Source 1primary paper2023
Toolkit/optogenetic split transcription factors
optogenetic split transcription factors
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Optogenetic split transcription factors are multi-component light-responsive transcriptional regulators developed in Saccharomyces cerevisiae. In the cited yeast toolkit, split transcription factor designs incorporated cryptochrome and Enhanced Magnet light-sensitive dimerizers, and an optimized Enhanced Magnet variant improved light-sensitive gene expression.
Usefulness & Problems
Why this is useful
These tools enable light-controlled regulation of transcription in yeast through split transcription factor architectures. The associated toolkit is useful because laboratory automation and modular cloning support high-throughput construction and characterization of many designs.
Source:
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Problem solved
This tool helps address the challenge of building and testing light-responsive transcription regulators in Saccharomyces cerevisiae at scale. It specifically supports systematic assembly and evaluation of split transcription factor variants using optogenetic dimerizers.
Problem links
Need precise spatiotemporal control with light input
DerivedOptogenetic split transcription factors are multi-component light-responsive regulators for transcription in Saccharomyces cerevisiae. In the cited yeast toolkit, cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factor designs, and laboratory automation with modular cloning enabled their high-throughput construction and characterization.
Need tighter control over gene expression timing or amplitude
DerivedOptogenetic split transcription factors are multi-component light-responsive regulators for transcription in Saccharomyces cerevisiae. In the cited yeast toolkit, cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factor designs, and laboratory automation with modular cloning enabled their high-throughput construction and characterization.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
transcriptionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: multi componentswitch architecture: recruitmentswitch architecture: split
The reported implementations are split transcription factor constructs incorporating cryptochrome or Enhanced Magnet light-sensitive dimerizers. Construction and characterization were enabled by a modular cloning scheme and laboratory automation in Saccharomyces cerevisiae, but the supplied evidence does not specify construct architecture, promoters, cofactors, or delivery details.
The supplied evidence is limited to a yeast toolkit context and does not describe performance outside Saccharomyces cerevisiae. Quantitative metrics, illumination parameters, dynamic range, background activity, reversibility, and independent replication are not provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
An optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
The yeast optogenetic toolkit was expanded to include variants of cryptochromes and Enhanced Magnets.
We expand the yeast optogenetic toolkit to include variants of the cryptochromes and Enhanced Magnets
Approval Evidence
1 source2 linked approval claimsfirst-pass slug optogenetic-split-transcription-factors
enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae
Source:
capabilitysupports
Laboratory automation combined with a modular cloning scheme enables high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae.
We combine laboratory automation and a modular cloning scheme to enable high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae .
Source:
design integrationsupports
Cryptochrome and Enhanced Magnet light-sensitive dimerizers were incorporated into split transcription factors.
incorporate these light-sensitive dimerizers into split transcription factors
Source:
Comparisons
Source-backed strengths
The reported platform supports high-throughput construction and characterization of optogenetic split transcription factors in Saccharomyces cerevisiae. It integrates at least two light-sensitive dimerizer systems, cryptochrome and Enhanced Magnet, and an optimized Enhanced Magnet transcription factor showed improved light-sensitive gene expression.
Source:
We use this approach to rationally design and test an optimized Enhanced Magnet transcription factor with improved light-sensitive gene expression.
Compared with iLID/SspB
optogenetic split transcription factors and iLID/SspB address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; shared mechanisms: heterodimerization; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Compared with LITEs (Light-inducible transcriptional effectors)
optogenetic split transcription factors and LITEs (Light-inducible transcriptional effectors) address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; shared mechanisms: heterodimerization; same primary input modality: light
Compared with LOVpep/ePDZb
optogenetic split transcription factors and LOVpep/ePDZb address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; shared mechanisms: heterodimerization; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.