targeting contractility may be more effective than relying on actin polymerization alone, which switched migration direction only at high strength in the comparison described.
Problem links
Need precise spatiotemporal control with light input
DerivedOptogenetic control of contractility is a light-based engineering approach proposed to spatially modulate cellular contraction and thereby influence cell migration state. In a one-dimensional active gel model, optogenetic activation or inhibition of contractility is predicted to switch cells between sessile and motile states at realistic parameter values.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Mechanisms
bistabilityoptogenetic activationoptogenetic activationoptogenetic inhibitionoptogenetic inhibitionspatial control of contractilityspatial control of contractilitystate switching between sessile and motile behaviorsstate switching between sessile and motile behaviorsTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: builder
The available evidence indicates only that light is the input modality and that spatial control of contraction is the intended mode of actuation. Practical details such as construct design, cofactors, expression system, illumination parameters, and delivery strategy are not specified in the supplied evidence.
The evidence provided is theoretical and comes from a one-dimensional active gel model rather than direct experimental validation of a specific molecular construct. No specific optogenetic actuator, wavelength, protein target, cell type, or in vivo performance data are given in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Actin polymerization alone can switch migration direction only at high strength.
show that actin polymerization alone can affect a switch in direction only at high strength
Actin polymerization alone can switch migration direction only at high strength.
show that actin polymerization alone can affect a switch in direction only at high strength
Actin polymerization alone can switch migration direction only at high strength.
show that actin polymerization alone can affect a switch in direction only at high strength
Actin polymerization alone can switch migration direction only at high strength.
show that actin polymerization alone can affect a switch in direction only at high strength
Actin polymerization alone can switch migration direction only at high strength.
show that actin polymerization alone can affect a switch in direction only at high strength
Actin polymerization alone can switch migration direction only at high strength.
show that actin polymerization alone can affect a switch in direction only at high strength
Actin polymerization alone can switch migration direction only at high strength.
show that actin polymerization alone can affect a switch in direction only at high strength
Actin polymerization alone can switch migration direction only at high strength.
show that actin polymerization alone can affect a switch in direction only at high strength
Actin polymerization alone can switch migration direction only at high strength.
show that actin polymerization alone can affect a switch in direction only at high strength
Actin polymerization alone can switch migration direction only at high strength.
show that actin polymerization alone can affect a switch in direction only at high strength
A one-dimensional active gel model predicts bistability between sessile and motile cell states when adhesion and contractility are sufficiently large and balanced.
Our model predicts bistability between sessile and motile solutions when cell adhesion and contractility are sufficiently large and in balance.
A one-dimensional active gel model predicts bistability between sessile and motile cell states when adhesion and contractility are sufficiently large and balanced.
Our model predicts bistability between sessile and motile solutions when cell adhesion and contractility are sufficiently large and in balance.
A one-dimensional active gel model predicts bistability between sessile and motile cell states when adhesion and contractility are sufficiently large and balanced.
Our model predicts bistability between sessile and motile solutions when cell adhesion and contractility are sufficiently large and in balance.
A one-dimensional active gel model predicts bistability between sessile and motile cell states when adhesion and contractility are sufficiently large and balanced.
Our model predicts bistability between sessile and motile solutions when cell adhesion and contractility are sufficiently large and in balance.
A one-dimensional active gel model predicts bistability between sessile and motile cell states when adhesion and contractility are sufficiently large and balanced.
Our model predicts bistability between sessile and motile solutions when cell adhesion and contractility are sufficiently large and in balance.
A one-dimensional active gel model predicts bistability between sessile and motile cell states when adhesion and contractility are sufficiently large and balanced.
Our model predicts bistability between sessile and motile solutions when cell adhesion and contractility are sufficiently large and in balance.
A one-dimensional active gel model predicts bistability between sessile and motile cell states when adhesion and contractility are sufficiently large and balanced.
Our model predicts bistability between sessile and motile solutions when cell adhesion and contractility are sufficiently large and in balance.
A one-dimensional active gel model predicts bistability between sessile and motile cell states when adhesion and contractility are sufficiently large and balanced.
Our model predicts bistability between sessile and motile solutions when cell adhesion and contractility are sufficiently large and in balance.
A one-dimensional active gel model predicts bistability between sessile and motile cell states when adhesion and contractility are sufficiently large and balanced.
Our model predicts bistability between sessile and motile solutions when cell adhesion and contractility are sufficiently large and in balance.
A one-dimensional active gel model predicts bistability between sessile and motile cell states when adhesion and contractility are sufficiently large and balanced.
Our model predicts bistability between sessile and motile solutions when cell adhesion and contractility are sufficiently large and in balance.
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Approval Evidence
1 source1 linked approval claimfirst-pass slug optogenetic-control-of-contractility
Optogenetics makes it experimentally possible to spatially control contraction and possibly cell migration too. We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility.
Source:
model predictionsupports
Optogenetic activation or inhibition of contractility can switch cells between sessile and motile states at realistic parameter values.
We show that one can switch between the different states at realistic parameter values via optogenetic activation or inhibition of contractility
Source:
Comparisons
Source-backed strengths
The main strength is that the model predicts state switching at realistic parameter values through optogenetic activation or inhibition of contractility. It also provides a mechanistic framework in which sufficiently large and balanced adhesion and contractility generate bistability between sessile and motile states.
optogenetic control of contractility and Method for efficient synthesis of phycocyanobilin in cultured mammalian cells address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Strengths here: may avoid an exogenous cofactor requirement.
Compared with optogenetic inhibition of Delta
optogenetic control of contractility and optogenetic inhibition of Delta address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: optogenetic inhibition; same primary input modality: light
Compared with optogenetic tool regulating endogenous formin mDia
optogenetic control of contractility and optogenetic tool regulating endogenous formin mDia address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: optogenetic activation; same primary input modality: light
Ranked Citations
- 1.