Source 1review2014Biological Chemistry
Toolkit/optogenetic tool
optogenetic tool
Also known as: currently available optogenetic tools, optogenetic, genetically encoded, and light-responsive tools
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Optogenetic tools are genetically encoded, light-responsive systems that enable control of biological processes with the spatiotemporal precision of light. In the cited context, they are described specifically as light-controlled gene expression technologies in mammalian cells.
Usefulness & Problems
Why this is useful
These tools are useful because they provide light-based control with high spatiotemporal precision. The cited review presents them as an approach that overcomes limitations of chemical-controlled switches for regulating gene expression in mammalian cells.
Problem solved
They help solve the limited spatiotemporal resolution of chemical-controlled molecular switches. According to the source, this limitation arises because chemical inducers diffuse freely, whereas optogenetic systems can be controlled with light.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
light-dependent switchingTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The evidence supports that these systems are genetically encoded and used for light-controlled gene expression in mammalian cells. No specific wavelengths, photoreceptors, cofactors, construct architectures, or delivery methods are provided in the supplied evidence.
The provided evidence does not specify performance limitations of the optogenetic tools themselves beyond the general review context. It only explicitly contrasts them with chemical-controlled switches, whose inducers diffuse freely and therefore limit spatiotemporal resolution.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Optogenetic genetically encoded light-responsive tools address the spatiotemporal-resolution limitations of chemical-controlled switches by enabling control with light.
These limitations have recently been addressed by the development of optogenetic, genetically encoded, and light-responsive tools that can be controlled with the unprecedented spatiotemporal precision of light.
Chemical-controlled molecular switches are limited in spatiotemporal resolution because their inducers diffuse freely.
However, these tools are limited in terms of their spatiotemporal resolution due to freely diffusing inducers.
Chemical-controlled molecular switches are limited in spatiotemporal resolution because their inducers diffuse freely.
However, these tools are limited in terms of their spatiotemporal resolution due to freely diffusing inducers.
Chemical-controlled molecular switches are limited in spatiotemporal resolution because their inducers diffuse freely.
However, these tools are limited in terms of their spatiotemporal resolution due to freely diffusing inducers.
Chemical-controlled molecular switches are limited in spatiotemporal resolution because their inducers diffuse freely.
However, these tools are limited in terms of their spatiotemporal resolution due to freely diffusing inducers.
Chemical-controlled molecular switches are limited in spatiotemporal resolution because their inducers diffuse freely.
However, these tools are limited in terms of their spatiotemporal resolution due to freely diffusing inducers.
Chemical-controlled molecular switches are limited in spatiotemporal resolution because their inducers diffuse freely.
However, these tools are limited in terms of their spatiotemporal resolution due to freely diffusing inducers.
Chemical-controlled molecular switches are limited in spatiotemporal resolution because their inducers diffuse freely.
However, these tools are limited in terms of their spatiotemporal resolution due to freely diffusing inducers.
The review focuses on recent developments in light-controlled gene expression technologies and offers guidance for selecting a suitable gene expression system.
Then, we focus on recent developments in light-controlled gene expression technologies and provide the reader with a guideline for choosing the most suitable gene expression system.
The review focuses on recent developments in light-controlled gene expression technologies and offers guidance for selecting a suitable gene expression system.
Then, we focus on recent developments in light-controlled gene expression technologies and provide the reader with a guideline for choosing the most suitable gene expression system.
The review focuses on recent developments in light-controlled gene expression technologies and offers guidance for selecting a suitable gene expression system.
Then, we focus on recent developments in light-controlled gene expression technologies and provide the reader with a guideline for choosing the most suitable gene expression system.
The review focuses on recent developments in light-controlled gene expression technologies and offers guidance for selecting a suitable gene expression system.
Then, we focus on recent developments in light-controlled gene expression technologies and provide the reader with a guideline for choosing the most suitable gene expression system.
The review focuses on recent developments in light-controlled gene expression technologies and offers guidance for selecting a suitable gene expression system.
Then, we focus on recent developments in light-controlled gene expression technologies and provide the reader with a guideline for choosing the most suitable gene expression system.
The review focuses on recent developments in light-controlled gene expression technologies and offers guidance for selecting a suitable gene expression system.
Then, we focus on recent developments in light-controlled gene expression technologies and provide the reader with a guideline for choosing the most suitable gene expression system.
The review focuses on recent developments in light-controlled gene expression technologies and offers guidance for selecting a suitable gene expression system.
Then, we focus on recent developments in light-controlled gene expression technologies and provide the reader with a guideline for choosing the most suitable gene expression system.
Approval Evidence
1 source1 linked approval claimfirst-pass slug optogenetic-tool
These limitations have recently been addressed by the development of optogenetic, genetically encoded, and light-responsive tools that can be controlled with the unprecedented spatiotemporal precision of light.
Source:
advantagesupports
Optogenetic genetically encoded light-responsive tools address the spatiotemporal-resolution limitations of chemical-controlled switches by enabling control with light.
These limitations have recently been addressed by the development of optogenetic, genetically encoded, and light-responsive tools that can be controlled with the unprecedented spatiotemporal precision of light.
Source:
Comparisons
Source-backed strengths
The main reported strength is unprecedented spatiotemporal precision afforded by light control. The source also indicates their relevance for light-controlled gene expression in mammalian cells and discusses recent developments in selecting suitable gene expression systems.
Source:
These limitations have recently been addressed by the development of optogenetic, genetically encoded, and light-responsive tools that can be controlled with the unprecedented spatiotemporal precision of light.
Ranked Citations
- 1.