Source 1primary paper2003Biochemistry
Toolkit/PHOT1a N-terminus upstream of LOV1
PHOT1a N-terminus upstream of LOV1
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The PHOT1a N-terminus upstream of LOV1 is a region of Avena sativa phototropin 1a identified as an autophosphorylation region. Available evidence localizes phosphorylation activity to this segment but does not describe an engineered output or standalone tool function.
Usefulness & Problems
Why this is useful
This region is useful as a mapped phosphorylation-bearing segment within phototropin 1a for studies of receptor regulation and domain-level signaling architecture. The evidence supports its value primarily for mechanistic dissection of phototropin autophosphorylation rather than for direct applied optogenetic use.
Problem solved
It helps solve the problem of localizing where autophosphorylation occurs within Avena sativa PHOT1a. Specifically, the cited work assigns the N-terminus upstream of LOV1 as one of the autophosphorylation regions.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Mechanisms
autophosphorylationTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Implementation Constraints
The evidence identifies this segment as part of Avena sativa phototropin 1a and places it upstream of the LOV1 domain. No practical implementation details such as construct design, expression system, cofactors, illumination conditions, or delivery strategy are provided in the supplied evidence.
Evidence is limited to a single mapping claim from one study and does not provide sequence boundaries, residue-level phosphosite identification, or quantitative phosphorylation properties in the supplied material. No engineered construct performance, transferability, or independent replication is described here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
In Avena sativa PHOT1a, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2 are autophosphorylation regions.
We describe here two additional domains, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2, as the regions for autophosphorylation
In Avena sativa PHOT1a, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2 are autophosphorylation regions.
We describe here two additional domains, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2, as the regions for autophosphorylation
In Avena sativa PHOT1a, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2 are autophosphorylation regions.
We describe here two additional domains, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2, as the regions for autophosphorylation
In Avena sativa PHOT1a, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2 are autophosphorylation regions.
We describe here two additional domains, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2, as the regions for autophosphorylation
In Avena sativa PHOT1a, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2 are autophosphorylation regions.
We describe here two additional domains, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2, as the regions for autophosphorylation
In Avena sativa PHOT1a, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2 are autophosphorylation regions.
We describe here two additional domains, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2, as the regions for autophosphorylation
In Avena sativa PHOT1a, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2 are autophosphorylation regions.
We describe here two additional domains, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2, as the regions for autophosphorylation
Approval Evidence
1 source1 linked approval claimfirst-pass slug phot1a-n-terminus-upstream-of-lov1
the N-terminus upstream of LOV1 ... as the regions for autophosphorylation
Source:
domain localizationsupports
In Avena sativa PHOT1a, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2 are autophosphorylation regions.
We describe here two additional domains, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2, as the regions for autophosphorylation
Source:
Comparisons
Source-backed strengths
The main strength is direct domain-level localization of autophosphorylation within a defined portion of PHOT1a. The evidence also places this region in the context of another autophosphorylation region, the hinge between LOV1 and LOV2, supporting a mapped intramolecular phosphorylation landscape.
Ranked Citations
- 1.