Source 1primary paper2020Nature Communications
Toolkit/PA-Cre 3.0
PA-Cre 3.0
Also known as: PA Cre 3.0, photoactivatable Cre recombinase 3.0, photoactivatable (PA) Cre recombinase
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
PA-Cre 3.0 is a genetically encoded photoactivatable Cre recombinase system for light-controlled recombination in mammalian cells and in vivo mouse applications. It is an updated multi-component switch engineered to reduce background recombination and improve blue-light-induced Cre activity.
Usefulness & Problems
Why this is useful
PA-Cre 3.0 is useful for temporally controlling Cre-lox recombination with light while reducing unwanted recombination in the dark. The reported optimization for mammalian expression and in vivo mouse applications supports its use where low basal activity and inducible blue-light activation are important.
Source:
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
Source:
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
Problem solved
This tool addresses background recombination in earlier photoactivatable Cre designs, which was attributed to high sequence similarity in the dimerization domains. It also addresses the need for improved blue-light-induced recombination performance in mammalian and mouse contexts.
Source:
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
Source:
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
recombinationInput: Light
Implementation Constraints
The reported construct design includes mammalian expression optimization with a CAG promoter, Magnets, and a 2A self-cleaving peptide. Codon modifications were introduced for mouse gene targeting and viral production, indicating attention to sequence design for in vivo and vector-based implementation.
The supplied evidence does not provide quantitative performance metrics, wavelength details beyond blue light, or direct comparisons across multiple cell types or tissues. Independent replication is not documented in the provided evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Codon modifications for mouse gene targeting and viral production were introduced to prevent background recombination caused by high sequence similarity in the dimerization domains.
To prevent background recombination caused by the high sequence similarity in the dimerization domains, we modify the codons for mouse gene targeting and viral production.
Codon modifications for mouse gene targeting and viral production were introduced to prevent background recombination caused by high sequence similarity in the dimerization domains.
To prevent background recombination caused by the high sequence similarity in the dimerization domains, we modify the codons for mouse gene targeting and viral production.
Codon modifications for mouse gene targeting and viral production were introduced to prevent background recombination caused by high sequence similarity in the dimerization domains.
To prevent background recombination caused by the high sequence similarity in the dimerization domains, we modify the codons for mouse gene targeting and viral production.
Codon modifications for mouse gene targeting and viral production were introduced to prevent background recombination caused by high sequence similarity in the dimerization domains.
To prevent background recombination caused by the high sequence similarity in the dimerization domains, we modify the codons for mouse gene targeting and viral production.
Codon modifications for mouse gene targeting and viral production were introduced to prevent background recombination caused by high sequence similarity in the dimerization domains.
To prevent background recombination caused by the high sequence similarity in the dimerization domains, we modify the codons for mouse gene targeting and viral production.
Codon modifications for mouse gene targeting and viral production were introduced to prevent background recombination caused by high sequence similarity in the dimerization domains.
To prevent background recombination caused by the high sequence similarity in the dimerization domains, we modify the codons for mouse gene targeting and viral production.
Codon modifications for mouse gene targeting and viral production were introduced to prevent background recombination caused by high sequence similarity in the dimerization domains.
To prevent background recombination caused by the high sequence similarity in the dimerization domains, we modify the codons for mouse gene targeting and viral production.
PA-Cre 3.0 was optimized for mammalian expression using a CAG promoter, Magnets, and a 2A self-cleaving peptide.
To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide.
PA-Cre 3.0 was optimized for mammalian expression using a CAG promoter, Magnets, and a 2A self-cleaving peptide.
To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide.
PA-Cre 3.0 was optimized for mammalian expression using a CAG promoter, Magnets, and a 2A self-cleaving peptide.
To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide.
PA-Cre 3.0 was optimized for mammalian expression using a CAG promoter, Magnets, and a 2A self-cleaving peptide.
To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide.
PA-Cre 3.0 was optimized for mammalian expression using a CAG promoter, Magnets, and a 2A self-cleaving peptide.
To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide.
PA-Cre 3.0 was optimized for mammalian expression using a CAG promoter, Magnets, and a 2A self-cleaving peptide.
To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide.
PA-Cre 3.0 was optimized for mammalian expression using a CAG promoter, Magnets, and a 2A self-cleaving peptide.
To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide.
The reported modifications significantly reduce dark leak activity and improve blue-light induction in PA-Cre 3.0.
Overall, these modifications significantly reduce dark leak activity and improve blue-light induction developing our new version, PA-Cre 3.0.
The reported modifications significantly reduce dark leak activity and improve blue-light induction in PA-Cre 3.0.
Overall, these modifications significantly reduce dark leak activity and improve blue-light induction developing our new version, PA-Cre 3.0.
The reported modifications significantly reduce dark leak activity and improve blue-light induction in PA-Cre 3.0.
Overall, these modifications significantly reduce dark leak activity and improve blue-light induction developing our new version, PA-Cre 3.0.
The reported modifications significantly reduce dark leak activity and improve blue-light induction in PA-Cre 3.0.
Overall, these modifications significantly reduce dark leak activity and improve blue-light induction developing our new version, PA-Cre 3.0.
The reported modifications significantly reduce dark leak activity and improve blue-light induction in PA-Cre 3.0.
Overall, these modifications significantly reduce dark leak activity and improve blue-light induction developing our new version, PA-Cre 3.0.
The reported modifications significantly reduce dark leak activity and improve blue-light induction in PA-Cre 3.0.
Overall, these modifications significantly reduce dark leak activity and improve blue-light induction developing our new version, PA-Cre 3.0.
The reported modifications significantly reduce dark leak activity and improve blue-light induction in PA-Cre 3.0.
Overall, these modifications significantly reduce dark leak activity and improve blue-light induction developing our new version, PA-Cre 3.0.
The authors generated and validated AAV-PA-Cre 3.0 and two mouse lines that can conditionally express PA-Cre 3.0.
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
The authors generated and validated AAV-PA-Cre 3.0 and two mouse lines that can conditionally express PA-Cre 3.0.
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
The authors generated and validated AAV-PA-Cre 3.0 and two mouse lines that can conditionally express PA-Cre 3.0.
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
The authors generated and validated AAV-PA-Cre 3.0 and two mouse lines that can conditionally express PA-Cre 3.0.
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
The authors generated and validated AAV-PA-Cre 3.0 and two mouse lines that can conditionally express PA-Cre 3.0.
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
The authors generated and validated AAV-PA-Cre 3.0 and two mouse lines that can conditionally express PA-Cre 3.0.
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
The authors generated and validated AAV-PA-Cre 3.0 and two mouse lines that can conditionally express PA-Cre 3.0.
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
This paper reports a new version of photoactivatable Cre recombinase called PA-Cre 3.0.
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
This paper reports a new version of photoactivatable Cre recombinase called PA-Cre 3.0.
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
This paper reports a new version of photoactivatable Cre recombinase called PA-Cre 3.0.
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
This paper reports a new version of photoactivatable Cre recombinase called PA-Cre 3.0.
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
This paper reports a new version of photoactivatable Cre recombinase called PA-Cre 3.0.
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
This paper reports a new version of photoactivatable Cre recombinase called PA-Cre 3.0.
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
This paper reports a new version of photoactivatable Cre recombinase called PA-Cre 3.0.
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug pa-cre-3-0
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
Source:
background reductionsupports
Codon modifications for mouse gene targeting and viral production were introduced to prevent background recombination caused by high sequence similarity in the dimerization domains.
To prevent background recombination caused by the high sequence similarity in the dimerization domains, we modify the codons for mouse gene targeting and viral production.
Source:
engineering optimizationsupports
PA-Cre 3.0 was optimized for mammalian expression using a CAG promoter, Magnets, and a 2A self-cleaving peptide.
To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide.
Source:
performance improvementsupports
The reported modifications significantly reduce dark leak activity and improve blue-light induction in PA-Cre 3.0.
Overall, these modifications significantly reduce dark leak activity and improve blue-light induction developing our new version, PA-Cre 3.0.
Source:
resource generationsupports
The authors generated and validated AAV-PA-Cre 3.0 and two mouse lines that can conditionally express PA-Cre 3.0.
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
Source:
tool developmentsupports
This paper reports a new version of photoactivatable Cre recombinase called PA-Cre 3.0.
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
Source:
Comparisons
Source-backed strengths
The reported modifications significantly reduce dark leak activity and improve blue-light induction relative to prior versions. The system was specifically optimized for mammalian expression using a CAG promoter, Magnets, and a 2A self-cleaving peptide, and codon modifications were introduced for mouse gene targeting and viral production.
Source:
To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide.
Source:
Overall, these modifications significantly reduce dark leak activity and improve blue-light induction developing our new version, PA-Cre 3.0.
Ranked Citations
- 1.