Toolkit/PA-Cre2.0

PA-Cre2.0

Multi-Component Switch·Research·Since 2019

Also known as: photoactivatable Cre recombinase gene switch, split Cre recombinase

Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

PA-Cre2.0 is a photoactivatable split Cre recombinase in which Cre activity is reconstituted by light-induced CRY2-CIB1 dimerization. It enables light-dependent control of Cre-mediated recombination and has been functionally characterized in mammalian cells and rodent brain.

Usefulness & Problems

Why this is useful

PA-Cre2.0 provides optical control over Cre-lox recombination, allowing recombinase activity to be restricted to illuminated conditions. The reported low background and sensitivity to brief light inputs in rodent brain support its use where tight temporal control and reduced basal recombination are important.

Source:

while in vivo the system also shows low background and sensitive response to brief light inputs

Problem solved

This tool addresses the problem of achieving tight control over split Cre recombinase activity so that recombination occurs in response to light rather than constitutively. The associated study also frames it as a platform for engineering and applying split protein fragments with improved control.

Problem links

Need conditional recombination or state switching

Derived

PA-Cre2.0 is a photoactivatable split Cre recombinase gene switch in which Cre activity is reconstituted by light-induced CRY2-CIB1 dimerization. It enables light-dependent control of Cre-mediated recombination and has been functionally characterized in mammalian cells and rodent brain.

Need inducible protein relocalization or recruitment

Derived

PA-Cre2.0 is a photoactivatable split Cre recombinase gene switch in which Cre activity is reconstituted by light-induced CRY2-CIB1 dimerization. It enables light-dependent control of Cre-mediated recombination and has been functionally characterized in mammalian cells and rodent brain.

Need precise spatiotemporal control with light input

Derived

PA-Cre2.0 is a photoactivatable split Cre recombinase gene switch in which Cre activity is reconstituted by light-induced CRY2-CIB1 dimerization. It enables light-dependent control of Cre-mediated recombination and has been functionally characterized in mammalian cells and rodent brain.

Taxonomy & Function

Primary hierarchy

Mechanism Branch

Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.

Techniques

No technique tags yet.

Target processes

localizationrecombination

Input: Light

Implementation Constraints

cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: actuatoroperating role: regulatorswitch architecture: multi componentswitch architecture: recruitmentswitch architecture: split

PA-Cre2.0 is implemented as a split Cre recombinase whose fragments are reconstituted through fusion to the light-responsive CRY2-CIB1 dimerization pair. The evidence supports use in mammalian cells and rodent brain, but the supplied material does not specify construct architecture, promoters, delivery method, or exact light wavelength.

The supplied evidence does not provide quantitative performance metrics, illumination parameters, or direct comparisons to alternative photoactivatable Cre systems. Independent replication is not documented in the provided material, and validation is only explicitly stated for mammalian cells and rodent brain.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Supporting Sources

Ranked Claims

Claim 1general conclusionsupports2019Source 1needs review

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
Claim 2general conclusionsupports2019Source 1needs review

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
Claim 3general conclusionsupports2019Source 1needs review

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
Claim 4general conclusionsupports2019Source 1needs review

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
Claim 5general conclusionsupports2019Source 1needs review

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
Claim 6general conclusionsupports2019Source 1needs review

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
Claim 7general conclusionsupports2019Source 1needs review

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
Claim 8general conclusionsupports2019Source 1needs review

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
Claim 9general conclusionsupports2019Source 1needs review

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
Claim 10general conclusionsupports2019Source 1needs review

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
Claim 11general conclusionsupports2019Source 1needs review

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
Claim 12general conclusionsupports2019Source 1needs review

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
Claim 13general conclusionsupports2019Source 1needs review

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
Claim 14general conclusionsupports2019Source 1needs review

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
Claim 15general conclusionsupports2019Source 1needs review

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
Claim 16general conclusionsupports2019Source 1needs review

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
Claim 17general conclusionsupports2019Source 1needs review

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.
Section: abstract
Claim 18in vivo performancesupports2019Source 1needs review

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
Claim 19in vivo performancesupports2019Source 1needs review

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
Claim 20in vivo performancesupports2019Source 1needs review

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
Claim 21in vivo performancesupports2019Source 1needs review

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
Claim 22in vivo performancesupports2019Source 1needs review

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
Claim 23in vivo performancesupports2019Source 1needs review

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
Claim 24in vivo performancesupports2019Source 1needs review

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
Claim 25in vivo performancesupports2019Source 1needs review

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
Claim 26in vivo performancesupports2019Source 1needs review

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
Claim 27in vivo performancesupports2019Source 1needs review

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
Claim 28in vivo performancesupports2019Source 1needs review

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
Claim 29in vivo performancesupports2019Source 1needs review

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
Claim 30in vivo performancesupports2019Source 1needs review

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
Claim 31in vivo performancesupports2019Source 1needs review

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
Claim 32in vivo performancesupports2019Source 1needs review

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
Claim 33in vivo performancesupports2019Source 1needs review

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
Claim 34in vivo performancesupports2019Source 1needs review

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs
Section: abstract
Claim 35mechanismsupports2019Source 1needs review

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
Claim 36mechanismsupports2019Source 1needs review

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
Claim 37mechanismsupports2019Source 1needs review

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
Claim 38mechanismsupports2019Source 1needs review

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
Claim 39mechanismsupports2019Source 1needs review

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
Claim 40mechanismsupports2019Source 1needs review

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
Claim 41mechanismsupports2019Source 1needs review

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
Claim 42mechanismsupports2019Source 1needs review

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
Claim 43mechanismsupports2019Source 1needs review

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
Claim 44mechanismsupports2019Source 1needs review

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
Claim 45mechanismsupports2019Source 1needs review

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
Claim 46mechanismsupports2019Source 1needs review

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
Claim 47mechanismsupports2019Source 1needs review

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
Claim 48mechanismsupports2019Source 1needs review

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
Claim 49mechanismsupports2019Source 1needs review

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
Claim 50mechanismsupports2019Source 1needs review

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
Claim 51mechanismsupports2019Source 1needs review

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization
Section: abstract
Claim 52mechanistic explanationsupports2019Source 1needs review

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
Claim 53mechanistic explanationsupports2019Source 1needs review

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
Claim 54mechanistic explanationsupports2019Source 1needs review

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
Claim 55mechanistic explanationsupports2019Source 1needs review

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
Claim 56mechanistic explanationsupports2019Source 1needs review

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
Claim 57mechanistic explanationsupports2019Source 1needs review

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
Claim 58mechanistic explanationsupports2019Source 1needs review

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
Claim 59mechanistic explanationsupports2019Source 1needs review

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
Claim 60mechanistic explanationsupports2019Source 1needs review

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
Claim 61mechanistic explanationsupports2019Source 1needs review

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
Claim 62mechanistic explanationsupports2019Source 1needs review

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
Claim 63mechanistic explanationsupports2019Source 1needs review

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
Claim 64mechanistic explanationsupports2019Source 1needs review

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
Claim 65mechanistic explanationsupports2019Source 1needs review

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
Claim 66mechanistic explanationsupports2019Source 1needs review

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
Claim 67mechanistic explanationsupports2019Source 1needs review

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
Claim 68mechanistic explanationsupports2019Source 1needs review

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.
Section: abstract
Claim 69performancesupports2019Source 1needs review

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
Claim 70performancesupports2019Source 1needs review

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
Claim 71performancesupports2019Source 1needs review

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
Claim 72performancesupports2019Source 1needs review

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
Claim 73performancesupports2019Source 1needs review

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
Claim 74performancesupports2019Source 1needs review

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
Claim 75performancesupports2019Source 1needs review

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
Claim 76performancesupports2019Source 1needs review

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
Claim 77performancesupports2019Source 1needs review

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
Claim 78performancesupports2019Source 1needs review

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
Claim 79performancesupports2019Source 1needs review

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
Claim 80performancesupports2019Source 1needs review

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
Claim 81performancesupports2019Source 1needs review

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
Claim 82performancesupports2019Source 1needs review

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
Claim 83performancesupports2019Source 1needs review

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
Claim 84performancesupports2019Source 1needs review

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract
Claim 85performancesupports2019Source 1needs review

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels
Section: abstract

Approval Evidence

1 source5 linked approval claimsfirst-pass slug pa-cre2-0
We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization

Source:

general conclusionsupports

This work demonstrates in vivo functionality of PA-Cre2.0 and provides general guidelines for engineering and application of split protein fragments.

This work demonstrates in vivo functionality of PA-Cre2.0, describes new approaches to achieve tight inducible control of Cre DNA recombinase, and provides general guidelines for further engineering and application of split protein fragments.

Source:

in vivo performancesupports

In vivo in rodent brain, PA-Cre2.0 shows low background and sensitive response to brief light inputs.

while in vivo the system also shows low background and sensitive response to brief light inputs

Source:

mechanismsupports

PA-Cre2.0 is a split Cre recombinase reconstituted by light-induced CRY2-CIB1 dimerization.

We characterize a previously developed split Cre recombinase (PA-Cre2.0) that is reconstituted upon light-induced CRY2-CIB1 dimerization

Source:

mechanistic explanationsupports

The consistent activity of PA-Cre2.0 stems from fragment compartmentalization that shifts localization toward the cytosol.

The consistent activity stems from fragment compartmentalization that shifts localization toward the cytosol.

Source:

performancesupports

In cultured cells, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels.

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels

Source:

Comparisons

Source-backed strengths

The literature reports in vivo functionality of PA-Cre2.0 in rodent brain. It is described as having low background activity and a sensitive response to brief light inputs, indicating favorable switching behavior for optical induction of recombination.

Source:

In culture, PA-Cre2.0 shows low background and high induced activity over a wide range of expression levels

Compared with Cry2/CIB

PA-Cre2.0 and Cry2/CIB address a similar problem space because they share localization, recombination.

Shared frame: same top-level item type; shared target processes: localization, recombination; shared mechanisms: heterodimerization; same primary input modality: light

Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.

Compared with CRY2-talin/CIBN-CAAX optogenetic plasma membrane recruitment system

PA-Cre2.0 and CRY2-talin/CIBN-CAAX optogenetic plasma membrane recruitment system address a similar problem space because they share localization, recombination.

Shared frame: same top-level item type; shared target processes: localization, recombination; shared mechanisms: heterodimerization, light-induced heterodimerization; same primary input modality: light

Compared with iLID/SspB

PA-Cre2.0 and iLID/SspB address a similar problem space because they share localization, recombination.

Shared frame: same top-level item type; shared target processes: localization, recombination; shared mechanisms: heterodimerization; same primary input modality: light

Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.

Ranked Citations

  1. 1.
    StructuralSource 1Nucleic Acids Research2019Claim 11Claim 12Claim 11

    Extracted from this source document.