Source 1review2018Frontiers in Genetics
Toolkit/optogenetic systems adapted to regulate gene expression
optogenetic systems adapted to regulate gene expression
Also known as: optogenetics repertoire, optogenetic systems
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Optogenetic systems adapted to regulate gene expression are genetically engineered photosensing protein systems that respond to specific wavelengths of light to control molecular activities. The reviewed repertoire includes systems used to regulate gene expression in both unicellular and multicellular organisms, enabling high spatial and temporal precision.
Usefulness & Problems
Why this is useful
These systems are useful because light provides acute control over molecular activities with fine spatial and temporal resolution. They address limitations of classical inducible systems, which lack the accuracy needed to study events that depend on timing, activation duration, or cellular localization.
Source:
By exploiting genetically engineered photo sensing proteins that respond to specific wavelengths, we can now provide acute control of numerous molecular activities with unprecedented precision.
Problem solved
This tool class helps solve the problem of controlling gene expression with precise timing and subcellular or tissue-level localization. It is positioned as an alternative to classical inducible systems when molecular events require fine spatial and temporal accuracy.
Problem links
Need inducible protein relocalization or recruitment
DerivedOptogenetic systems adapted to regulate gene expression are genetically engineered photosensing protein systems that respond to specific wavelengths of light to control molecular activities. The reviewed repertoire includes systems used to regulate gene expression in both unicellular and multicellular organisms, enabling high spatial and temporal precision.
Need precise spatiotemporal control with light input
DerivedOptogenetic systems adapted to regulate gene expression are genetically engineered photosensing protein systems that respond to specific wavelengths of light to control molecular activities. The reviewed repertoire includes systems used to regulate gene expression in both unicellular and multicellular organisms, enabling high spatial and temporal precision.
Need tighter control over protein production
DerivedOptogenetic systems adapted to regulate gene expression are genetically engineered photosensing protein systems that respond to specific wavelengths of light to control molecular activities. The reviewed repertoire includes systems used to regulate gene expression in both unicellular and multicellular organisms, enabling high spatial and temporal precision.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
localizationtranslationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: multi component
The available evidence supports that these are genetically engineered, multi-component photosensing protein systems activated by specific wavelengths of light. The supplied material does not specify particular proteins, chromophores, construct architectures, delivery methods, or host-specific implementation requirements.
The source notes current and future challenges in translating these optogenetic tools to more complex organisms. It does not provide specific performance metrics, individual system comparisons, or detailed constraints for particular photosensors in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Genetically engineered photosensing proteins that respond to specific wavelengths can provide acute control of molecular activities with high spatial and temporal precision.
By exploiting genetically engineered photo sensing proteins that respond to specific wavelengths, we can now provide acute control of numerous molecular activities with unprecedented precision.
Classical inducible systems lack fine spatial and temporal accuracy, limiting study of molecular events that depend on timing, activation duration, or cellular localization.
Unfortunately, most of the classical inducible systems lack fine spatial and temporal accuracy, thereby limiting the study of molecular events that strongly depend on time, duration of activation, or cellular localization.
Current optogenetic systems have been adapted to regulate gene expression in both unicellular and multicellular organisms.
In this review, we present a comprehensive breakdown of all of the current optogenetic systems adapted to regulate gene expression in both unicellular and multicellular organisms.
The review discusses current and future challenges in successful translation of these optogenetic tools to more complex organisms.
We focus on the advantages and disadvantages of these different tools and discuss current and future challenges in the successful translation to more complex organisms.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug optogenetic-systems-adapted-to-regulate-gene-expression
In this review, we present a comprehensive breakdown of all of the current optogenetic systems adapted to regulate gene expression in both unicellular and multicellular organisms.
Source:
capability summarysupports
Genetically engineered photosensing proteins that respond to specific wavelengths can provide acute control of molecular activities with high spatial and temporal precision.
By exploiting genetically engineered photo sensing proteins that respond to specific wavelengths, we can now provide acute control of numerous molecular activities with unprecedented precision.
Source:
limitation summarysupports
Classical inducible systems lack fine spatial and temporal accuracy, limiting study of molecular events that depend on timing, activation duration, or cellular localization.
Unfortunately, most of the classical inducible systems lack fine spatial and temporal accuracy, thereby limiting the study of molecular events that strongly depend on time, duration of activation, or cellular localization.
Source:
scope summarysupports
Current optogenetic systems have been adapted to regulate gene expression in both unicellular and multicellular organisms.
In this review, we present a comprehensive breakdown of all of the current optogenetic systems adapted to regulate gene expression in both unicellular and multicellular organisms.
Source:
translation challenge summarysupports
The review discusses current and future challenges in successful translation of these optogenetic tools to more complex organisms.
We focus on the advantages and disadvantages of these different tools and discuss current and future challenges in the successful translation to more complex organisms.
Source:
Comparisons
Source-backed strengths
The principal strength supported by the source is high spatial and temporal precision in regulating molecular activities through wavelength-responsive photosensing proteins. The repertoire has been adapted for gene-expression control across both unicellular and multicellular organisms, indicating applicability across biological contexts.
Compared with cLIPS1
optogenetic systems adapted to regulate gene expression and cLIPS1 address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation control, translation_control; same primary input modality: light
Compared with LiGluR-MAG0(460)
optogenetic systems adapted to regulate gene expression and LiGluR-MAG0(460) address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control; same primary input modality: light
Strengths here: looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Compared with prime-editing
optogenetic systems adapted to regulate gene expression and prime-editing address a similar problem space because they share localization, translation.
Shared frame: same top-level item type; shared target processes: localization, translation; shared mechanisms: translation_control; same primary input modality: light
Strengths here: may avoid an exogenous cofactor requirement.
Relative tradeoffs: appears more independently replicated.
Ranked Citations
- 1.