Source 1primary paper2025MED
Toolkit/PhoBIT2
PhoBIT2
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
PhoBIT2, a light-ON switch building upon an evolved ssrA/CRY2-sspB pair with minimal basal interaction
Usefulness & Problems
No literature-backed usefulness or problem-fit explainer has been materialized for this record yet.
Published Workflows
Objective: Engineer photo-inducible binary interaction tools based on the ssrA-sspB system for optical control of diverse protein functions and biomedical applications.
Why it works: The workflow is motivated by the compact size of the 7-residue ssrA peptide, which the abstract states is ideal for insertion into proteins of interest, enabling host protein manipulation through engineered light-responsive sspB-based systems.
light-OFF switching via LOV2 integration into sspBlight-ON switching via an evolved ssrA/CRY2-sspB pairtargeted ssrA insertion into proteins of interestprotein engineeringoptogenetic tool developmenttargeted peptide-tag insertion
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
signalingInput: Light
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
When incorporated into a monobody, PhoBIT2 enables photo-switchable inhibition of an oncogenic fusion protein and curtails leukemogenesis in vivo.
PhoBIT tools enable mechanistic dissection and optogenetic modulation of GPCRs, ion channels, necroptosis, and innate immune signaling.
PhoBIT1 is a light-OFF photo-inducible binary interaction tool generated by integrating LOV2 into sspB.
PhoBIT2 is a light-ON photo-inducible binary interaction tool built on an evolved ssrA/CRY2-sspB pair with minimal basal interaction.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug phobit2
PhoBIT2, a light-ON switch building upon an evolved ssrA/CRY2-sspB pair with minimal basal interaction
Source:
application resultsupports
When incorporated into a monobody, PhoBIT2 enables photo-switchable inhibition of an oncogenic fusion protein and curtails leukemogenesis in vivo.
Source:
application scopesupports
PhoBIT tools enable mechanistic dissection and optogenetic modulation of GPCRs, ion channels, necroptosis, and innate immune signaling.
Source:
tool descriptionsupports
PhoBIT2 is a light-ON photo-inducible binary interaction tool built on an evolved ssrA/CRY2-sspB pair with minimal basal interaction.
Source:
Comparisons
No literature-backed comparison notes have been materialized for this record yet.
Ranked Citations
- 1.