Source 1review2016Biophysical Journal
Toolkit/paCas9
paCas9
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The supplied web research summary states that the review spans photoactivatable genome editing and that the directly matching primary paper identifies the split Cas9 light-inducible system as paCas9 using pMag and nMag.
Usefulness & Problems
No literature-backed usefulness or problem-fit explainer has been materialized for this record yet.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
editingInput: Light
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
GCaMP6s is used in the review context as an activity reporter example for zebrafish brain imaging.
The anchor figure caption explicitly cites GCaMP6s for zebrafish brain activity imaging.
LOVpep and ePDZ are presented in the review context as a light-induced intracellular trafficking control system.
The anchor figure caption names LOVpep and ePDZ in a light-induced trafficking example.
Optopatch is presented in the review context as an all-optical electrophysiology system with CheRiff and QuasAr2 as named components.
PubMed figure captions ... explicitly mention tool/component names used in the review, especially Optopatch/CheRiff/QuasAr2.
pMag and nMag are presented in the review context as a light-induced dimerization pair used to reconstitute split Cas9 in photoactivatable genome editing.
The anchor figure caption names pMag as one half of the light-induced dimerization pair used to reconstitute split Cas9 ... nMag as the partner to pMag.
Approval Evidence
1 source1 linked approval claimfirst-pass slug pacas9
The supplied web research summary states that the review spans photoactivatable genome editing and that the directly matching primary paper identifies the split Cas9 light-inducible system as paCas9 using pMag and nMag.
Source:
tool mechanismsupports
pMag and nMag are presented in the review context as a light-induced dimerization pair used to reconstitute split Cas9 in photoactivatable genome editing.
The anchor figure caption names pMag as one half of the light-induced dimerization pair used to reconstitute split Cas9 ... nMag as the partner to pMag.
Source:
Comparisons
No literature-backed comparison notes have been materialized for this record yet.
Ranked Citations
- 1.