Source 1primary paper2019Nucleic Acids Research
Toolkit/PA-Cre2.0
PA-Cre2.0
Also known as: photoactivatable Cre recombinase gene switch, split Cre recombinase
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
PA-Cre2.0 is a photoactivatable split Cre recombinase in which Cre activity is reconstituted by light-induced CRY2-CIB1 dimerization. It enables light-dependent control of Cre-mediated recombination and has been functionally characterized in mammalian cells and rodent brain.
Usefulness & Problems
Why this is useful
PA-Cre2.0 provides optical control over Cre-lox recombination, allowing recombinase activity to be restricted to illuminated conditions. The reported low background and sensitivity to brief light inputs in rodent brain support its use where tight temporal control and reduced basal recombination are important.
Source:
while in vivo the system also shows low background and sensitive response to brief light inputs
Problem solved
This tool addresses the problem of achieving tight control over split Cre recombinase activity so that recombination occurs in response to light rather than constitutively. The associated study also frames it as a platform for engineering and applying split protein fragments with improved control.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
Heterodimerizationlight-induced heterodimerizationsplit-protein reconstitutionsubcellular compartmentalization/localization controlTechniques
No technique tags yet.
Target processes
localizationrecombinationInput: Light
Implementation Constraints
PA-Cre2.0 is implemented as a split Cre recombinase whose fragments are reconstituted through fusion to the light-responsive CRY2-CIB1 dimerization pair. The evidence supports use in mammalian cells and rodent brain, but the supplied material does not specify construct architecture, promoters, delivery method, or exact light wavelength.
The supplied evidence does not provide quantitative performance metrics, illumination parameters, or direct comparisons to alternative photoactivatable Cre systems. Independent replication is not documented in the provided material, and validation is only explicitly stated for mammalian cells and rodent brain.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.
This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.
This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.
This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.
This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.
This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.
This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.
This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.
while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.
while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.
while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.
while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.
while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.
while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.
while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.
We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.
We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.
We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.
We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.
We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.
We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.
We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.
The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.
The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.
The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.
The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.
The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.
The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.
The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.
In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.
In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.
In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.
In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.
In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.
In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.
In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
Approval Evidence
1 source5 linked approval claimsfirst-pass slug pa-cre2-0
We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Source:
general conclusionsupports
This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.
This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Source:
in vivo performancesupports
In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.
while in vivo the system also shows low background and sensitive response to brief light inputs
Source:
mechanismsupports
PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.
We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Source:
mechanistic explanationsupports
The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.
The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Source:
performancesupports
In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.
In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Source:
Comparisons
Source-backed strengths
The literature reports in vivo functionality of PA-Cre2.0 in rodent brain. It is described as having low background activity and a sensitive response to brief light inputs, indicating favorable switching behavior for optical induction of recombination.
Source:
In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Ranked Citations
- 1.