Source 1primary paper2017ACS Synthetic Biology
Toolkit/optoTGFBRs
optoTGFBRs
Also known as: optoTGFBRs system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
optoTGFBRs is an optogenetic multi-component switch developed to control TGF-β signaling with light. It enables precise spatiotemporal regulation of the pathway and has been used to drive selective and sequential activation in single cells.
Usefulness & Problems
Why this is useful
The system is useful for imposing defined light-input patterns on TGF-β signaling to study pathway dynamics with temporal and spatial precision. Reported use includes monitoring subcellular localization of TGF-β receptor and Smad2 proteins under different blue-light stimulation regimes.
Source:
Using the optoTGFBRs system, we show that TGF-β signaling can be selectively and sequentially activated in single cells through the modulation of the pattern of light stimulations.
Source:
Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-β signaling in time and space.
Source:
The spatial and temporal precision of light control will make the optoTGFBRs system as a powerful tool for quantitative analyses of TGF-β signaling at the single cell level.
Problem solved
optoTGFBRs addresses the problem of controlling TGF-β signaling in time and space with higher precision than conventional static perturbations. It also supports interrogation of how distinct light stimulation patterns shape single-cell signaling responses.
Source:
Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-β signaling in time and space.
Source:
The spatial and temporal precision of light control will make the optoTGFBRs system as a powerful tool for quantitative analyses of TGF-β signaling at the single cell level.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
localizationsignalingInput: Light
Implementation Constraints
The available evidence indicates that optoTGFBRs is a light-responsive, multi-component optogenetic system used with blue light stimulation. The provided material does not specify the photoreceptor modules, fusion architecture, cofactors, expression context, or delivery strategy.
The supplied evidence does not provide construct-level details, quantitative performance metrics, or comparisons against alternative TGF-β control methods. Independent replication, organismal scope, and long-term or in vivo validation are not documented in the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Using optoTGFBRs, TGF-β signaling can be selectively and sequentially activated in single cells by modulating the pattern of light stimulations.
Using the optoTGFBRs system, we show that TGF-β signaling can be selectively and sequentially activated in single cells through the modulation of the pattern of light stimulations.
Using optoTGFBRs, TGF-β signaling can be selectively and sequentially activated in single cells by modulating the pattern of light stimulations.
Using the optoTGFBRs system, we show that TGF-β signaling can be selectively and sequentially activated in single cells through the modulation of the pattern of light stimulations.
Using optoTGFBRs, TGF-β signaling can be selectively and sequentially activated in single cells by modulating the pattern of light stimulations.
Using the optoTGFBRs system, we show that TGF-β signaling can be selectively and sequentially activated in single cells through the modulation of the pattern of light stimulations.
Using optoTGFBRs, TGF-β signaling can be selectively and sequentially activated in single cells by modulating the pattern of light stimulations.
Using the optoTGFBRs system, we show that TGF-β signaling can be selectively and sequentially activated in single cells through the modulation of the pattern of light stimulations.
Using optoTGFBRs, TGF-β signaling can be selectively and sequentially activated in single cells by modulating the pattern of light stimulations.
Using the optoTGFBRs system, we show that TGF-β signaling can be selectively and sequentially activated in single cells through the modulation of the pattern of light stimulations.
Using optoTGFBRs, TGF-β signaling can be selectively and sequentially activated in single cells by modulating the pattern of light stimulations.
Using the optoTGFBRs system, we show that TGF-β signaling can be selectively and sequentially activated in single cells through the modulation of the pattern of light stimulations.
Using optoTGFBRs, TGF-β signaling can be selectively and sequentially activated in single cells by modulating the pattern of light stimulations.
Using the optoTGFBRs system, we show that TGF-β signaling can be selectively and sequentially activated in single cells through the modulation of the pattern of light stimulations.
The optoTGFBRs system was used to characterize TGF-β signaling dynamics in response to different patterns of blue light stimulation by monitoring subcellular localization of TGF-β receptor and Smad2 proteins.
By simultaneously monitoring the subcellular localization of TGF-β receptor and Smad2 proteins, we characterized the dynamics of TGF-β signaling in response to different patterns of blue light stimulations.
The optoTGFBRs system was used to characterize TGF-β signaling dynamics in response to different patterns of blue light stimulation by monitoring subcellular localization of TGF-β receptor and Smad2 proteins.
By simultaneously monitoring the subcellular localization of TGF-β receptor and Smad2 proteins, we characterized the dynamics of TGF-β signaling in response to different patterns of blue light stimulations.
The optoTGFBRs system was used to characterize TGF-β signaling dynamics in response to different patterns of blue light stimulation by monitoring subcellular localization of TGF-β receptor and Smad2 proteins.
By simultaneously monitoring the subcellular localization of TGF-β receptor and Smad2 proteins, we characterized the dynamics of TGF-β signaling in response to different patterns of blue light stimulations.
The optoTGFBRs system was used to characterize TGF-β signaling dynamics in response to different patterns of blue light stimulation by monitoring subcellular localization of TGF-β receptor and Smad2 proteins.
By simultaneously monitoring the subcellular localization of TGF-β receptor and Smad2 proteins, we characterized the dynamics of TGF-β signaling in response to different patterns of blue light stimulations.
The optoTGFBRs system was used to characterize TGF-β signaling dynamics in response to different patterns of blue light stimulation by monitoring subcellular localization of TGF-β receptor and Smad2 proteins.
By simultaneously monitoring the subcellular localization of TGF-β receptor and Smad2 proteins, we characterized the dynamics of TGF-β signaling in response to different patterns of blue light stimulations.
The optoTGFBRs system was used to characterize TGF-β signaling dynamics in response to different patterns of blue light stimulation by monitoring subcellular localization of TGF-β receptor and Smad2 proteins.
By simultaneously monitoring the subcellular localization of TGF-β receptor and Smad2 proteins, we characterized the dynamics of TGF-β signaling in response to different patterns of blue light stimulations.
The optoTGFBRs system was used to characterize TGF-β signaling dynamics in response to different patterns of blue light stimulation by monitoring subcellular localization of TGF-β receptor and Smad2 proteins.
By simultaneously monitoring the subcellular localization of TGF-β receptor and Smad2 proteins, we characterized the dynamics of TGF-β signaling in response to different patterns of blue light stimulations.
The authors developed an optogenetic system, optoTGFBRs, for precise control of TGF-β signaling in time and space.
Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-β signaling in time and space.
The authors developed an optogenetic system, optoTGFBRs, for precise control of TGF-β signaling in time and space.
Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-β signaling in time and space.
The authors developed an optogenetic system, optoTGFBRs, for precise control of TGF-β signaling in time and space.
Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-β signaling in time and space.
The authors developed an optogenetic system, optoTGFBRs, for precise control of TGF-β signaling in time and space.
Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-β signaling in time and space.
The authors developed an optogenetic system, optoTGFBRs, for precise control of TGF-β signaling in time and space.
Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-β signaling in time and space.
The authors developed an optogenetic system, optoTGFBRs, for precise control of TGF-β signaling in time and space.
Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-β signaling in time and space.
The authors developed an optogenetic system, optoTGFBRs, for precise control of TGF-β signaling in time and space.
Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-β signaling in time and space.
The spatial and temporal precision of light control makes optoTGFBRs a tool for quantitative analyses of TGF-β signaling at the single-cell level.
The spatial and temporal precision of light control will make the optoTGFBRs system as a powerful tool for quantitative analyses of TGF-β signaling at the single cell level.
The spatial and temporal precision of light control makes optoTGFBRs a tool for quantitative analyses of TGF-β signaling at the single-cell level.
The spatial and temporal precision of light control will make the optoTGFBRs system as a powerful tool for quantitative analyses of TGF-β signaling at the single cell level.
The spatial and temporal precision of light control makes optoTGFBRs a tool for quantitative analyses of TGF-β signaling at the single-cell level.
The spatial and temporal precision of light control will make the optoTGFBRs system as a powerful tool for quantitative analyses of TGF-β signaling at the single cell level.
The spatial and temporal precision of light control makes optoTGFBRs a tool for quantitative analyses of TGF-β signaling at the single-cell level.
The spatial and temporal precision of light control will make the optoTGFBRs system as a powerful tool for quantitative analyses of TGF-β signaling at the single cell level.
The spatial and temporal precision of light control makes optoTGFBRs a tool for quantitative analyses of TGF-β signaling at the single-cell level.
The spatial and temporal precision of light control will make the optoTGFBRs system as a powerful tool for quantitative analyses of TGF-β signaling at the single cell level.
The spatial and temporal precision of light control makes optoTGFBRs a tool for quantitative analyses of TGF-β signaling at the single-cell level.
The spatial and temporal precision of light control will make the optoTGFBRs system as a powerful tool for quantitative analyses of TGF-β signaling at the single cell level.
The spatial and temporal precision of light control makes optoTGFBRs a tool for quantitative analyses of TGF-β signaling at the single-cell level.
The spatial and temporal precision of light control will make the optoTGFBRs system as a powerful tool for quantitative analyses of TGF-β signaling at the single cell level.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug optotgfbrs
Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-β signaling in time and space.
Source:
functional capabilitysupports
Using optoTGFBRs, TGF-β signaling can be selectively and sequentially activated in single cells by modulating the pattern of light stimulations.
Using the optoTGFBRs system, we show that TGF-β signaling can be selectively and sequentially activated in single cells through the modulation of the pattern of light stimulations.
Source:
measurement capabilitysupports
The optoTGFBRs system was used to characterize TGF-β signaling dynamics in response to different patterns of blue light stimulation by monitoring subcellular localization of TGF-β receptor and Smad2 proteins.
By simultaneously monitoring the subcellular localization of TGF-β receptor and Smad2 proteins, we characterized the dynamics of TGF-β signaling in response to different patterns of blue light stimulations.
Source:
tool developmentsupports
The authors developed an optogenetic system, optoTGFBRs, for precise control of TGF-β signaling in time and space.
Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-β signaling in time and space.
Source:
use casesupports
The spatial and temporal precision of light control makes optoTGFBRs a tool for quantitative analyses of TGF-β signaling at the single-cell level.
The spatial and temporal precision of light control will make the optoTGFBRs system as a powerful tool for quantitative analyses of TGF-β signaling at the single cell level.
Source:
Comparisons
Source-backed strengths
The reported strengths are precise spatiotemporal control of TGF-β signaling and the ability to selectively and sequentially activate signaling in single cells by modulating light stimulation patterns. The system was also applied to characterize signaling dynamics through subcellular localization readouts of TGF-β receptor and Smad2.
Ranked Citations
- 1.