Source 1primary paper2023
Toolkit/OptoProfilin
OptoProfilin
Also known as: optically activated profilin
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
OptoProfilin is an optically activated profilin-based, single-component biosensor reported in 2023. It was described as an optically triggered sensor of applied cellular stress in select immortalized cell lines and presented as the first profilin-based optogenetic biosensor for stress-induced cytoskeletal changes.
Usefulness & Problems
Why this is useful
OptoProfilin is useful as a genetically encoded light-responsive biosensor for probing applied cellular stress and associated cytoskeletal responses in living cells. Its single-component architecture may simplify deployment relative to multi-part systems, although the supplied evidence does not quantify this advantage.
Source:
we describe the design and development of an optogenetic iteration of profilin
Problem solved
This tool addresses the need for an optically controlled biosensor that reports applied cellular stress in cells. The cited work specifically positions it as a way to sense stress-induced changes in the cytoskeleton using profilin.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
Computational DesignTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The supplied evidence indicates that OptoProfilin is a single-component construct and was used in select immortalized cell lines. No details are provided here on chromophore requirements, excitation wavelength, expression strategy, delivery method, or construct architecture beyond its profilin basis and optical activation.
The available evidence is narrow and does not specify the optical parameters, molecular design, dynamic range, response kinetics, or quantitative performance. Validation is only stated for select immortalized cell lines, with no independent replication or broader organismal testing provided in the supplied material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
OptoProfilin can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines.
OptoProfilin') can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines
OptoProfilin can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines.
OptoProfilin') can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines
OptoProfilin can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines.
OptoProfilin') can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines
OptoProfilin can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines.
OptoProfilin') can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines
OptoProfilin can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines.
OptoProfilin') can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines
OptoProfilin can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines.
OptoProfilin') can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines
OptoProfilin can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines.
OptoProfilin') can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines
OptoProfilin is a single component biosensor.
Notably, OptoProfilin is a single component biosensor
OptoProfilin is a single component biosensor.
Notably, OptoProfilin is a single component biosensor
OptoProfilin is a single component biosensor.
Notably, OptoProfilin is a single component biosensor
OptoProfilin is a single component biosensor.
Notably, OptoProfilin is a single component biosensor
OptoProfilin is a single component biosensor.
Notably, OptoProfilin is a single component biosensor
OptoProfilin is a single component biosensor.
Notably, OptoProfilin is a single component biosensor
OptoProfilin is a single component biosensor.
Notably, OptoProfilin is a single component biosensor
This work is presented as the first example of profilin as an optogenetic biosensor of stress-induced changes in the cytoskeleton.
this, to our knowledge, is the first example of profilin as an optogenetic biosensor of stress-induced changes in the cytoskeleton
This work is presented as the first example of profilin as an optogenetic biosensor of stress-induced changes in the cytoskeleton.
this, to our knowledge, is the first example of profilin as an optogenetic biosensor of stress-induced changes in the cytoskeleton
This work is presented as the first example of profilin as an optogenetic biosensor of stress-induced changes in the cytoskeleton.
this, to our knowledge, is the first example of profilin as an optogenetic biosensor of stress-induced changes in the cytoskeleton
This work is presented as the first example of profilin as an optogenetic biosensor of stress-induced changes in the cytoskeleton.
this, to our knowledge, is the first example of profilin as an optogenetic biosensor of stress-induced changes in the cytoskeleton
This work is presented as the first example of profilin as an optogenetic biosensor of stress-induced changes in the cytoskeleton.
this, to our knowledge, is the first example of profilin as an optogenetic biosensor of stress-induced changes in the cytoskeleton
This work is presented as the first example of profilin as an optogenetic biosensor of stress-induced changes in the cytoskeleton.
this, to our knowledge, is the first example of profilin as an optogenetic biosensor of stress-induced changes in the cytoskeleton
This work is presented as the first example of profilin as an optogenetic biosensor of stress-induced changes in the cytoskeleton.
this, to our knowledge, is the first example of profilin as an optogenetic biosensor of stress-induced changes in the cytoskeleton
OptoProfilin is an optogenetic iteration of profilin.
we describe the design and development of an optogenetic iteration of profilin
OptoProfilin is an optogenetic iteration of profilin.
we describe the design and development of an optogenetic iteration of profilin
OptoProfilin is an optogenetic iteration of profilin.
we describe the design and development of an optogenetic iteration of profilin
OptoProfilin is an optogenetic iteration of profilin.
we describe the design and development of an optogenetic iteration of profilin
OptoProfilin is an optogenetic iteration of profilin.
we describe the design and development of an optogenetic iteration of profilin
OptoProfilin is an optogenetic iteration of profilin.
we describe the design and development of an optogenetic iteration of profilin
OptoProfilin is an optogenetic iteration of profilin.
we describe the design and development of an optogenetic iteration of profilin
Approval Evidence
1 source4 linked approval claimsfirst-pass slug optoprofilin
We demonstrate that this optically activated profilin ('OptoProfilin') can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines. Notably, OptoProfilin is a single component biosensor
Source:
biosensor functionsupports
OptoProfilin can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines.
OptoProfilin') can act as an optically triggered biosensor of applied cellular stress in select immortalized cell lines
Source:
component architecturesupports
OptoProfilin is a single component biosensor.
Notably, OptoProfilin is a single component biosensor
Source:
noveltysupports
This work is presented as the first example of profilin as an optogenetic biosensor of stress-induced changes in the cytoskeleton.
this, to our knowledge, is the first example of profilin as an optogenetic biosensor of stress-induced changes in the cytoskeleton
Source:
tool descriptionsupports
OptoProfilin is an optogenetic iteration of profilin.
we describe the design and development of an optogenetic iteration of profilin
Source:
Comparisons
Source-backed strengths
The main reported strengths are that OptoProfilin is optically activated, functions as a single-component biosensor, and was demonstrated in select immortalized cell lines. The work also claims novelty as the first example of profilin used as an optogenetic biosensor of stress-induced cytoskeletal change.
Source:
this, to our knowledge, is the first example of profilin as an optogenetic biosensor of stress-induced changes in the cytoskeleton
Ranked Citations
- 1.