Source 1primary paper2014Nature Methods
Toolkit/Optopatch
Optopatch
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The web research summary states that the anchor paper explicitly names Optopatch as the coexpression vector/platform enabling cross-talk-free genetically targeted all-optical electrophysiology.
Usefulness & Problems
No literature-backed usefulness or problem-fit explainer has been materialized for this record yet.
Published Workflows
Objective: Enable all-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins for optical stimulation and optical voltage recording in the same genetically targeted system.
Why it works: The source scaffold indicates that the platform pairs QuasAr voltage indicators with the CheRiff actuator and emphasizes spectral orthogonality and cross-talk-free operation.
microbial rhodopsin-based voltage-sensitive fluorescencechannelrhodopsin-based optical actuationall-optical electrophysiologycoexpression platform design
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
optical membrane depolarization via channelrhodopsin actuationoptical voltage sensing via archaerhodopsin-based voltage indicatorsspectral orthogonality for cross-talk reductionTarget processes
recombinationselectionInput: Light
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2review2017Trends in biotechnology
Source 3review2016Biophysical Journal
Ranked Claims
The review discusses bPAC as an optogenetic tool for cAMP control.
The review discusses iGluSnFR as a neurotransmitter reporter relevant to synaptic function assays.
The review discusses optoXRs as optogenetic tools for GPCR signaling in discovery-oriented applications.
The review describes Optopatch as pairing CheRiff with QuasAr voltage indicators.
This review centers optogenetic and all-optical electrophysiology approaches for phenotypic screening in drug discovery.
GCaMP6s is used in the review context as an activity reporter example for zebrafish brain imaging.
The anchor figure caption explicitly cites GCaMP6s for zebrafish brain activity imaging.
LOVpep and ePDZ are presented in the review context as a light-induced intracellular trafficking control system.
The anchor figure caption names LOVpep and ePDZ in a light-induced trafficking example.
Optopatch is presented in the review context as an all-optical electrophysiology system with CheRiff and QuasAr2 as named components.
PubMed figure captions ... explicitly mention tool/component names used in the review, especially Optopatch/CheRiff/QuasAr2.
pMag and nMag are presented in the review context as a light-induced dimerization pair used to reconstitute split Cas9 in photoactivatable genome editing.
The anchor figure caption names pMag as one half of the light-induced dimerization pair used to reconstitute split Cas9 ... nMag as the partner to pMag.
CheRiff is an engineered channelrhodopsin actuator that is spectrally orthogonal to QuasAr indicators.
QuasAr1 and QuasAr2 are evolved archaerhodopsin-based voltage indicators named as core components of the anchor paper.
Optopatch is a coexpression platform enabling cross-talk-free genetically targeted all-optical electrophysiology.
This paper presents all-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins.
Approval Evidence
3 sources5 linked approval claimsfirst-pass slug optopatch
The review explicitly centers optogenetic/all-optical electrophysiology for phenotypic screening, especially the Optopatch platform pairing CheRiff with QuasAr voltage indicators.
Source:
The supplied web research summary states that PubMed figure captions for the anchor review explicitly mention Optopatch and that the review spans all-optical electrophysiology.
Source:
The web research summary states that the anchor paper explicitly names Optopatch as the coexpression vector/platform enabling cross-talk-free genetically targeted all-optical electrophysiology.
Source:
component compositionsupports
The review describes Optopatch as pairing CheRiff with QuasAr voltage indicators.
Source:
review scopesupports
This review centers optogenetic and all-optical electrophysiology approaches for phenotypic screening in drug discovery.
Source:
tool composition or rolesupports
Optopatch is presented in the review context as an all-optical electrophysiology system with CheRiff and QuasAr2 as named components.
PubMed figure captions ... explicitly mention tool/component names used in the review, especially Optopatch/CheRiff/QuasAr2.
Source:
system capabilitysupports
Optopatch is a coexpression platform enabling cross-talk-free genetically targeted all-optical electrophysiology.
Source:
tool introductionsupports
This paper presents all-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins.
Source:
Comparisons
No literature-backed comparison notes have been materialized for this record yet.
Ranked Citations
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