Source 1primary paper2019
Toolkit/optogenetic transcriptional control
optogenetic transcriptional control
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Optogenetic transcriptional control is a light-responsive gene regulation approach used in an integrative cell-ablation strategy in zebrafish embryos. The available evidence indicates that it enabled targeted cell elimination with spatial and temporal precision.
Usefulness & Problems
Why this is useful
This approach is useful for controlling transcription with light in order to drive cell ablation at defined times and locations in a developing vertebrate system. The cited application demonstrates utility for precise perturbation of selected cells in zebrafish embryos.
Source:
Using this integrative approach, we have ablated cells in zebrafish embryos with spatial and temporal precision.
Problem solved
It addresses the problem of ablating cells with limited spatial and temporal precision in vivo. In the reported zebrafish embryo application, optogenetic transcriptional control was used to achieve targeted cell elimination with both spatial and temporal control.
Source:
Using this integrative approach, we have ablated cells in zebrafish embryos with spatial and temporal precision.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Mechanisms
optogenetic transcriptional controlTechniques
No technique tags yet.
Target processes
transcriptionInput: Light
Implementation Constraints
The available evidence specifies use in zebrafish embryos and indicates integration into an optogenetic ablative strategy. No construct design, promoter system, illumination parameters, cofactors, or delivery method are described in the supplied evidence.
The supplied evidence is limited to a single application-level statement and a title, without details on the photoreceptor system, transcriptional architecture, light wavelength, kinetics, dynamic range, or off-target effects. Validation is only evidenced in zebrafish embryos, and independent replication is not provided in the supplied material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Using the integrative optogenetic ablative approach, the authors ablated cells in zebrafish embryos with spatial and temporal precision.
Using this integrative approach, we have ablated cells in zebrafish embryos with spatial and temporal precision.
Using the integrative optogenetic ablative approach, the authors ablated cells in zebrafish embryos with spatial and temporal precision.
Using this integrative approach, we have ablated cells in zebrafish embryos with spatial and temporal precision.
Using the integrative optogenetic ablative approach, the authors ablated cells in zebrafish embryos with spatial and temporal precision.
Using this integrative approach, we have ablated cells in zebrafish embryos with spatial and temporal precision.
Using the integrative optogenetic ablative approach, the authors ablated cells in zebrafish embryos with spatial and temporal precision.
Using this integrative approach, we have ablated cells in zebrafish embryos with spatial and temporal precision.
Using the integrative optogenetic ablative approach, the authors ablated cells in zebrafish embryos with spatial and temporal precision.
Using this integrative approach, we have ablated cells in zebrafish embryos with spatial and temporal precision.
Using the integrative optogenetic ablative approach, the authors ablated cells in zebrafish embryos with spatial and temporal precision.
Using this integrative approach, we have ablated cells in zebrafish embryos with spatial and temporal precision.
Using the integrative optogenetic ablative approach, the authors ablated cells in zebrafish embryos with spatial and temporal precision.
Using this integrative approach, we have ablated cells in zebrafish embryos with spatial and temporal precision.
Approval Evidence
1 source1 linked approval claimfirst-pass slug optogenetic-transcriptional-control
Targeted cell ablation in zebrafish using optogenetic transcriptional control
Source:
application resultsupports
Using the integrative optogenetic ablative approach, the authors ablated cells in zebrafish embryos with spatial and temporal precision.
Using this integrative approach, we have ablated cells in zebrafish embryos with spatial and temporal precision.
Source:
Comparisons
Source-backed strengths
The reported strength is spatially and temporally precise cell ablation in zebrafish embryos. The evidence supports successful use in an integrative ablative approach, but does not provide quantitative performance metrics in the supplied material.
Ranked Citations
- 1.