Source 1primary paper2017Chemical Science
Toolkit/OptoPB
OptoPB
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
OptoPB is an optogenetic multi-component switch generated by installing photosensitivity into engineered minimal phosphoinositide-binding domains. It enables rapid and reversible light-controlled protein translocation, plasma membrane targeting, and inter-membrane tethering at membrane contact sites in living cells.
Usefulness & Problems
Why this is useful
OptoPB is useful for optical control of membrane-associated localization and interorganellar communication with reversibility in living cells. It also serves as a scaffold for grafting lipid-binding domains to dissect determinants of protein-lipid interactions in cells.
Source:
to enable rapid and reversible control of protein translocation and inter-membrane tethering at MCSs
Source:
By installing photosensitivity into a series of engineered PI-binding domains with minimal sizes, we have created an optogenetic toolkit (designated as 'OptoPB')
Source:
When tethered to the ER membrane with the insertion of flexible spacers, OptoPB can be applied to reversibly photo-tune the gap distances at nanometer scales between the two organellar membranes at MCSs, and to gauge the distance requirement for the free diffusion of protein complexes into MCSs.
Source:
These genetically-encoded, single-component tools can be used as scaffolds for grafting lipid-binding domains to dissect molecular determinants that govern protein-lipid interactions in living cells.
Problem solved
OptoPB addresses the problem of controlling phosphoinositide-dependent membrane recruitment and membrane tethering with high temporal precision using light. The cited work specifically positions it as a way to manipulate protein translocation and membrane contact site tethering at nanoscales.
Source:
When tethered to the ER membrane with the insertion of flexible spacers, OptoPB can be applied to reversibly photo-tune the gap distances at nanometer scales between the two organellar membranes at MCSs, and to gauge the distance requirement for the free diffusion of protein complexes into MCSs.
Source:
These genetically-encoded, single-component tools can be used as scaffolds for grafting lipid-binding domains to dissect molecular determinants that govern protein-lipid interactions in living cells.
Source:
we have demonstrated the use of OptoPB as a versatile fusion tag to photomanipulate protein translocation toward PM for reprogramming of PI metabolism
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
light-controlled protein translocationphosphoinositide-dependent membrane localizationreversible membrane tetheringTechniques
Computational DesignTarget processes
localizationrecombinationInput: Light
Implementation Constraints
OptoPB was created by installing photosensitivity into engineered minimal phosphoinositide-binding domains, indicating a domain-fusion-based construct architecture. The available evidence supports use in living cells, but does not specify cofactors, expression systems, or detailed construct design parameters.
The provided evidence does not report quantitative performance metrics, specific illumination wavelengths, or comparative benchmarking against other optogenetic systems. Independent replication and validation outside the source study are not documented in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
OptoPB enables rapid and reversible control of protein translocation and inter-membrane tethering at membrane contact sites.
to enable rapid and reversible control of protein translocation and inter-membrane tethering at MCSs
OptoPB enables rapid and reversible control of protein translocation and inter-membrane tethering at membrane contact sites.
to enable rapid and reversible control of protein translocation and inter-membrane tethering at MCSs
OptoPB enables rapid and reversible control of protein translocation and inter-membrane tethering at membrane contact sites.
to enable rapid and reversible control of protein translocation and inter-membrane tethering at MCSs
OptoPB enables rapid and reversible control of protein translocation and inter-membrane tethering at membrane contact sites.
to enable rapid and reversible control of protein translocation and inter-membrane tethering at MCSs
OptoPB enables rapid and reversible control of protein translocation and inter-membrane tethering at membrane contact sites.
to enable rapid and reversible control of protein translocation and inter-membrane tethering at MCSs
OptoPB enables rapid and reversible control of protein translocation and inter-membrane tethering at membrane contact sites.
to enable rapid and reversible control of protein translocation and inter-membrane tethering at MCSs
OptoPB enables rapid and reversible control of protein translocation and inter-membrane tethering at membrane contact sites.
to enable rapid and reversible control of protein translocation and inter-membrane tethering at MCSs
The authors created an optogenetic toolkit called OptoPB by installing photosensitivity into engineered minimal PI-binding domains.
By installing photosensitivity into a series of engineered PI-binding domains with minimal sizes, we have created an optogenetic toolkit (designated as 'OptoPB')
The authors created an optogenetic toolkit called OptoPB by installing photosensitivity into engineered minimal PI-binding domains.
By installing photosensitivity into a series of engineered PI-binding domains with minimal sizes, we have created an optogenetic toolkit (designated as 'OptoPB')
The authors created an optogenetic toolkit called OptoPB by installing photosensitivity into engineered minimal PI-binding domains.
By installing photosensitivity into a series of engineered PI-binding domains with minimal sizes, we have created an optogenetic toolkit (designated as 'OptoPB')
The authors created an optogenetic toolkit called OptoPB by installing photosensitivity into engineered minimal PI-binding domains.
By installing photosensitivity into a series of engineered PI-binding domains with minimal sizes, we have created an optogenetic toolkit (designated as 'OptoPB')
The authors created an optogenetic toolkit called OptoPB by installing photosensitivity into engineered minimal PI-binding domains.
By installing photosensitivity into a series of engineered PI-binding domains with minimal sizes, we have created an optogenetic toolkit (designated as 'OptoPB')
The authors created an optogenetic toolkit called OptoPB by installing photosensitivity into engineered minimal PI-binding domains.
By installing photosensitivity into a series of engineered PI-binding domains with minimal sizes, we have created an optogenetic toolkit (designated as 'OptoPB')
The authors created an optogenetic toolkit called OptoPB by installing photosensitivity into engineered minimal PI-binding domains.
By installing photosensitivity into a series of engineered PI-binding domains with minimal sizes, we have created an optogenetic toolkit (designated as 'OptoPB')
ER-tethered OptoPB with flexible spacers can reversibly photo-tune nanometer-scale gap distances between organellar membranes at membrane contact sites and gauge distance requirements for free diffusion of protein complexes into these sites.
When tethered to the ER membrane with the insertion of flexible spacers, OptoPB can be applied to reversibly photo-tune the gap distances at nanometer scales between the two organellar membranes at MCSs, and to gauge the distance requirement for the free diffusion of protein complexes into MCSs.
inter membrane gap distance nanometer scales
ER-tethered OptoPB with flexible spacers can reversibly photo-tune nanometer-scale gap distances between organellar membranes at membrane contact sites and gauge distance requirements for free diffusion of protein complexes into these sites.
When tethered to the ER membrane with the insertion of flexible spacers, OptoPB can be applied to reversibly photo-tune the gap distances at nanometer scales between the two organellar membranes at MCSs, and to gauge the distance requirement for the free diffusion of protein complexes into MCSs.
inter membrane gap distance nanometer scales
ER-tethered OptoPB with flexible spacers can reversibly photo-tune nanometer-scale gap distances between organellar membranes at membrane contact sites and gauge distance requirements for free diffusion of protein complexes into these sites.
When tethered to the ER membrane with the insertion of flexible spacers, OptoPB can be applied to reversibly photo-tune the gap distances at nanometer scales between the two organellar membranes at MCSs, and to gauge the distance requirement for the free diffusion of protein complexes into MCSs.
inter membrane gap distance nanometer scales
ER-tethered OptoPB with flexible spacers can reversibly photo-tune nanometer-scale gap distances between organellar membranes at membrane contact sites and gauge distance requirements for free diffusion of protein complexes into these sites.
When tethered to the ER membrane with the insertion of flexible spacers, OptoPB can be applied to reversibly photo-tune the gap distances at nanometer scales between the two organellar membranes at MCSs, and to gauge the distance requirement for the free diffusion of protein complexes into MCSs.
inter membrane gap distance nanometer scales
ER-tethered OptoPB with flexible spacers can reversibly photo-tune nanometer-scale gap distances between organellar membranes at membrane contact sites and gauge distance requirements for free diffusion of protein complexes into these sites.
When tethered to the ER membrane with the insertion of flexible spacers, OptoPB can be applied to reversibly photo-tune the gap distances at nanometer scales between the two organellar membranes at MCSs, and to gauge the distance requirement for the free diffusion of protein complexes into MCSs.
inter membrane gap distance nanometer scales
ER-tethered OptoPB with flexible spacers can reversibly photo-tune nanometer-scale gap distances between organellar membranes at membrane contact sites and gauge distance requirements for free diffusion of protein complexes into these sites.
When tethered to the ER membrane with the insertion of flexible spacers, OptoPB can be applied to reversibly photo-tune the gap distances at nanometer scales between the two organellar membranes at MCSs, and to gauge the distance requirement for the free diffusion of protein complexes into MCSs.
inter membrane gap distance nanometer scales
ER-tethered OptoPB with flexible spacers can reversibly photo-tune nanometer-scale gap distances between organellar membranes at membrane contact sites and gauge distance requirements for free diffusion of protein complexes into these sites.
When tethered to the ER membrane with the insertion of flexible spacers, OptoPB can be applied to reversibly photo-tune the gap distances at nanometer scales between the two organellar membranes at MCSs, and to gauge the distance requirement for the free diffusion of protein complexes into MCSs.
inter membrane gap distance nanometer scales
OptoPB can be used as a scaffold for grafting lipid-binding domains to dissect determinants of protein-lipid interactions in living cells.
These genetically-encoded, single-component tools can be used as scaffolds for grafting lipid-binding domains to dissect molecular determinants that govern protein-lipid interactions in living cells.
OptoPB can be used as a scaffold for grafting lipid-binding domains to dissect determinants of protein-lipid interactions in living cells.
These genetically-encoded, single-component tools can be used as scaffolds for grafting lipid-binding domains to dissect molecular determinants that govern protein-lipid interactions in living cells.
OptoPB can be used as a scaffold for grafting lipid-binding domains to dissect determinants of protein-lipid interactions in living cells.
These genetically-encoded, single-component tools can be used as scaffolds for grafting lipid-binding domains to dissect molecular determinants that govern protein-lipid interactions in living cells.
OptoPB can be used as a scaffold for grafting lipid-binding domains to dissect determinants of protein-lipid interactions in living cells.
These genetically-encoded, single-component tools can be used as scaffolds for grafting lipid-binding domains to dissect molecular determinants that govern protein-lipid interactions in living cells.
OptoPB can be used as a scaffold for grafting lipid-binding domains to dissect determinants of protein-lipid interactions in living cells.
These genetically-encoded, single-component tools can be used as scaffolds for grafting lipid-binding domains to dissect molecular determinants that govern protein-lipid interactions in living cells.
OptoPB can be used as a scaffold for grafting lipid-binding domains to dissect determinants of protein-lipid interactions in living cells.
These genetically-encoded, single-component tools can be used as scaffolds for grafting lipid-binding domains to dissect molecular determinants that govern protein-lipid interactions in living cells.
OptoPB can be used as a scaffold for grafting lipid-binding domains to dissect determinants of protein-lipid interactions in living cells.
These genetically-encoded, single-component tools can be used as scaffolds for grafting lipid-binding domains to dissect molecular determinants that govern protein-lipid interactions in living cells.
OptoPB can function as a versatile fusion tag to photomanipulate protein translocation toward the plasma membrane for reprogramming phosphoinositide metabolism.
we have demonstrated the use of OptoPB as a versatile fusion tag to photomanipulate protein translocation toward PM for reprogramming of PI metabolism
OptoPB can function as a versatile fusion tag to photomanipulate protein translocation toward the plasma membrane for reprogramming phosphoinositide metabolism.
we have demonstrated the use of OptoPB as a versatile fusion tag to photomanipulate protein translocation toward PM for reprogramming of PI metabolism
OptoPB can function as a versatile fusion tag to photomanipulate protein translocation toward the plasma membrane for reprogramming phosphoinositide metabolism.
we have demonstrated the use of OptoPB as a versatile fusion tag to photomanipulate protein translocation toward PM for reprogramming of PI metabolism
OptoPB can function as a versatile fusion tag to photomanipulate protein translocation toward the plasma membrane for reprogramming phosphoinositide metabolism.
we have demonstrated the use of OptoPB as a versatile fusion tag to photomanipulate protein translocation toward PM for reprogramming of PI metabolism
OptoPB can function as a versatile fusion tag to photomanipulate protein translocation toward the plasma membrane for reprogramming phosphoinositide metabolism.
we have demonstrated the use of OptoPB as a versatile fusion tag to photomanipulate protein translocation toward PM for reprogramming of PI metabolism
OptoPB can function as a versatile fusion tag to photomanipulate protein translocation toward the plasma membrane for reprogramming phosphoinositide metabolism.
we have demonstrated the use of OptoPB as a versatile fusion tag to photomanipulate protein translocation toward PM for reprogramming of PI metabolism
OptoPB can function as a versatile fusion tag to photomanipulate protein translocation toward the plasma membrane for reprogramming phosphoinositide metabolism.
we have demonstrated the use of OptoPB as a versatile fusion tag to photomanipulate protein translocation toward PM for reprogramming of PI metabolism
Approval Evidence
1 source5 linked approval claimsfirst-pass slug optopb
we have created an optogenetic toolkit (designated as 'OptoPB')
Source:
functional capabilitysupports
OptoPB enables rapid and reversible control of protein translocation and inter-membrane tethering at membrane contact sites.
to enable rapid and reversible control of protein translocation and inter-membrane tethering at MCSs
Source:
tool creationsupports
The authors created an optogenetic toolkit called OptoPB by installing photosensitivity into engineered minimal PI-binding domains.
By installing photosensitivity into a series of engineered PI-binding domains with minimal sizes, we have created an optogenetic toolkit (designated as 'OptoPB')
Source:
use casesupports
ER-tethered OptoPB with flexible spacers can reversibly photo-tune nanometer-scale gap distances between organellar membranes at membrane contact sites and gauge distance requirements for free diffusion of protein complexes into these sites.
When tethered to the ER membrane with the insertion of flexible spacers, OptoPB can be applied to reversibly photo-tune the gap distances at nanometer scales between the two organellar membranes at MCSs, and to gauge the distance requirement for the free diffusion of protein complexes into MCSs.
Source:
use casesupports
OptoPB can be used as a scaffold for grafting lipid-binding domains to dissect determinants of protein-lipid interactions in living cells.
These genetically-encoded, single-component tools can be used as scaffolds for grafting lipid-binding domains to dissect molecular determinants that govern protein-lipid interactions in living cells.
Source:
use casesupports
OptoPB can function as a versatile fusion tag to photomanipulate protein translocation toward the plasma membrane for reprogramming phosphoinositide metabolism.
we have demonstrated the use of OptoPB as a versatile fusion tag to photomanipulate protein translocation toward PM for reprogramming of PI metabolism
Source:
Comparisons
Source-backed strengths
The reported strengths are rapid and reversible control of protein translocation and inter-membrane tethering. The tool was also presented as enabling plasma membrane targeting and as a modular scaffold for studying protein-lipid interaction determinants in living cells.
Ranked Citations
- 1.