Source 1primary paper2022Journal of Biological Chemistry
Toolkit/Optogenetic PLDs
Optogenetic PLDs
Also known as: optoPLD
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Optogenetic PLDs use light-mediated heterodimerization to recruit a bacterial PLD to desired organelle membranes
Usefulness & Problems
No literature-backed usefulness or problem-fit explainer has been materialized for this record yet.
Published Workflows
Click chemistry and optogenetic approaches to visualize and manipulate phosphatidic acid signaling
2022Objective: Create and apply a suite of chemical tools to image and perturb phosphatidic acid signaling with high spatiotemporal precision.
Why it works: The workflow combines chemically selective labeling of PLD-derived reporter lipids with light-controlled perturbation strategies, which the abstract presents as suitable for the rapid metabolism and trafficking of phosphatidic acid.
PLD-mediated phosphatidic acid productionlight-mediated recruitment of bacterial PLD to organelle membraneslight-controlled modulation of phosphatidic acid analog shape and bioactivityclick chemistrybioorthogonal taggingoptogeneticsphotoswitchable lipid analog design
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
HeterodimerizationTechniques
No technique tags yet.
Target processes
signalingInput: Light
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The described chemical tools are presented as useful for studying GPCR-Gq signaling, discovering regulators of PLD signaling, tracking intracellular lipid transport pathways, and elucidating oncogenic signaling roles for phosphatidic acid.
We highlight select applications of these tools for studying GPCR-Gq signaling, discovering regulators of PLD signaling, tracking intracellular lipid transport pathways, and elucidating new oncogenic signaling roles for PA.
Optogenetic PLDs manipulate phosphatidic acid signaling by light-mediated heterodimerization that recruits a bacterial PLD to desired organelle membranes.
Optogenetic PLDs use light-mediated heterodimerization to recruit a bacterial PLD to desired organelle membranes
Approval Evidence
1 source2 linked approval claimsfirst-pass slug optogenetic-plds
Optogenetic PLDs use light-mediated heterodimerization to recruit a bacterial PLD to desired organelle membranes
Source:
application scopesupports
The described chemical tools are presented as useful for studying GPCR-Gq signaling, discovering regulators of PLD signaling, tracking intracellular lipid transport pathways, and elucidating oncogenic signaling roles for phosphatidic acid.
We highlight select applications of these tools for studying GPCR-Gq signaling, discovering regulators of PLD signaling, tracking intracellular lipid transport pathways, and elucidating new oncogenic signaling roles for PA.
Source:
tool functionsupports
Optogenetic PLDs manipulate phosphatidic acid signaling by light-mediated heterodimerization that recruits a bacterial PLD to desired organelle membranes.
Optogenetic PLDs use light-mediated heterodimerization to recruit a bacterial PLD to desired organelle membranes
Source:
Comparisons
No literature-backed comparison notes have been materialized for this record yet.
Ranked Citations
- 1.