Source 1primary paper2023Biology
Toolkit/p21-CRY2/CIB1
p21-CRY2/CIB1
Also known as: cryptochrome 2/CIBN p21 fusion system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
p21-CRY2/CIB1 is a blue-light-responsive optogenetic p21 system built by fusing p21 to the cryptochrome 2/CIBN switch components. It was used to control p21 subcellular localization and nuclear function and increased the fraction of cells arrested in G1 phase.
Usefulness & Problems
Why this is useful
This system provides light-dependent control over the localization-dependent activity of the cyclin-dependent kinase inhibitor p21. The cited study further proposed its application in producer cell lines to uncouple proliferation from G1 arrest for biotherapeutic protein production.
Source:
Light-controllable p21 implemented in producer cell lines could be applied to steer the uncoupling of cell proliferation and cell cycle arrest at the G1 phase optimizing the production of biotherapeutic proteins.
Source:
Here, we applied an optogenetic approach to control the subcellular localization of p21 and its nuclear functions.
Problem solved
It addresses the need for externally controllable regulation of p21 function, specifically its subcellular localization and nuclear activity. The reported implementation also targets controlled induction of G1 cell cycle arrest.
Source:
Light-controllable p21 implemented in producer cell lines could be applied to steer the uncoupling of cell proliferation and cell cycle arrest at the G1 phase optimizing the production of biotherapeutic proteins.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
light-induced protein heterodimerizationmembrane recruitmentMembrane Recruitmentsubcellular relocalizationTechniques
No technique tags yet.
Target processes
localizationrecombinationInput: Light
Implementation Constraints
The system was generated through fusions of p21 with the blue-light switch cryptochrome 2/CIBN, indicating a multi-component construct design. The provided evidence does not specify exact fusion topology, expression system, light dose, or any required cofactors.
The supplied evidence is limited to a single 2023 study and does not provide quantitative performance metrics, kinetics, reversibility, or cell-type breadth. Practical details of construct architecture, illumination parameters, and comparative performance against other optogenetic p21 designs are not given in the provided evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Light-controllable p21 in producer cell lines could be applied to uncouple cell proliferation from G1 cell cycle arrest to optimize biotherapeutic protein production.
Light-controllable p21 implemented in producer cell lines could be applied to steer the uncoupling of cell proliferation and cell cycle arrest at the G1 phase optimizing the production of biotherapeutic proteins.
Light-controllable p21 in producer cell lines could be applied to uncouple cell proliferation from G1 cell cycle arrest to optimize biotherapeutic protein production.
Light-controllable p21 implemented in producer cell lines could be applied to steer the uncoupling of cell proliferation and cell cycle arrest at the G1 phase optimizing the production of biotherapeutic proteins.
Light-controllable p21 in producer cell lines could be applied to uncouple cell proliferation from G1 cell cycle arrest to optimize biotherapeutic protein production.
Light-controllable p21 implemented in producer cell lines could be applied to steer the uncoupling of cell proliferation and cell cycle arrest at the G1 phase optimizing the production of biotherapeutic proteins.
Light-controllable p21 in producer cell lines could be applied to uncouple cell proliferation from G1 cell cycle arrest to optimize biotherapeutic protein production.
Light-controllable p21 implemented in producer cell lines could be applied to steer the uncoupling of cell proliferation and cell cycle arrest at the G1 phase optimizing the production of biotherapeutic proteins.
Light-controllable p21 in producer cell lines could be applied to uncouple cell proliferation from G1 cell cycle arrest to optimize biotherapeutic protein production.
Light-controllable p21 implemented in producer cell lines could be applied to steer the uncoupling of cell proliferation and cell cycle arrest at the G1 phase optimizing the production of biotherapeutic proteins.
Light-controllable p21 in producer cell lines could be applied to uncouple cell proliferation from G1 cell cycle arrest to optimize biotherapeutic protein production.
Light-controllable p21 implemented in producer cell lines could be applied to steer the uncoupling of cell proliferation and cell cycle arrest at the G1 phase optimizing the production of biotherapeutic proteins.
Light-controllable p21 in producer cell lines could be applied to uncouple cell proliferation from G1 cell cycle arrest to optimize biotherapeutic protein production.
Light-controllable p21 implemented in producer cell lines could be applied to steer the uncoupling of cell proliferation and cell cycle arrest at the G1 phase optimizing the production of biotherapeutic proteins.
Both p21-CRY2/CIB1 and p21-LINuS increased the amount of cells arrested in the G1 phase.
Both systems, p21-CRY2/CIB1 and p21-LINuS, increased the amounts of cells arrested in the G1 phase
Both p21-CRY2/CIB1 and p21-LINuS increased the amount of cells arrested in the G1 phase.
Both systems, p21-CRY2/CIB1 and p21-LINuS, increased the amounts of cells arrested in the G1 phase
Both p21-CRY2/CIB1 and p21-LINuS increased the amount of cells arrested in the G1 phase.
Both systems, p21-CRY2/CIB1 and p21-LINuS, increased the amounts of cells arrested in the G1 phase
Both p21-CRY2/CIB1 and p21-LINuS increased the amount of cells arrested in the G1 phase.
Both systems, p21-CRY2/CIB1 and p21-LINuS, increased the amounts of cells arrested in the G1 phase
Both p21-CRY2/CIB1 and p21-LINuS increased the amount of cells arrested in the G1 phase.
Both systems, p21-CRY2/CIB1 and p21-LINuS, increased the amounts of cells arrested in the G1 phase
Both p21-CRY2/CIB1 and p21-LINuS increased the amount of cells arrested in the G1 phase.
Both systems, p21-CRY2/CIB1 and p21-LINuS, increased the amounts of cells arrested in the G1 phase
Both p21-CRY2/CIB1 and p21-LINuS increased the amount of cells arrested in the G1 phase.
Both systems, p21-CRY2/CIB1 and p21-LINuS, increased the amounts of cells arrested in the G1 phase
Optogenetically controlled p21 systems were used to control the subcellular localization and nuclear functions of p21.
Here, we applied an optogenetic approach to control the subcellular localization of p21 and its nuclear functions.
Optogenetically controlled p21 systems were used to control the subcellular localization and nuclear functions of p21.
Here, we applied an optogenetic approach to control the subcellular localization of p21 and its nuclear functions.
Optogenetically controlled p21 systems were used to control the subcellular localization and nuclear functions of p21.
Here, we applied an optogenetic approach to control the subcellular localization of p21 and its nuclear functions.
Optogenetically controlled p21 systems were used to control the subcellular localization and nuclear functions of p21.
Here, we applied an optogenetic approach to control the subcellular localization of p21 and its nuclear functions.
Optogenetically controlled p21 systems were used to control the subcellular localization and nuclear functions of p21.
Here, we applied an optogenetic approach to control the subcellular localization of p21 and its nuclear functions.
Optogenetically controlled p21 systems were used to control the subcellular localization and nuclear functions of p21.
Here, we applied an optogenetic approach to control the subcellular localization of p21 and its nuclear functions.
Optogenetically controlled p21 systems were used to control the subcellular localization and nuclear functions of p21.
Here, we applied an optogenetic approach to control the subcellular localization of p21 and its nuclear functions.
The increase in G1-arrested cells correlated with increased cell-specific productivity of secreted alkaline phosphatase.
Both systems, p21-CRY2/CIB1 and p21-LINuS, increased the amounts of cells arrested in the G1 phase correlating with the increased cell-specific productivity of the reporter-protein-secreted alkaline phosphatase.
The increase in G1-arrested cells correlated with increased cell-specific productivity of secreted alkaline phosphatase.
Both systems, p21-CRY2/CIB1 and p21-LINuS, increased the amounts of cells arrested in the G1 phase correlating with the increased cell-specific productivity of the reporter-protein-secreted alkaline phosphatase.
The increase in G1-arrested cells correlated with increased cell-specific productivity of secreted alkaline phosphatase.
Both systems, p21-CRY2/CIB1 and p21-LINuS, increased the amounts of cells arrested in the G1 phase correlating with the increased cell-specific productivity of the reporter-protein-secreted alkaline phosphatase.
The increase in G1-arrested cells correlated with increased cell-specific productivity of secreted alkaline phosphatase.
Both systems, p21-CRY2/CIB1 and p21-LINuS, increased the amounts of cells arrested in the G1 phase correlating with the increased cell-specific productivity of the reporter-protein-secreted alkaline phosphatase.
The increase in G1-arrested cells correlated with increased cell-specific productivity of secreted alkaline phosphatase.
Both systems, p21-CRY2/CIB1 and p21-LINuS, increased the amounts of cells arrested in the G1 phase correlating with the increased cell-specific productivity of the reporter-protein-secreted alkaline phosphatase.
The increase in G1-arrested cells correlated with increased cell-specific productivity of secreted alkaline phosphatase.
Both systems, p21-CRY2/CIB1 and p21-LINuS, increased the amounts of cells arrested in the G1 phase correlating with the increased cell-specific productivity of the reporter-protein-secreted alkaline phosphatase.
The increase in G1-arrested cells correlated with increased cell-specific productivity of secreted alkaline phosphatase.
Both systems, p21-CRY2/CIB1 and p21-LINuS, increased the amounts of cells arrested in the G1 phase correlating with the increased cell-specific productivity of the reporter-protein-secreted alkaline phosphatase.
Blue LED exposure interval and light dose enabled fine-tuning of the optogenetic p21 systems.
Varying the intervals of blue LED light exposure and the light dose enable the fine-tuning of the systems.
Blue LED exposure interval and light dose enabled fine-tuning of the optogenetic p21 systems.
Varying the intervals of blue LED light exposure and the light dose enable the fine-tuning of the systems.
Blue LED exposure interval and light dose enabled fine-tuning of the optogenetic p21 systems.
Varying the intervals of blue LED light exposure and the light dose enable the fine-tuning of the systems.
Blue LED exposure interval and light dose enabled fine-tuning of the optogenetic p21 systems.
Varying the intervals of blue LED light exposure and the light dose enable the fine-tuning of the systems.
Blue LED exposure interval and light dose enabled fine-tuning of the optogenetic p21 systems.
Varying the intervals of blue LED light exposure and the light dose enable the fine-tuning of the systems.
Blue LED exposure interval and light dose enabled fine-tuning of the optogenetic p21 systems.
Varying the intervals of blue LED light exposure and the light dose enable the fine-tuning of the systems.
Blue LED exposure interval and light dose enabled fine-tuning of the optogenetic p21 systems.
Varying the intervals of blue LED light exposure and the light dose enable the fine-tuning of the systems.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug p21-cry2-cib1
To generate light-controllable p21, appropriate fusions with the blue light switch cryptochrome 2/CIBN ... were used. Both systems, p21-CRY2/CIB1 and p21-LINuS...
Source:
application potentialsupports
Light-controllable p21 in producer cell lines could be applied to uncouple cell proliferation from G1 cell cycle arrest to optimize biotherapeutic protein production.
Light-controllable p21 implemented in producer cell lines could be applied to steer the uncoupling of cell proliferation and cell cycle arrest at the G1 phase optimizing the production of biotherapeutic proteins.
Source:
cell cycle arrestsupports
Both p21-CRY2/CIB1 and p21-LINuS increased the amount of cells arrested in the G1 phase.
Both systems, p21-CRY2/CIB1 and p21-LINuS, increased the amounts of cells arrested in the G1 phase
Source:
functional effectsupports
Optogenetically controlled p21 systems were used to control the subcellular localization and nuclear functions of p21.
Here, we applied an optogenetic approach to control the subcellular localization of p21 and its nuclear functions.
Source:
productivity effectsupports
The increase in G1-arrested cells correlated with increased cell-specific productivity of secreted alkaline phosphatase.
Both systems, p21-CRY2/CIB1 and p21-LINuS, increased the amounts of cells arrested in the G1 phase correlating with the increased cell-specific productivity of the reporter-protein-secreted alkaline phosphatase.
Source:
tunabilitysupports
Blue LED exposure interval and light dose enabled fine-tuning of the optogenetic p21 systems.
Varying the intervals of blue LED light exposure and the light dose enable the fine-tuning of the systems.
Source:
Comparisons
Source-backed strengths
The tool is responsive to blue light and was experimentally used to regulate p21 localization and nuclear functions. In the cited work, p21-CRY2/CIB1 increased the proportion of cells arrested in G1 phase.
Ranked Citations
- 1.