Source 1primary paper2018Cell Reports
Toolkit/PA-Tet-OFF/ON system
PA-Tet-OFF/ON system
Also known as: photoactivatable Tet-OFF/ON system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The PA-Tet-OFF/ON system is a photoactivatable version of the Tet gene expression platform that incorporates the Cry2-CIB1 light-inducible binding switch to control expression of a gene of interest. It enables light- and drug-regulated transcription with precise temporal control and was demonstrated for optogenetic regulation of exogenous gene expression in developing and adult mouse brains.
Usefulness & Problems
Why this is useful
This system is useful for imposing precise temporal control over transgene expression with single-cell resolution in mammalian contexts. The reported in vivo use in developing and adult mouse brains indicates utility for optogenetic manipulation of exogenous gene expression in neural tissue.
Source:
We also demonstrate the optogenetic regulation of exogenous gene expression in vivo, such as in developing and adult mouse brains.
Source:
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Problem solved
It addresses the problem of controlling Tet-based gene expression with light rather than relying only on conventional chemical regulation. The system specifically provides a way to regulate a gene of interest through combined light illumination and drug application.
Source:
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The design integrates the Cry2-CIB1 light-inducible binding switch into the Tet-OFF/ON expression platform, indicating a multi-component construct architecture. The available evidence further indicates that operation requires light illumination and drug application, but the specific construct configuration and experimental delivery details are not provided here.
The supplied evidence does not report quantitative performance metrics such as dynamic range, leakiness, response kinetics, or reversibility. It also does not specify the exact illumination parameters, drug identity, or breadth of validation outside the reported mammalian and mouse brain applications.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The authors demonstrated optogenetic regulation of exogenous gene expression in vivo in developing and adult mouse brains.
We also demonstrate the optogenetic regulation of exogenous gene expression in vivo, such as in developing and adult mouse brains.
The authors demonstrated optogenetic regulation of exogenous gene expression in vivo in developing and adult mouse brains.
We also demonstrate the optogenetic regulation of exogenous gene expression in vivo, such as in developing and adult mouse brains.
The authors demonstrated optogenetic regulation of exogenous gene expression in vivo in developing and adult mouse brains.
We also demonstrate the optogenetic regulation of exogenous gene expression in vivo, such as in developing and adult mouse brains.
The authors demonstrated optogenetic regulation of exogenous gene expression in vivo in developing and adult mouse brains.
We also demonstrate the optogenetic regulation of exogenous gene expression in vivo, such as in developing and adult mouse brains.
The authors demonstrated optogenetic regulation of exogenous gene expression in vivo in developing and adult mouse brains.
We also demonstrate the optogenetic regulation of exogenous gene expression in vivo, such as in developing and adult mouse brains.
The authors demonstrated optogenetic regulation of exogenous gene expression in vivo in developing and adult mouse brains.
We also demonstrate the optogenetic regulation of exogenous gene expression in vivo, such as in developing and adult mouse brains.
The authors demonstrated optogenetic regulation of exogenous gene expression in vivo in developing and adult mouse brains.
We also demonstrate the optogenetic regulation of exogenous gene expression in vivo, such as in developing and adult mouse brains.
The authors demonstrated optogenetic regulation of exogenous gene expression in vivo in developing and adult mouse brains.
We also demonstrate the optogenetic regulation of exogenous gene expression in vivo, such as in developing and adult mouse brains.
The authors demonstrated optogenetic regulation of exogenous gene expression in vivo in developing and adult mouse brains.
We also demonstrate the optogenetic regulation of exogenous gene expression in vivo, such as in developing and adult mouse brains.
The authors demonstrated optogenetic regulation of exogenous gene expression in vivo in developing and adult mouse brains.
We also demonstrate the optogenetic regulation of exogenous gene expression in vivo, such as in developing and adult mouse brains.
The authors demonstrated optogenetic regulation of exogenous gene expression in vivo in developing and adult mouse brains.
We also demonstrate the optogenetic regulation of exogenous gene expression in vivo, such as in developing and adult mouse brains.
The authors demonstrated optogenetic regulation of exogenous gene expression in vivo in developing and adult mouse brains.
We also demonstrate the optogenetic regulation of exogenous gene expression in vivo, such as in developing and adult mouse brains.
The authors demonstrated optogenetic regulation of exogenous gene expression in vivo in developing and adult mouse brains.
We also demonstrate the optogenetic regulation of exogenous gene expression in vivo, such as in developing and adult mouse brains.
The authors demonstrated optogenetic regulation of exogenous gene expression in vivo in developing and adult mouse brains.
We also demonstrate the optogenetic regulation of exogenous gene expression in vivo, such as in developing and adult mouse brains.
The PA-Tet-OFF/ON system integrates the Cry2-CIB1 light-inducible binding switch to regulate expression of a gene of interest using light illumination and drug application.
By integrating the cryptochrome 2-cryptochrome-interacting basic helix-loop-helix 1 (Cry2-CIB1) light-inducible binding switch, expression of the gene of interest is tightly regulated under the control of light illumination and drug application in our PA-Tet-OFF/ON system.
The PA-Tet-OFF/ON system integrates the Cry2-CIB1 light-inducible binding switch to regulate expression of a gene of interest using light illumination and drug application.
By integrating the cryptochrome 2-cryptochrome-interacting basic helix-loop-helix 1 (Cry2-CIB1) light-inducible binding switch, expression of the gene of interest is tightly regulated under the control of light illumination and drug application in our PA-Tet-OFF/ON system.
The PA-Tet-OFF/ON system integrates the Cry2-CIB1 light-inducible binding switch to regulate expression of a gene of interest using light illumination and drug application.
By integrating the cryptochrome 2-cryptochrome-interacting basic helix-loop-helix 1 (Cry2-CIB1) light-inducible binding switch, expression of the gene of interest is tightly regulated under the control of light illumination and drug application in our PA-Tet-OFF/ON system.
The PA-Tet-OFF/ON system integrates the Cry2-CIB1 light-inducible binding switch to regulate expression of a gene of interest using light illumination and drug application.
By integrating the cryptochrome 2-cryptochrome-interacting basic helix-loop-helix 1 (Cry2-CIB1) light-inducible binding switch, expression of the gene of interest is tightly regulated under the control of light illumination and drug application in our PA-Tet-OFF/ON system.
The PA-Tet-OFF/ON system integrates the Cry2-CIB1 light-inducible binding switch to regulate expression of a gene of interest using light illumination and drug application.
By integrating the cryptochrome 2-cryptochrome-interacting basic helix-loop-helix 1 (Cry2-CIB1) light-inducible binding switch, expression of the gene of interest is tightly regulated under the control of light illumination and drug application in our PA-Tet-OFF/ON system.
The PA-Tet-OFF/ON system integrates the Cry2-CIB1 light-inducible binding switch to regulate expression of a gene of interest using light illumination and drug application.
By integrating the cryptochrome 2-cryptochrome-interacting basic helix-loop-helix 1 (Cry2-CIB1) light-inducible binding switch, expression of the gene of interest is tightly regulated under the control of light illumination and drug application in our PA-Tet-OFF/ON system.
The PA-Tet-OFF/ON system integrates the Cry2-CIB1 light-inducible binding switch to regulate expression of a gene of interest using light illumination and drug application.
By integrating the cryptochrome 2-cryptochrome-interacting basic helix-loop-helix 1 (Cry2-CIB1) light-inducible binding switch, expression of the gene of interest is tightly regulated under the control of light illumination and drug application in our PA-Tet-OFF/ON system.
The PA-Tet-OFF/ON system integrates the Cry2-CIB1 light-inducible binding switch to regulate expression of a gene of interest using light illumination and drug application.
By integrating the cryptochrome 2-cryptochrome-interacting basic helix-loop-helix 1 (Cry2-CIB1) light-inducible binding switch, expression of the gene of interest is tightly regulated under the control of light illumination and drug application in our PA-Tet-OFF/ON system.
The PA-Tet-OFF/ON system integrates the Cry2-CIB1 light-inducible binding switch to regulate expression of a gene of interest using light illumination and drug application.
By integrating the cryptochrome 2-cryptochrome-interacting basic helix-loop-helix 1 (Cry2-CIB1) light-inducible binding switch, expression of the gene of interest is tightly regulated under the control of light illumination and drug application in our PA-Tet-OFF/ON system.
The PA-Tet-OFF/ON system integrates the Cry2-CIB1 light-inducible binding switch to regulate expression of a gene of interest using light illumination and drug application.
By integrating the cryptochrome 2-cryptochrome-interacting basic helix-loop-helix 1 (Cry2-CIB1) light-inducible binding switch, expression of the gene of interest is tightly regulated under the control of light illumination and drug application in our PA-Tet-OFF/ON system.
The PA-Tet-OFF/ON system integrates the Cry2-CIB1 light-inducible binding switch to regulate expression of a gene of interest using light illumination and drug application.
By integrating the cryptochrome 2-cryptochrome-interacting basic helix-loop-helix 1 (Cry2-CIB1) light-inducible binding switch, expression of the gene of interest is tightly regulated under the control of light illumination and drug application in our PA-Tet-OFF/ON system.
The PA-Tet-OFF/ON system integrates the Cry2-CIB1 light-inducible binding switch to regulate expression of a gene of interest using light illumination and drug application.
By integrating the cryptochrome 2-cryptochrome-interacting basic helix-loop-helix 1 (Cry2-CIB1) light-inducible binding switch, expression of the gene of interest is tightly regulated under the control of light illumination and drug application in our PA-Tet-OFF/ON system.
The PA-Tet-OFF/ON system integrates the Cry2-CIB1 light-inducible binding switch to regulate expression of a gene of interest using light illumination and drug application.
By integrating the cryptochrome 2-cryptochrome-interacting basic helix-loop-helix 1 (Cry2-CIB1) light-inducible binding switch, expression of the gene of interest is tightly regulated under the control of light illumination and drug application in our PA-Tet-OFF/ON system.
The PA-Tet-OFF/ON system integrates the Cry2-CIB1 light-inducible binding switch to regulate expression of a gene of interest using light illumination and drug application.
By integrating the cryptochrome 2-cryptochrome-interacting basic helix-loop-helix 1 (Cry2-CIB1) light-inducible binding switch, expression of the gene of interest is tightly regulated under the control of light illumination and drug application in our PA-Tet-OFF/ON system.
The PA-Tet-OFF/ON system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
This system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
The PA-Tet-OFF/ON system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
This system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
The PA-Tet-OFF/ON system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
This system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
The PA-Tet-OFF/ON system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
This system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
The PA-Tet-OFF/ON system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
This system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
The PA-Tet-OFF/ON system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
This system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
The PA-Tet-OFF/ON system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
This system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
The PA-Tet-OFF/ON system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
This system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
The PA-Tet-OFF/ON system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
This system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
The PA-Tet-OFF/ON system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
This system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
The PA-Tet-OFF/ON system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
This system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
The PA-Tet-OFF/ON system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
This system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
The PA-Tet-OFF/ON system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
This system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
The PA-Tet-OFF/ON system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
This system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
The authors developed a photoactivatable PA-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
The authors developed a photoactivatable PA-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
The authors developed a photoactivatable PA-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
The authors developed a photoactivatable PA-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
The authors developed a photoactivatable PA-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
The authors developed a photoactivatable PA-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
The authors developed a photoactivatable PA-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
The authors developed a photoactivatable PA-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
The authors developed a photoactivatable PA-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
The authors developed a photoactivatable PA-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
The authors developed a photoactivatable PA-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
The authors developed a photoactivatable PA-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
The authors developed a photoactivatable PA-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
The authors developed a photoactivatable PA-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug pa-tet-off-on-system
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Source:
in vivo applicationsupports
The authors demonstrated optogenetic regulation of exogenous gene expression in vivo in developing and adult mouse brains.
We also demonstrate the optogenetic regulation of exogenous gene expression in vivo, such as in developing and adult mouse brains.
Source:
mechanism or designsupports
The PA-Tet-OFF/ON system integrates the Cry2-CIB1 light-inducible binding switch to regulate expression of a gene of interest using light illumination and drug application.
By integrating the cryptochrome 2-cryptochrome-interacting basic helix-loop-helix 1 (Cry2-CIB1) light-inducible binding switch, expression of the gene of interest is tightly regulated under the control of light illumination and drug application in our PA-Tet-OFF/ON system.
Source:
performancesupports
The PA-Tet-OFF/ON system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
This system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
Source:
tool developmentsupports
The authors developed a photoactivatable PA-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Here, we develop a photoactivatable (PA)-Tet-OFF/ON system for precise temporal control of gene expression at single-cell resolution.
Source:
Comparisons
Source-backed strengths
The reported strengths are precise temporal control of gene expression and single-cell resolution. The system was also demonstrated in vivo for optogenetic regulation of exogenous gene expression in both developing and adult mouse brains.
Source:
This system has a large dynamic range of downstream gene expression and rapid activation/deactivation kinetics.
Ranked Citations
- 1.