Source 1primary paper2024ACS Synthetic Biology
Toolkit/OptoREACT
OptoREACT
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
OptoREACT is a light-dependent extracellular receptor activation system for nonengineered cells. It combines phytochrome B with a PIF6-coupled antibody fragment to bind cell-surface receptors and, upon illumination, drive receptor oligomerization and activation, including the T cell receptor on Jurkat and primary human T cells.
Usefulness & Problems
Why this is useful
OptoREACT enables optogenetic control of cell-surface receptor signaling without genetic engineering of the target cells. This is useful for studying and manipulating receptor activation in nonengineered human cells through an extracellular, light-gated assembly strategy.
Source:
we developed a system for the light-dependent extracellular activation of cell surface receptors of nonengineered cells termed OptoREACT
Problem solved
OptoREACT addresses the problem of how to activate receptors on nonengineered cells with light. The reported implementation solves this by using an antibody fragment for receptor targeting and a light-dependent phytochrome B–PIF6 interaction to induce receptor oligomerization extracellularly.
Source:
we developed a system for the light-dependent extracellular activation of cell surface receptors of nonengineered cells termed OptoREACT
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
light-dependent phytochrome b-pif6 bindinglight-dependent phytochrome b–pif6 bindingOligomerizationreceptor oligomerizationTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
OptoREACT uses the light-dependent interaction between phytochrome B and PIF6 as its core switch. A PIF6-coupled antibody fragment binds the target receptor, and phytochrome B clustering was implemented either through streptavidin-mediated tetramerization or by immobilization on cell surfaces.
The supplied evidence is limited to a single 2024 study and focuses on T cell receptor activation in Jurkat and primary human T cells. No quantitative performance metrics, spectral details, reversibility data, or validation across multiple receptor classes are provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Phytochrome B clustering in OptoREACT was implemented either by streptavidin-mediated tetramerization or by immobilization on cell surfaces.
For clustering of PhyB, we either used tetramerization by streptavidin or immobilized PhyB on the surface of cells
Phytochrome B clustering in OptoREACT was implemented either by streptavidin-mediated tetramerization or by immobilization on cell surfaces.
For clustering of PhyB, we either used tetramerization by streptavidin or immobilized PhyB on the surface of cells
Phytochrome B clustering in OptoREACT was implemented either by streptavidin-mediated tetramerization or by immobilization on cell surfaces.
For clustering of PhyB, we either used tetramerization by streptavidin or immobilized PhyB on the surface of cells
Phytochrome B clustering in OptoREACT was implemented either by streptavidin-mediated tetramerization or by immobilization on cell surfaces.
For clustering of PhyB, we either used tetramerization by streptavidin or immobilized PhyB on the surface of cells
Phytochrome B clustering in OptoREACT was implemented either by streptavidin-mediated tetramerization or by immobilization on cell surfaces.
For clustering of PhyB, we either used tetramerization by streptavidin or immobilized PhyB on the surface of cells
Phytochrome B clustering in OptoREACT was implemented either by streptavidin-mediated tetramerization or by immobilization on cell surfaces.
For clustering of PhyB, we either used tetramerization by streptavidin or immobilized PhyB on the surface of cells
Phytochrome B clustering in OptoREACT was implemented either by streptavidin-mediated tetramerization or by immobilization on cell surfaces.
For clustering of PhyB, we either used tetramerization by streptavidin or immobilized PhyB on the surface of cells
In OptoREACT, a PIF6-coupled antibody fragment binds the T cell receptor of Jurkat or primary human T cells and, upon illumination, clustered phytochrome B induces receptor oligomerization and activation.
a PIF6-coupled antibody fragment binds the T cell receptor (TCR) of Jurkat or primary human T cells, which upon illumination is bound by clustered phytochrome B to induce receptor oligomerization and activation
In OptoREACT, a PIF6-coupled antibody fragment binds the T cell receptor of Jurkat or primary human T cells and, upon illumination, clustered phytochrome B induces receptor oligomerization and activation.
a PIF6-coupled antibody fragment binds the T cell receptor (TCR) of Jurkat or primary human T cells, which upon illumination is bound by clustered phytochrome B to induce receptor oligomerization and activation
In OptoREACT, a PIF6-coupled antibody fragment binds the T cell receptor of Jurkat or primary human T cells and, upon illumination, clustered phytochrome B induces receptor oligomerization and activation.
a PIF6-coupled antibody fragment binds the T cell receptor (TCR) of Jurkat or primary human T cells, which upon illumination is bound by clustered phytochrome B to induce receptor oligomerization and activation
In OptoREACT, a PIF6-coupled antibody fragment binds the T cell receptor of Jurkat or primary human T cells and, upon illumination, clustered phytochrome B induces receptor oligomerization and activation.
a PIF6-coupled antibody fragment binds the T cell receptor (TCR) of Jurkat or primary human T cells, which upon illumination is bound by clustered phytochrome B to induce receptor oligomerization and activation
In OptoREACT, a PIF6-coupled antibody fragment binds the T cell receptor of Jurkat or primary human T cells and, upon illumination, clustered phytochrome B induces receptor oligomerization and activation.
a PIF6-coupled antibody fragment binds the T cell receptor (TCR) of Jurkat or primary human T cells, which upon illumination is bound by clustered phytochrome B to induce receptor oligomerization and activation
In OptoREACT, a PIF6-coupled antibody fragment binds the T cell receptor of Jurkat or primary human T cells and, upon illumination, clustered phytochrome B induces receptor oligomerization and activation.
a PIF6-coupled antibody fragment binds the T cell receptor (TCR) of Jurkat or primary human T cells, which upon illumination is bound by clustered phytochrome B to induce receptor oligomerization and activation
In OptoREACT, a PIF6-coupled antibody fragment binds the T cell receptor of Jurkat or primary human T cells and, upon illumination, clustered phytochrome B induces receptor oligomerization and activation.
a PIF6-coupled antibody fragment binds the T cell receptor (TCR) of Jurkat or primary human T cells, which upon illumination is bound by clustered phytochrome B to induce receptor oligomerization and activation
OptoREACT is based on the light-dependent interaction between phytochrome B and PIF6.
based on the light-dependent protein interaction of A. thaliana phytochrome B (PhyB) with PIF6
OptoREACT is based on the light-dependent interaction between phytochrome B and PIF6.
based on the light-dependent protein interaction of A. thaliana phytochrome B (PhyB) with PIF6
OptoREACT is based on the light-dependent interaction between phytochrome B and PIF6.
based on the light-dependent protein interaction of A. thaliana phytochrome B (PhyB) with PIF6
OptoREACT is based on the light-dependent interaction between phytochrome B and PIF6.
based on the light-dependent protein interaction of A. thaliana phytochrome B (PhyB) with PIF6
OptoREACT is based on the light-dependent interaction between phytochrome B and PIF6.
based on the light-dependent protein interaction of A. thaliana phytochrome B (PhyB) with PIF6
OptoREACT is based on the light-dependent interaction between phytochrome B and PIF6.
based on the light-dependent protein interaction of A. thaliana phytochrome B (PhyB) with PIF6
OptoREACT is based on the light-dependent interaction between phytochrome B and PIF6.
based on the light-dependent protein interaction of A. thaliana phytochrome B (PhyB) with PIF6
The authors anticipate that this extracellular optogenetic approach will be applicable to light-controlled activation of additional cell surface receptors in primary, nonengineered cells.
We anticipate that this extracellular optogenetic approach will be applicable for the light-controlled activation of further cell surface receptors in primary, nonengineered cells
The authors anticipate that this extracellular optogenetic approach will be applicable to light-controlled activation of additional cell surface receptors in primary, nonengineered cells.
We anticipate that this extracellular optogenetic approach will be applicable for the light-controlled activation of further cell surface receptors in primary, nonengineered cells
The authors anticipate that this extracellular optogenetic approach will be applicable to light-controlled activation of additional cell surface receptors in primary, nonengineered cells.
We anticipate that this extracellular optogenetic approach will be applicable for the light-controlled activation of further cell surface receptors in primary, nonengineered cells
The authors anticipate that this extracellular optogenetic approach will be applicable to light-controlled activation of additional cell surface receptors in primary, nonengineered cells.
We anticipate that this extracellular optogenetic approach will be applicable for the light-controlled activation of further cell surface receptors in primary, nonengineered cells
The authors anticipate that this extracellular optogenetic approach will be applicable to light-controlled activation of additional cell surface receptors in primary, nonengineered cells.
We anticipate that this extracellular optogenetic approach will be applicable for the light-controlled activation of further cell surface receptors in primary, nonengineered cells
The authors anticipate that this extracellular optogenetic approach will be applicable to light-controlled activation of additional cell surface receptors in primary, nonengineered cells.
We anticipate that this extracellular optogenetic approach will be applicable for the light-controlled activation of further cell surface receptors in primary, nonengineered cells
The authors anticipate that this extracellular optogenetic approach will be applicable to light-controlled activation of additional cell surface receptors in primary, nonengineered cells.
We anticipate that this extracellular optogenetic approach will be applicable for the light-controlled activation of further cell surface receptors in primary, nonengineered cells
OptoREACT is a system for light-dependent extracellular activation of cell surface receptors on nonengineered cells.
we developed a system for the light-dependent extracellular activation of cell surface receptors of nonengineered cells termed OptoREACT
OptoREACT is a system for light-dependent extracellular activation of cell surface receptors on nonengineered cells.
we developed a system for the light-dependent extracellular activation of cell surface receptors of nonengineered cells termed OptoREACT
OptoREACT is a system for light-dependent extracellular activation of cell surface receptors on nonengineered cells.
we developed a system for the light-dependent extracellular activation of cell surface receptors of nonengineered cells termed OptoREACT
OptoREACT is a system for light-dependent extracellular activation of cell surface receptors on nonengineered cells.
we developed a system for the light-dependent extracellular activation of cell surface receptors of nonengineered cells termed OptoREACT
OptoREACT is a system for light-dependent extracellular activation of cell surface receptors on nonengineered cells.
we developed a system for the light-dependent extracellular activation of cell surface receptors of nonengineered cells termed OptoREACT
OptoREACT is a system for light-dependent extracellular activation of cell surface receptors on nonengineered cells.
we developed a system for the light-dependent extracellular activation of cell surface receptors of nonengineered cells termed OptoREACT
OptoREACT is a system for light-dependent extracellular activation of cell surface receptors on nonengineered cells.
we developed a system for the light-dependent extracellular activation of cell surface receptors of nonengineered cells termed OptoREACT
Approval Evidence
1 source5 linked approval claimsfirst-pass slug optoreact
we developed a system for the light-dependent extracellular activation of cell surface receptors of nonengineered cells termed OptoREACT
Source:
implementationsupports
Phytochrome B clustering in OptoREACT was implemented either by streptavidin-mediated tetramerization or by immobilization on cell surfaces.
For clustering of PhyB, we either used tetramerization by streptavidin or immobilized PhyB on the surface of cells
Source:
mechanismsupports
In OptoREACT, a PIF6-coupled antibody fragment binds the T cell receptor of Jurkat or primary human T cells and, upon illumination, clustered phytochrome B induces receptor oligomerization and activation.
a PIF6-coupled antibody fragment binds the T cell receptor (TCR) of Jurkat or primary human T cells, which upon illumination is bound by clustered phytochrome B to induce receptor oligomerization and activation
Source:
mechanismsupports
OptoREACT is based on the light-dependent interaction between phytochrome B and PIF6.
based on the light-dependent protein interaction of A. thaliana phytochrome B (PhyB) with PIF6
Source:
prospective applicabilitysupports
The authors anticipate that this extracellular optogenetic approach will be applicable to light-controlled activation of additional cell surface receptors in primary, nonengineered cells.
We anticipate that this extracellular optogenetic approach will be applicable for the light-controlled activation of further cell surface receptors in primary, nonengineered cells
Source:
tool developmentsupports
OptoREACT is a system for light-dependent extracellular activation of cell surface receptors on nonengineered cells.
we developed a system for the light-dependent extracellular activation of cell surface receptors of nonengineered cells termed OptoREACT
Source:
Comparisons
Source-backed strengths
The system was reported to function on nonengineered cells and was specifically demonstrated on the T cell receptor in Jurkat and primary human T cells. Its modular architecture couples receptor-specific antibody binding to light-triggered assembly, and phytochrome B clustering was implemented by either streptavidin-mediated tetramerization or immobilization on cell surfaces.
Ranked Citations
- 1.