Toolkit/optoSynC

optoSynC

Multi-Component Switch·Research·Since 2022

Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

optoSynC is a non-ionic optogenetic silencer that uses light-evoked homo-oligomerization of cryptochrome CRY2 to cluster synaptic vesicles and silence synaptic transmission. It was benchmarked in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.

Usefulness & Problems

Why this is useful

optoSynC provides optical suppression of synaptic transmission without relying on ionic conductances, as it is described as a non-ionic optogenetic silencer. Its utility is supported by benchmarking across nematode, fish, and mammalian neuronal preparations.

Source:

optoSynC can inhibit exocytosis for several hours, at very low light intensities

Problem solved

optoSynC addresses the problem of silencing synaptic transmission with light by exploiting synaptic vesicle clustering rather than ion flux. The supplied evidence supports this specific use case but does not provide further detail on comparative performance or target contexts.

Taxonomy & Function

Primary hierarchy

Mechanism Branch

Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.

Techniques

No technique tags yet.

Target processes

No target processes tagged yet.

Input: Light

Implementation Constraints

Implementation requires a CRY2-based optogenetic construct because the silencing mechanism is explicitly attributed to cryptochrome CRY2 homo-oligomerization. The evidence indicates use in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons, but it does not report expression strategy, fusion partners, cofactors, or illumination parameters.

The provided evidence does not specify activation wavelength, kinetics, reversibility metrics, construct architecture, or quantitative silencing performance. Independent replication is not shown in the supplied material, and validation details beyond the named systems are absent.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Supporting Sources

Ranked Claims

Claim 1benchmark scopesupports2022Source 1needs review

optoSynC was benchmarked in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.

We benchmark this tool, optoSynC, in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.
Claim 2benchmark scopesupports2022Source 1needs review

optoSynC was benchmarked in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.

We benchmark this tool, optoSynC, in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.
Claim 3benchmark scopesupports2022Source 1needs review

optoSynC was benchmarked in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.

We benchmark this tool, optoSynC, in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.
Claim 4benchmark scopesupports2022Source 1needs review

optoSynC was benchmarked in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.

We benchmark this tool, optoSynC, in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.
Claim 5benchmark scopesupports2022Source 1needs review

optoSynC was benchmarked in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.

We benchmark this tool, optoSynC, in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.
Claim 6benchmark scopesupports2022Source 1needs review

optoSynC was benchmarked in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.

We benchmark this tool, optoSynC, in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.
Claim 7benchmark scopesupports2022Source 1needs review

optoSynC was benchmarked in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.

We benchmark this tool, optoSynC, in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.
Claim 8classificationsupports2022Source 1needs review

optoSynC is a highly efficient non-ionic optogenetic silencer.

optoSynC is a highly efficient, ‘non-ionic’ optogenetic silencer
Claim 9classificationsupports2022Source 1needs review

optoSynC is a highly efficient non-ionic optogenetic silencer.

optoSynC is a highly efficient, ‘non-ionic’ optogenetic silencer
Claim 10classificationsupports2022Source 1needs review

optoSynC is a highly efficient non-ionic optogenetic silencer.

optoSynC is a highly efficient, ‘non-ionic’ optogenetic silencer
Claim 11classificationsupports2022Source 1needs review

optoSynC is a highly efficient non-ionic optogenetic silencer.

optoSynC is a highly efficient, ‘non-ionic’ optogenetic silencer
Claim 12classificationsupports2022Source 1needs review

optoSynC is a highly efficient non-ionic optogenetic silencer.

optoSynC is a highly efficient, ‘non-ionic’ optogenetic silencer
Claim 13classificationsupports2022Source 1needs review

optoSynC is a highly efficient non-ionic optogenetic silencer.

optoSynC is a highly efficient, ‘non-ionic’ optogenetic silencer
Claim 14classificationsupports2022Source 1needs review

optoSynC is a highly efficient non-ionic optogenetic silencer.

optoSynC is a highly efficient, ‘non-ionic’ optogenetic silencer
Claim 15functional effectsupports2022Source 1needs review

optoSynC can inhibit exocytosis for several hours at very low light intensities.

optoSynC can inhibit exocytosis for several hours, at very low light intensities
duration of exocytosis inhibition several hourslight intensity requirement very low light intensities
Claim 16functional effectsupports2022Source 1needs review

optoSynC can inhibit exocytosis for several hours at very low light intensities.

optoSynC can inhibit exocytosis for several hours, at very low light intensities
duration of exocytosis inhibition several hourslight intensity requirement very low light intensities
Claim 17functional effectsupports2022Source 1needs review

optoSynC can inhibit exocytosis for several hours at very low light intensities.

optoSynC can inhibit exocytosis for several hours, at very low light intensities
duration of exocytosis inhibition several hourslight intensity requirement very low light intensities
Claim 18functional effectsupports2022Source 1needs review

optoSynC can inhibit exocytosis for several hours at very low light intensities.

optoSynC can inhibit exocytosis for several hours, at very low light intensities
duration of exocytosis inhibition several hourslight intensity requirement very low light intensities
Claim 19functional effectsupports2022Source 1needs review

optoSynC can inhibit exocytosis for several hours at very low light intensities.

optoSynC can inhibit exocytosis for several hours, at very low light intensities
duration of exocytosis inhibition several hourslight intensity requirement very low light intensities
Claim 20functional effectsupports2022Source 1needs review

optoSynC can inhibit exocytosis for several hours at very low light intensities.

optoSynC can inhibit exocytosis for several hours, at very low light intensities
duration of exocytosis inhibition several hourslight intensity requirement very low light intensities
Claim 21functional effectsupports2022Source 1needs review

optoSynC can inhibit exocytosis for several hours at very low light intensities.

optoSynC can inhibit exocytosis for several hours, at very low light intensities
duration of exocytosis inhibition several hourslight intensity requirement very low light intensities
Claim 22kineticssupports2022Source 1needs review

optoSynC-induced locomotion silencing occurs with tau_on of about 7.2 seconds and recovery after light-off with tau_off of about 6.5 minutes.

Locomotion silencing occurs with tauon ~7.2 s and recovers with tauoff ~6.5 min after light-off.
tau off 6.5 mintau on 7.2 s
Claim 23kineticssupports2022Source 1needs review

optoSynC-induced locomotion silencing occurs with tau_on of about 7.2 seconds and recovery after light-off with tau_off of about 6.5 minutes.

Locomotion silencing occurs with tauon ~7.2 s and recovers with tauoff ~6.5 min after light-off.
tau off 6.5 mintau on 7.2 s
Claim 24kineticssupports2022Source 1needs review

optoSynC-induced locomotion silencing occurs with tau_on of about 7.2 seconds and recovery after light-off with tau_off of about 6.5 minutes.

Locomotion silencing occurs with tauon ~7.2 s and recovers with tauoff ~6.5 min after light-off.
tau off 6.5 mintau on 7.2 s
Claim 25kineticssupports2022Source 1needs review

optoSynC-induced locomotion silencing occurs with tau_on of about 7.2 seconds and recovery after light-off with tau_off of about 6.5 minutes.

Locomotion silencing occurs with tauon ~7.2 s and recovers with tauoff ~6.5 min after light-off.
tau off 6.5 mintau on 7.2 s
Claim 26kineticssupports2022Source 1needs review

optoSynC-induced locomotion silencing occurs with tau_on of about 7.2 seconds and recovery after light-off with tau_off of about 6.5 minutes.

Locomotion silencing occurs with tauon ~7.2 s and recovers with tauoff ~6.5 min after light-off.
tau off 6.5 mintau on 7.2 s
Claim 27kineticssupports2022Source 1needs review

optoSynC-induced locomotion silencing occurs with tau_on of about 7.2 seconds and recovery after light-off with tau_off of about 6.5 minutes.

Locomotion silencing occurs with tauon ~7.2 s and recovers with tauoff ~6.5 min after light-off.
tau off 6.5 mintau on 7.2 s
Claim 28kineticssupports2022Source 1needs review

optoSynC-induced locomotion silencing occurs with tau_on of about 7.2 seconds and recovery after light-off with tau_off of about 6.5 minutes.

Locomotion silencing occurs with tauon ~7.2 s and recovers with tauoff ~6.5 min after light-off.
tau off 6.5 mintau on 7.2 s
Claim 29mechanismsupports2022Source 1needs review

optoSynC uses light-evoked homo-oligomerization of CRY2 to silence synaptic transmission by clustering synaptic vesicles.

Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs).
Claim 30mechanismsupports2022Source 1needs review

optoSynC uses light-evoked homo-oligomerization of CRY2 to silence synaptic transmission by clustering synaptic vesicles.

Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs).
Claim 31mechanismsupports2022Source 1needs review

optoSynC uses light-evoked homo-oligomerization of CRY2 to silence synaptic transmission by clustering synaptic vesicles.

Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs).
Claim 32mechanismsupports2022Source 1needs review

optoSynC uses light-evoked homo-oligomerization of CRY2 to silence synaptic transmission by clustering synaptic vesicles.

Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs).
Claim 33mechanismsupports2022Source 1needs review

optoSynC uses light-evoked homo-oligomerization of CRY2 to silence synaptic transmission by clustering synaptic vesicles.

Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs).
Claim 34mechanismsupports2022Source 1needs review

optoSynC uses light-evoked homo-oligomerization of CRY2 to silence synaptic transmission by clustering synaptic vesicles.

Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs).
Claim 35mechanismsupports2022Source 1needs review

optoSynC uses light-evoked homo-oligomerization of CRY2 to silence synaptic transmission by clustering synaptic vesicles.

Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs).
Claim 36mechanismsupports2022Source 1needs review

optoSynC uses light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission by clustering synaptic vesicles.

Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs).
Claim 37mechanismsupports2022Source 1needs review

optoSynC uses light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission by clustering synaptic vesicles.

Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs).
Claim 38mechanismsupports2022Source 1needs review

optoSynC uses light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission by clustering synaptic vesicles.

Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs).
Claim 39mechanismsupports2022Source 1needs review

optoSynC uses light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission by clustering synaptic vesicles.

Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs).
Claim 40mechanismsupports2022Source 1needs review

optoSynC uses light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission by clustering synaptic vesicles.

Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs).
Claim 41mechanismsupports2022Source 1needs review

optoSynC uses light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission by clustering synaptic vesicles.

Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs).
Claim 42mechanismsupports2022Source 1needs review

optoSynC uses light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission by clustering synaptic vesicles.

Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs).
Claim 43performancesupports2022Source 1needs review

optoSynC can inhibit exocytosis for several hours at very low light intensities.

Further, optoSynC can inhibit exocytosis for several hours, at very low light intensities.
exocytosis inhibition duration several hourslight intensity very low
Claim 44performancesupports2022Source 1needs review

optoSynC can inhibit exocytosis for several hours at very low light intensities.

Further, optoSynC can inhibit exocytosis for several hours, at very low light intensities.
exocytosis inhibition duration several hourslight intensity very low
Claim 45performancesupports2022Source 1needs review

optoSynC can inhibit exocytosis for several hours at very low light intensities.

Further, optoSynC can inhibit exocytosis for several hours, at very low light intensities.
exocytosis inhibition duration several hourslight intensity very low
Claim 46performancesupports2022Source 1needs review

optoSynC can inhibit exocytosis for several hours at very low light intensities.

Further, optoSynC can inhibit exocytosis for several hours, at very low light intensities.
exocytosis inhibition duration several hourslight intensity very low
Claim 47performancesupports2022Source 1needs review

optoSynC can inhibit exocytosis for several hours at very low light intensities.

Further, optoSynC can inhibit exocytosis for several hours, at very low light intensities.
exocytosis inhibition duration several hourslight intensity very low
Claim 48performancesupports2022Source 1needs review

optoSynC can inhibit exocytosis for several hours at very low light intensities.

Further, optoSynC can inhibit exocytosis for several hours, at very low light intensities.
exocytosis inhibition duration several hourslight intensity very low
Claim 49performancesupports2022Source 1needs review

optoSynC can inhibit exocytosis for several hours at very low light intensities.

Further, optoSynC can inhibit exocytosis for several hours, at very low light intensities.
exocytosis inhibition duration several hourslight intensity very low
Claim 50performancesupports2022Source 1needs review

optoSynC causes rapid locomotion silencing with an activation time constant of approximately 15 seconds and recovery after light-off with a time constant of approximately 10 minutes.

Locomotion silencing is rapid (tau on ∼15 s) and recovers quickly (tau off ∼10 min) after light-off.
locomotion silencing tau on 15 srecovery tau off after light-off 10 min
Claim 51performancesupports2022Source 1needs review

optoSynC causes rapid locomotion silencing with an activation time constant of approximately 15 seconds and recovery after light-off with a time constant of approximately 10 minutes.

Locomotion silencing is rapid (tau on ∼15 s) and recovers quickly (tau off ∼10 min) after light-off.
locomotion silencing tau on 15 srecovery tau off after light-off 10 min
Claim 52performancesupports2022Source 1needs review

optoSynC causes rapid locomotion silencing with an activation time constant of approximately 15 seconds and recovery after light-off with a time constant of approximately 10 minutes.

Locomotion silencing is rapid (tau on ∼15 s) and recovers quickly (tau off ∼10 min) after light-off.
locomotion silencing tau on 15 srecovery tau off after light-off 10 min
Claim 53performancesupports2022Source 1needs review

optoSynC causes rapid locomotion silencing with an activation time constant of approximately 15 seconds and recovery after light-off with a time constant of approximately 10 minutes.

Locomotion silencing is rapid (tau on ∼15 s) and recovers quickly (tau off ∼10 min) after light-off.
locomotion silencing tau on 15 srecovery tau off after light-off 10 min
Claim 54performancesupports2022Source 1needs review

optoSynC causes rapid locomotion silencing with an activation time constant of approximately 15 seconds and recovery after light-off with a time constant of approximately 10 minutes.

Locomotion silencing is rapid (tau on ∼15 s) and recovers quickly (tau off ∼10 min) after light-off.
locomotion silencing tau on 15 srecovery tau off after light-off 10 min
Claim 55performancesupports2022Source 1needs review

optoSynC causes rapid locomotion silencing with an activation time constant of approximately 15 seconds and recovery after light-off with a time constant of approximately 10 minutes.

Locomotion silencing is rapid (tau on ∼15 s) and recovers quickly (tau off ∼10 min) after light-off.
locomotion silencing tau on 15 srecovery tau off after light-off 10 min
Claim 56performancesupports2022Source 1needs review

optoSynC causes rapid locomotion silencing with an activation time constant of approximately 15 seconds and recovery after light-off with a time constant of approximately 10 minutes.

Locomotion silencing is rapid (tau on ∼15 s) and recovers quickly (tau off ∼10 min) after light-off.
locomotion silencing tau on 15 srecovery tau off after light-off 10 min
Claim 57performancesupports2022Source 1needs review

optoSynC clusters synaptic vesicles within 25 seconds.

optoSynC clusters SVs within 25 s
synaptic vesicle clustering time 25 s
Claim 58performancesupports2022Source 1needs review

optoSynC clusters synaptic vesicles within 25 seconds.

optoSynC clusters SVs within 25 s
synaptic vesicle clustering time 25 s
Claim 59performancesupports2022Source 1needs review

optoSynC clusters synaptic vesicles within 25 seconds.

optoSynC clusters SVs within 25 s
synaptic vesicle clustering time 25 s
Claim 60performancesupports2022Source 1needs review

optoSynC clusters synaptic vesicles within 25 seconds.

optoSynC clusters SVs within 25 s
synaptic vesicle clustering time 25 s
Claim 61performancesupports2022Source 1needs review

optoSynC clusters synaptic vesicles within 25 seconds.

optoSynC clusters SVs within 25 s
synaptic vesicle clustering time 25 s
Claim 62performancesupports2022Source 1needs review

optoSynC clusters synaptic vesicles within 25 seconds.

optoSynC clusters SVs within 25 s
synaptic vesicle clustering time 25 s
Claim 63performancesupports2022Source 1needs review

optoSynC clusters synaptic vesicles within 25 seconds.

optoSynC clusters SVs within 25 s
synaptic vesicle clustering time 25 s
Claim 64specificitysupports2022Source 1needs review

optoSynC does not affect ion currents, biochemistry, or synaptic proteins.

does not affect ion currents, biochemistry or synaptic proteins
Claim 65specificitysupports2022Source 1needs review

optoSynC does not affect ion currents, biochemistry, or synaptic proteins.

does not affect ion currents, biochemistry or synaptic proteins
Claim 66specificitysupports2022Source 1needs review

optoSynC does not affect ion currents, biochemistry, or synaptic proteins.

does not affect ion currents, biochemistry or synaptic proteins
Claim 67specificitysupports2022Source 1needs review

optoSynC does not affect ion currents, biochemistry, or synaptic proteins.

does not affect ion currents, biochemistry or synaptic proteins
Claim 68specificitysupports2022Source 1needs review

optoSynC does not affect ion currents, biochemistry, or synaptic proteins.

does not affect ion currents, biochemistry or synaptic proteins
Claim 69specificitysupports2022Source 1needs review

optoSynC does not affect ion currents, biochemistry, or synaptic proteins.

does not affect ion currents, biochemistry or synaptic proteins
Claim 70specificitysupports2022Source 1needs review

optoSynC does not affect ion currents, biochemistry, or synaptic proteins.

does not affect ion currents, biochemistry or synaptic proteins
Claim 71structural observationsupports2022Source 1needs review

optoSynC clusters synaptic vesicles, observable by electron microscopy.

optoSynC clusters SVs, observable by electron microscopy.
Claim 72structural observationsupports2022Source 1needs review

optoSynC clusters synaptic vesicles, observable by electron microscopy.

optoSynC clusters SVs, observable by electron microscopy.
Claim 73structural observationsupports2022Source 1needs review

optoSynC clusters synaptic vesicles, observable by electron microscopy.

optoSynC clusters SVs, observable by electron microscopy.
Claim 74structural observationsupports2022Source 1needs review

optoSynC clusters synaptic vesicles, observable by electron microscopy.

optoSynC clusters SVs, observable by electron microscopy.
Claim 75structural observationsupports2022Source 1needs review

optoSynC clusters synaptic vesicles, observable by electron microscopy.

optoSynC clusters SVs, observable by electron microscopy.
Claim 76structural observationsupports2022Source 1needs review

optoSynC clusters synaptic vesicles, observable by electron microscopy.

optoSynC clusters SVs, observable by electron microscopy.
Claim 77structural observationsupports2022Source 1needs review

optoSynC clusters synaptic vesicles, observable by electron microscopy.

optoSynC clusters SVs, observable by electron microscopy.

Approval Evidence

1 source11 linked approval claimsfirst-pass slug optosync
Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs). We benchmark this tool, optoSynC

Source:

Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs). We benchmark this tool, optoSynC, in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.

Source:

benchmark scopesupports

optoSynC was benchmarked in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.

We benchmark this tool, optoSynC, in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons.

Source:

classificationsupports

optoSynC is a highly efficient non-ionic optogenetic silencer.

optoSynC is a highly efficient, ‘non-ionic’ optogenetic silencer

Source:

functional effectsupports

optoSynC can inhibit exocytosis for several hours at very low light intensities.

optoSynC can inhibit exocytosis for several hours, at very low light intensities

Source:

kineticssupports

optoSynC-induced locomotion silencing occurs with tau_on of about 7.2 seconds and recovery after light-off with tau_off of about 6.5 minutes.

Locomotion silencing occurs with tauon ~7.2 s and recovers with tauoff ~6.5 min after light-off.

Source:

mechanismsupports

optoSynC uses light-evoked homo-oligomerization of CRY2 to silence synaptic transmission by clustering synaptic vesicles.

Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs).

Source:

mechanismsupports

optoSynC uses light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission by clustering synaptic vesicles.

Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering synaptic vesicles (SVs).

Source:

performancesupports

optoSynC can inhibit exocytosis for several hours at very low light intensities.

Further, optoSynC can inhibit exocytosis for several hours, at very low light intensities.

Source:

performancesupports

optoSynC causes rapid locomotion silencing with an activation time constant of approximately 15 seconds and recovery after light-off with a time constant of approximately 10 minutes.

Locomotion silencing is rapid (tau on ∼15 s) and recovers quickly (tau off ∼10 min) after light-off.

Source:

performancesupports

optoSynC clusters synaptic vesicles within 25 seconds.

optoSynC clusters SVs within 25 s

Source:

specificitysupports

optoSynC does not affect ion currents, biochemistry, or synaptic proteins.

does not affect ion currents, biochemistry or synaptic proteins

Source:

structural observationsupports

optoSynC clusters synaptic vesicles, observable by electron microscopy.

optoSynC clusters SVs, observable by electron microscopy.

Source:

Comparisons

Source-backed strengths

The source describes optoSynC as highly efficient and based on light-evoked CRY2 homo-oligomerization to silence synaptic transmission through synaptic vesicle clustering. It was benchmarked in Caenorhabditis elegans, zebrafish, and murine hippocampal neurons, indicating validation across multiple model systems.

Source:

Further, optoSynC can inhibit exocytosis for several hours, at very low light intensities.

Source:

Locomotion silencing is rapid (tau on ∼15 s) and recovers quickly (tau off ∼10 min) after light-off.

Source:

optoSynC clusters SVs within 25 s

Ranked Citations

  1. 1.
    StructuralSource 1Nature Communications2022Claim 1Claim 2Claim 3

    Seeded from load plan for claim claim_2. Extracted from this source document.