Source 1primary paper2013Science
Toolkit/optogenetic tool regulating endogenous formin mDia
optogenetic tool regulating endogenous formin mDia
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
This optogenetic method regulates endogenous nuclear formin mDia by photoreactive release of formin autoinhibition. In the cited Science study, mDia activation induced rapid and reversible nuclear actin network assembly, followed by MAL nuclear accumulation and SRF activity.
Usefulness & Problems
Why this is useful
The tool provides light-based control over endogenous nuclear mDia activity, enabling temporal perturbation of nuclear actin assembly without relying solely on constitutively active formin constructs. It is useful for dissecting how nuclear actin dynamics couple to MAL localization and SRF-dependent transcriptional responses.
Source:
The endogenous formin mDia was regulated with an optogenetic tool, which allowed for photoreactive release of nuclear formin autoinhibition.
Problem solved
It addresses the problem of how to acutely and reversibly activate endogenous nuclear formin mDia to test causal links between formin activity, nuclear actin network assembly, and downstream MAL-SRF signaling. The supplied evidence specifically supports control of nuclear formin autoinhibition by light.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The available evidence indicates that the method acts on endogenous nuclear mDia through photoreactive release of formin autoinhibition. Practical details such as required light wavelength, chromophore dependence, expression strategy, and construct design are not described in the supplied evidence.
The supplied evidence is limited to a single cited study and does not specify the photoreceptor module, illumination wavelength, construct architecture, or quantitative performance metrics. Evidence for use outside the reported nuclear mDia context, or for independent replication, is not provided here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Activated mDia promotes rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity.
Activated mDia promoted rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity.
Activated mDia promotes rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity.
Activated mDia promoted rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity.
Activated mDia promotes rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity.
Activated mDia promoted rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity.
Activated mDia promotes rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity.
Activated mDia promoted rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity.
Activated mDia promotes rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity.
Activated mDia promoted rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity.
Activated mDia promotes rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity.
Activated mDia promoted rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity.
Activated mDia promotes rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity.
Activated mDia promoted rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity.
An optogenetic tool enabled photoreactive release of nuclear formin autoinhibition for endogenous mDia.
The endogenous formin mDia was regulated with an optogenetic tool, which allowed for photoreactive release of nuclear formin autoinhibition.
An optogenetic tool enabled photoreactive release of nuclear formin autoinhibition for endogenous mDia.
The endogenous formin mDia was regulated with an optogenetic tool, which allowed for photoreactive release of nuclear formin autoinhibition.
An optogenetic tool enabled photoreactive release of nuclear formin autoinhibition for endogenous mDia.
The endogenous formin mDia was regulated with an optogenetic tool, which allowed for photoreactive release of nuclear formin autoinhibition.
An optogenetic tool enabled photoreactive release of nuclear formin autoinhibition for endogenous mDia.
The endogenous formin mDia was regulated with an optogenetic tool, which allowed for photoreactive release of nuclear formin autoinhibition.
An optogenetic tool enabled photoreactive release of nuclear formin autoinhibition for endogenous mDia.
The endogenous formin mDia was regulated with an optogenetic tool, which allowed for photoreactive release of nuclear formin autoinhibition.
An optogenetic tool enabled photoreactive release of nuclear formin autoinhibition for endogenous mDia.
The endogenous formin mDia was regulated with an optogenetic tool, which allowed for photoreactive release of nuclear formin autoinhibition.
An optogenetic tool enabled photoreactive release of nuclear formin autoinhibition for endogenous mDia.
The endogenous formin mDia was regulated with an optogenetic tool, which allowed for photoreactive release of nuclear formin autoinhibition.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug optogenetic-tool-regulating-endogenous-formin-mdia
The endogenous formin mDia was regulated with an optogenetic tool, which allowed for photoreactive release of nuclear formin autoinhibition.
Source:
downstream effectsupports
Activated mDia promotes rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity.
Activated mDia promoted rapid and reversible nuclear actin network assembly, subsequent MAL nuclear accumulation, and SRF activity.
Source:
tool enabled controlsupports
An optogenetic tool enabled photoreactive release of nuclear formin autoinhibition for endogenous mDia.
The endogenous formin mDia was regulated with an optogenetic tool, which allowed for photoreactive release of nuclear formin autoinhibition.
Source:
Comparisons
Source-backed strengths
The reported activation was rapid and reversible, and it was applied to endogenous mDia rather than only exogenous engineered formin variants. The study linked this perturbation to clear downstream readouts: nuclear actin network assembly, MAL nuclear accumulation, and SRF activity.
Ranked Citations
- 1.