Source 1review2021Frontiers in Molecular Biosciences
Toolkit/orthogonal degrons
orthogonal degrons
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Orthogonal degrons are bacterial construct patterns used in tunable degradation systems to direct targeted proteolysis of proteins of interest. The cited literature places them among recent advances that enable large screens and functional interrogation through regulated protein degradation.
Usefulness & Problems
Why this is useful
These constructs are useful for interrogating and controlling protein function in bacteria through degradation rather than chemical genetics approaches. The source also discusses bacterial degraders more broadly as potential tools for protein-function studies and as candidate antimicrobial strategies.
Source:
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
Problem solved
Orthogonal degrons address the problem of how to perturb bacterial protein function in a regulated manner using targeted degradation. The cited review specifically associates them with tunable degradation systems and large-screen applications.
Problem links
Need better screening or enrichment leverage
DerivedOrthogonal degrons are construct patterns used in tunable bacterial degradation systems to control targeted proteolysis of proteins of interest. The cited literature places them within recent advances enabling large screens and functional interrogation based on regulated protein degradation.
Need conditional protein clearance
DerivedOrthogonal degrons are construct patterns used in tunable bacterial degradation systems to control targeted proteolysis of proteins of interest. The cited literature places them within recent advances enabling large screens and functional interrogation based on regulated protein degradation.
Need conditional recombination or state switching
DerivedOrthogonal degrons are construct patterns used in tunable bacterial degradation systems to control targeted proteolysis of proteins of interest. The cited literature places them within recent advances enabling large screens and functional interrogation based on regulated protein degradation.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
Degradationtargeted protein degradationtargeted protein degradationtunable proteolysistunable proteolysisTechniques
Functional AssayFunctional AssayFunctional AssaySelection / EnrichmentSelection / EnrichmentSelection / EnrichmentTarget processes
degradationrecombinationselectionImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: actuatoroperating role: sensor
Implementation is described only at the level of using orthogonal degrons within tunable bacterial degradation systems. The provided evidence does not report construct architecture, expression requirements, delivery methods, or cofactor dependencies.
The supplied evidence does not specify particular degron sequences, cognate proteases, host species, degradation kinetics, or quantitative performance. Independent experimental benchmarks and direct validation details for orthogonal degrons are not provided in the extracted text.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used instead of chemical genetics approaches to interrogate and control protein function.
In bacteria, degrons can be used in place of chemical genetics approaches to interrogate and control protein function.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Bacterial degraders are discussed as potential tools for studying protein function and as novel antimicrobials.
we discuss perspectives on using bacterial degraders for studying protein function and as novel antimicrobials.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
Recent advances include sophisticated tools and sensors for imaging based on bacterial targeted proteolysis concepts.
to sophisticated tools and sensors for imaging.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
The review covers synthetic applications of degrons in targeted proteolysis in bacteria.
Here, we provide a comprehensive review of synthetic applications of degrons in targeted proteolysis in bacteria.
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Approval Evidence
1 source1 linked approval claimfirst-pass slug orthogonal-degrons
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Source:
screening applicationsupports
Recent advances include large screens that employ tunable degradation systems and orthogonal degrons.
We describe recent advances ranging from large screens employing tunable degradation systems and orthogonal degrons
Source:
Comparisons
Source-backed strengths
The available evidence supports utility in tunable bacterial proteolysis, functional interrogation, and large screening formats. The literature also indicates that degron-based approaches can substitute for chemical genetics in some bacterial protein-function studies.
Compared with bacterial degrons
orthogonal degrons and bacterial degrons address a similar problem space because they share degradation.
Shared frame: same top-level item type; shared target processes: degradation; shared mechanisms: degradation
Compared with CRY2-lacZ gene fusion
orthogonal degrons and CRY2-lacZ gene fusion address a similar problem space because they share degradation, recombination.
Shared frame: same top-level item type; shared target processes: degradation, recombination; shared mechanisms: degradation
Compared with PCB synthesis expression vector
orthogonal degrons and PCB synthesis expression vector address a similar problem space because they share degradation, recombination.
Shared frame: same top-level item type; shared target processes: degradation, recombination; shared mechanisms: degradation
Strengths here: looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Ranked Citations
- 1.