Source 1primary paper2020
Toolkit/OXTR-iTango2
OXTR-iTango2
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
OXTR-iTango2 is a genetically encoded optogenetic gene-expression system designed to label neuronal populations activated by oxytocin under blue-light illumination. It supported quantitative reporter induction in HEK cells and neurons and was used to identify oxytocin-responsive neurons in vivo.
Usefulness & Problems
Why this is useful
This tool is useful for linking oxytocin receptor activation to durable reporter expression, enabling selective marking of oxytocin-sensitive cells rather than only transient signal detection. The cited study used it to detect ventral tegmental area dopamine neurons receiving oxytocin activation during social interaction.
Source:
In vivo expression of OXTR-iTango2 selectively labeled OXT-sensitive neurons in a blue-light dependent manner.
Source:
Thus, we provide a genetically-encoded, scalable optogenetic toolset to target neural circuits activated by OXT in behaving animals with a high temporal resolution.
Source:
Here we developed an optogenetic gene expression system designed to selectively label neuronal populations activated by OXT in the presence of blue-light, named “OXTR-iTango2”.
Problem solved
OXTR-iTango2 addresses the problem of selectively identifying and labeling neurons activated by oxytocin in complex tissues and behaving animals. The available evidence indicates that this labeling requires blue light and can be coupled to reporter gene induction.
Source:
Here we developed an optogenetic gene expression system designed to selectively label neuronal populations activated by OXT in the presence of blue-light, named “OXTR-iTango2”.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
Computational DesignTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The available evidence supports that OXTR-iTango2 is genetically encoded, functions as a multi-component optogenetic gene-expression system, and requires blue light for selective labeling. It was expressed in HEK cells, neurons, and in vivo, but the supplied evidence does not specify promoter architecture, cofactors, viral delivery, or component stoichiometry.
The supplied evidence does not provide molecular construct details, kinetic performance, background activity, spectral tuning, or comparative benchmarking against other activity-labeling systems. Independent replication is not provided in the cited material, and validation appears to come from a single 2020 source.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
OXTR-iTango2 induced reporter gene expression in HEK cells and neurons in a quantitative manner.
The OXTR-iTango2 was capable of inducing gene expression of a reporter gene in both human embryonic kidney (HEK) cells and neurons in a quantitative manner.
OXTR-iTango2 induced reporter gene expression in HEK cells and neurons in a quantitative manner.
The OXTR-iTango2 was capable of inducing gene expression of a reporter gene in both human embryonic kidney (HEK) cells and neurons in a quantitative manner.
OXTR-iTango2 induced reporter gene expression in HEK cells and neurons in a quantitative manner.
The OXTR-iTango2 was capable of inducing gene expression of a reporter gene in both human embryonic kidney (HEK) cells and neurons in a quantitative manner.
OXTR-iTango2 induced reporter gene expression in HEK cells and neurons in a quantitative manner.
The OXTR-iTango2 was capable of inducing gene expression of a reporter gene in both human embryonic kidney (HEK) cells and neurons in a quantitative manner.
OXTR-iTango2 induced reporter gene expression in HEK cells and neurons in a quantitative manner.
The OXTR-iTango2 was capable of inducing gene expression of a reporter gene in both human embryonic kidney (HEK) cells and neurons in a quantitative manner.
OXTR-iTango2 induced reporter gene expression in HEK cells and neurons in a quantitative manner.
The OXTR-iTango2 was capable of inducing gene expression of a reporter gene in both human embryonic kidney (HEK) cells and neurons in a quantitative manner.
OXTR-iTango2 induced reporter gene expression in HEK cells and neurons in a quantitative manner.
The OXTR-iTango2 was capable of inducing gene expression of a reporter gene in both human embryonic kidney (HEK) cells and neurons in a quantitative manner.
Using OXTR-iTango2, the authors detected a subset of ventral tegmental area dopamine neurons that receive oxytocin activation during social interaction.
Furthermore, we were able to detect a subset of dopamine (DA) neurons in the ventral tegmental area (VTA) that receive OXT activation during social interaction.
Using OXTR-iTango2, the authors detected a subset of ventral tegmental area dopamine neurons that receive oxytocin activation during social interaction.
Furthermore, we were able to detect a subset of dopamine (DA) neurons in the ventral tegmental area (VTA) that receive OXT activation during social interaction.
Using OXTR-iTango2, the authors detected a subset of ventral tegmental area dopamine neurons that receive oxytocin activation during social interaction.
Furthermore, we were able to detect a subset of dopamine (DA) neurons in the ventral tegmental area (VTA) that receive OXT activation during social interaction.
Using OXTR-iTango2, the authors detected a subset of ventral tegmental area dopamine neurons that receive oxytocin activation during social interaction.
Furthermore, we were able to detect a subset of dopamine (DA) neurons in the ventral tegmental area (VTA) that receive OXT activation during social interaction.
Using OXTR-iTango2, the authors detected a subset of ventral tegmental area dopamine neurons that receive oxytocin activation during social interaction.
Furthermore, we were able to detect a subset of dopamine (DA) neurons in the ventral tegmental area (VTA) that receive OXT activation during social interaction.
Using OXTR-iTango2, the authors detected a subset of ventral tegmental area dopamine neurons that receive oxytocin activation during social interaction.
Furthermore, we were able to detect a subset of dopamine (DA) neurons in the ventral tegmental area (VTA) that receive OXT activation during social interaction.
Using OXTR-iTango2, the authors detected a subset of ventral tegmental area dopamine neurons that receive oxytocin activation during social interaction.
Furthermore, we were able to detect a subset of dopamine (DA) neurons in the ventral tegmental area (VTA) that receive OXT activation during social interaction.
In vivo expression of OXTR-iTango2 selectively labeled oxytocin-sensitive neurons in a blue-light-dependent manner.
In vivo expression of OXTR-iTango2 selectively labeled OXT-sensitive neurons in a blue-light dependent manner.
In vivo expression of OXTR-iTango2 selectively labeled oxytocin-sensitive neurons in a blue-light-dependent manner.
In vivo expression of OXTR-iTango2 selectively labeled OXT-sensitive neurons in a blue-light dependent manner.
In vivo expression of OXTR-iTango2 selectively labeled oxytocin-sensitive neurons in a blue-light-dependent manner.
In vivo expression of OXTR-iTango2 selectively labeled OXT-sensitive neurons in a blue-light dependent manner.
In vivo expression of OXTR-iTango2 selectively labeled oxytocin-sensitive neurons in a blue-light-dependent manner.
In vivo expression of OXTR-iTango2 selectively labeled OXT-sensitive neurons in a blue-light dependent manner.
In vivo expression of OXTR-iTango2 selectively labeled oxytocin-sensitive neurons in a blue-light-dependent manner.
In vivo expression of OXTR-iTango2 selectively labeled OXT-sensitive neurons in a blue-light dependent manner.
In vivo expression of OXTR-iTango2 selectively labeled oxytocin-sensitive neurons in a blue-light-dependent manner.
In vivo expression of OXTR-iTango2 selectively labeled OXT-sensitive neurons in a blue-light dependent manner.
In vivo expression of OXTR-iTango2 selectively labeled oxytocin-sensitive neurons in a blue-light-dependent manner.
In vivo expression of OXTR-iTango2 selectively labeled OXT-sensitive neurons in a blue-light dependent manner.
The study presents a genetically encoded, scalable optogenetic toolset for targeting neural circuits activated by oxytocin in behaving animals with high temporal resolution.
Thus, we provide a genetically-encoded, scalable optogenetic toolset to target neural circuits activated by OXT in behaving animals with a high temporal resolution.
The study presents a genetically encoded, scalable optogenetic toolset for targeting neural circuits activated by oxytocin in behaving animals with high temporal resolution.
Thus, we provide a genetically-encoded, scalable optogenetic toolset to target neural circuits activated by OXT in behaving animals with a high temporal resolution.
The study presents a genetically encoded, scalable optogenetic toolset for targeting neural circuits activated by oxytocin in behaving animals with high temporal resolution.
Thus, we provide a genetically-encoded, scalable optogenetic toolset to target neural circuits activated by OXT in behaving animals with a high temporal resolution.
The study presents a genetically encoded, scalable optogenetic toolset for targeting neural circuits activated by oxytocin in behaving animals with high temporal resolution.
Thus, we provide a genetically-encoded, scalable optogenetic toolset to target neural circuits activated by OXT in behaving animals with a high temporal resolution.
The study presents a genetically encoded, scalable optogenetic toolset for targeting neural circuits activated by oxytocin in behaving animals with high temporal resolution.
Thus, we provide a genetically-encoded, scalable optogenetic toolset to target neural circuits activated by OXT in behaving animals with a high temporal resolution.
The study presents a genetically encoded, scalable optogenetic toolset for targeting neural circuits activated by oxytocin in behaving animals with high temporal resolution.
Thus, we provide a genetically-encoded, scalable optogenetic toolset to target neural circuits activated by OXT in behaving animals with a high temporal resolution.
The study presents a genetically encoded, scalable optogenetic toolset for targeting neural circuits activated by oxytocin in behaving animals with high temporal resolution.
Thus, we provide a genetically-encoded, scalable optogenetic toolset to target neural circuits activated by OXT in behaving animals with a high temporal resolution.
The authors developed OXTR-iTango2, an optogenetic gene expression system designed to selectively label neuronal populations activated by oxytocin in the presence of blue light.
Here we developed an optogenetic gene expression system designed to selectively label neuronal populations activated by OXT in the presence of blue-light, named “OXTR-iTango2”.
The authors developed OXTR-iTango2, an optogenetic gene expression system designed to selectively label neuronal populations activated by oxytocin in the presence of blue light.
Here we developed an optogenetic gene expression system designed to selectively label neuronal populations activated by OXT in the presence of blue-light, named “OXTR-iTango2”.
The authors developed OXTR-iTango2, an optogenetic gene expression system designed to selectively label neuronal populations activated by oxytocin in the presence of blue light.
Here we developed an optogenetic gene expression system designed to selectively label neuronal populations activated by OXT in the presence of blue-light, named “OXTR-iTango2”.
The authors developed OXTR-iTango2, an optogenetic gene expression system designed to selectively label neuronal populations activated by oxytocin in the presence of blue light.
Here we developed an optogenetic gene expression system designed to selectively label neuronal populations activated by OXT in the presence of blue-light, named “OXTR-iTango2”.
The authors developed OXTR-iTango2, an optogenetic gene expression system designed to selectively label neuronal populations activated by oxytocin in the presence of blue light.
Here we developed an optogenetic gene expression system designed to selectively label neuronal populations activated by OXT in the presence of blue-light, named “OXTR-iTango2”.
The authors developed OXTR-iTango2, an optogenetic gene expression system designed to selectively label neuronal populations activated by oxytocin in the presence of blue light.
Here we developed an optogenetic gene expression system designed to selectively label neuronal populations activated by OXT in the presence of blue-light, named “OXTR-iTango2”.
The authors developed OXTR-iTango2, an optogenetic gene expression system designed to selectively label neuronal populations activated by oxytocin in the presence of blue light.
Here we developed an optogenetic gene expression system designed to selectively label neuronal populations activated by OXT in the presence of blue-light, named “OXTR-iTango2”.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug oxtr-itango2
Here we developed an optogenetic gene expression system designed to selectively label neuronal populations activated by OXT in the presence of blue-light, named “OXTR-iTango2”.
Source:
cellular activitysupports
OXTR-iTango2 induced reporter gene expression in HEK cells and neurons in a quantitative manner.
The OXTR-iTango2 was capable of inducing gene expression of a reporter gene in both human embryonic kidney (HEK) cells and neurons in a quantitative manner.
Source:
circuit detectionsupports
Using OXTR-iTango2, the authors detected a subset of ventral tegmental area dopamine neurons that receive oxytocin activation during social interaction.
Furthermore, we were able to detect a subset of dopamine (DA) neurons in the ventral tegmental area (VTA) that receive OXT activation during social interaction.
Source:
in vivo labelingsupports
In vivo expression of OXTR-iTango2 selectively labeled oxytocin-sensitive neurons in a blue-light-dependent manner.
In vivo expression of OXTR-iTango2 selectively labeled OXT-sensitive neurons in a blue-light dependent manner.
Source:
tool capabilitysupports
The study presents a genetically encoded, scalable optogenetic toolset for targeting neural circuits activated by oxytocin in behaving animals with high temporal resolution.
Thus, we provide a genetically-encoded, scalable optogenetic toolset to target neural circuits activated by OXT in behaving animals with a high temporal resolution.
Source:
tool developmentsupports
The authors developed OXTR-iTango2, an optogenetic gene expression system designed to selectively label neuronal populations activated by oxytocin in the presence of blue light.
Here we developed an optogenetic gene expression system designed to selectively label neuronal populations activated by OXT in the presence of blue-light, named “OXTR-iTango2”.
Source:
Comparisons
Source-backed strengths
The reported strengths are quantitative reporter gene expression in both HEK cells and neurons and selective in vivo labeling of oxytocin-sensitive neurons in a blue-light-dependent manner. It was also demonstrated in a circuit-mapping context by detecting a subset of ventral tegmental area dopamine neurons activated by oxytocin during social interaction.
Ranked Citations
- 1.