Source 1primary paper2025MED
Toolkit/P3 editing
P3 editing
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Here, we demonstrate a strategy termed 'P3 editing', which links protein-protein proximity to the formation of a functional CRISPR-Cas9 dual-component guide RNA.
Usefulness & Problems
No literature-backed usefulness or problem-fit explainer has been materialized for this record yet.
Published Workflows
Objective: Engineer a molecular circuit input layer in which protein-protein proximity triggers CRISPR-based genome editing through conditional formation of a functional dual-component guide RNA.
Why it works: The strategy is expected to work because protein-protein proximity is converted into formation of a functional CRISPR-Cas9 dual-component guide RNA by engineering the crRNA:tracrRNA interaction.
protein-protein proximity-dependent guide RNA assemblyengineered crRNA:tracrRNA interactionRNA-sensor-to-genome-editing signal couplingguide RNA engineeringprime editingbase editing
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
editingInput: Chemical
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successMammalian Cell Lineapplication demohuman
Inferred from claim c3 during normalization. Known protein-protein interactions and chemically induced dimerization of protein domains can be used with P3 editing to activate prime editing or base editing in human cells. Derived from claim c3. Quoted text: we demonstrate that various known protein-protein interactions, as well as the chemically induced dimerization of protein domains, can be used to activate prime editing or base editing in human cells
Source:
mixedMammalian Cell Lineapplication demo
Inferred from claim c4 during normalization. P3 editing can incorporate outputs from ADAR-based RNA sensors, potentially allowing specific RNAs to induce specific genome edits within a larger circuit. Derived from claim c4. Quoted text: Additionally, we explore how P3 editing can incorporate outputs from ADAR-based RNA sensors, potentially allowing specific RNAs to induce specific genome edits within a larger circuit.
Source:
Supporting Sources
Ranked Claims
P3 editing enhances the controllability of CRISPR-based genome editing and facilitates its use in synthetic molecular circuits deployed in living cells.
Our strategy enhances the controllability of CRISPR-based genome editing, facilitating its use in synthetic molecular circuits deployed in living cells.
Known protein-protein interactions and chemically induced dimerization of protein domains can be used with P3 editing to activate prime editing or base editing in human cells.
we demonstrate that various known protein-protein interactions, as well as the chemically induced dimerization of protein domains, can be used to activate prime editing or base editing in human cells
P3 editing can incorporate outputs from ADAR-based RNA sensors, potentially allowing specific RNAs to induce specific genome edits within a larger circuit.
Additionally, we explore how P3 editing can incorporate outputs from ADAR-based RNA sensors, potentially allowing specific RNAs to induce specific genome edits within a larger circuit.
P3 editing is enabled by engineering the crRNA:tracrRNA interaction.
By engineering the crRNA:tracrRNA interaction
P3 editing links protein-protein proximity to formation of a functional CRISPR-Cas9 dual-component guide RNA.
Here, we demonstrate a strategy termed 'P3 editing', which links protein-protein proximity to the formation of a functional CRISPR-Cas9 dual-component guide RNA.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug p3-editing
Here, we demonstrate a strategy termed 'P3 editing', which links protein-protein proximity to the formation of a functional CRISPR-Cas9 dual-component guide RNA.
Source:
advantagesupports
P3 editing enhances the controllability of CRISPR-based genome editing and facilitates its use in synthetic molecular circuits deployed in living cells.
Our strategy enhances the controllability of CRISPR-based genome editing, facilitating its use in synthetic molecular circuits deployed in living cells.
Source:
application scopesupports
Known protein-protein interactions and chemically induced dimerization of protein domains can be used with P3 editing to activate prime editing or base editing in human cells.
we demonstrate that various known protein-protein interactions, as well as the chemically induced dimerization of protein domains, can be used to activate prime editing or base editing in human cells
Source:
integration capabilitysupports
P3 editing can incorporate outputs from ADAR-based RNA sensors, potentially allowing specific RNAs to induce specific genome edits within a larger circuit.
Additionally, we explore how P3 editing can incorporate outputs from ADAR-based RNA sensors, potentially allowing specific RNAs to induce specific genome edits within a larger circuit.
Source:
mechanismsupports
P3 editing is enabled by engineering the crRNA:tracrRNA interaction.
By engineering the crRNA:tracrRNA interaction
Source:
method capabilitysupports
P3 editing links protein-protein proximity to formation of a functional CRISPR-Cas9 dual-component guide RNA.
Here, we demonstrate a strategy termed 'P3 editing', which links protein-protein proximity to the formation of a functional CRISPR-Cas9 dual-component guide RNA.
Source:
Comparisons
No literature-backed comparison notes have been materialized for this record yet.
Ranked Citations
- 1.