Source 1primary paper2022
Toolkit/photo-sensitive circular gRNAs
photo-sensitive circular gRNAs
Also known as: circular gRNA, cyclically caged guide RNAs
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Photo-sensitive circular gRNAs are cyclically caged guide RNAs that enable light-activated CRISPR/Cas9- and Cpf1-mediated genome editing. They are designed for spatiotemporal control of editing and are activated by photocleavage of the circularized guide.
Usefulness & Problems
Why this is useful
This tool is useful for controlling when and where CRISPR editing occurs using light. The cited work presents it as an improved method for precise spatiotemporal manipulation of gene editing and reports efficient editing with CRISPR/Cas9 and Cpf1.
Source:
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
Source:
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Problem solved
It addresses the problem of limited temporal and spatial precision in genome editing. The reported design also reduces unwanted activity before activation, as a bow-knot-type gRNA showed no background editing without light irradiation.
Source:
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
Problem links
Need controllable genome or transcript editing
DerivedPhoto-sensitive circular gRNAs are cyclically caged guide RNAs designed to enable light-controlled CRISPR/Cas9- and Cpf1-mediated genome editing. They support spatiotemporal regulation of editing and can be activated by photocleavage of the circularized guide.
Need precise spatiotemporal control with light input
DerivedPhoto-sensitive circular gRNAs are cyclically caged guide RNAs designed to enable light-controlled CRISPR/Cas9- and Cpf1-mediated genome editing. They support spatiotemporal regulation of editing and can be activated by photocleavage of the circularized guide.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level RNA part used inside a larger architecture that realizes a mechanism.
Techniques
No technique tags yet.
Target processes
editingInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: cleavage
Implementation involves circularized, photo-sensitive guide RNAs used with CRISPR/Cas9 or Cpf1 systems. The evidence supports covalent cyclization and chemical modification conceptually, but it does not provide construct architecture, delivery method, or cofactor requirements.
The supplied evidence does not specify the photocleavable chemistry, illumination wavelength, activation kinetics, or quantitative editing efficiencies. Validation is currently supported by a single cited study, with embryo editing mentioned only for MSTN.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug photo-sensitive-circular-grnas
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Source:
application scopesupports
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
Source:
embryo editingsupports
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Source:
engineering method advantagesupports
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
Source:
functional capabilitysupports
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Source:
light gated editingsupports
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
Source:
Comparisons
Source-backed strengths
Reported strengths include spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing. The source also states that a bow-knot-type gRNA had no detectable background editing in the absence of light and that light-mediated MSTN gene editing was achieved in embryos.
Source:
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
photo-sensitive circular gRNAs and auxiliary photocleavable oligodeoxyribonucleotides complementary to crRNA address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; shared mechanisms: photocleavage; same primary input modality: light
Compared with caged guide RNA
photo-sensitive circular gRNAs and caged guide RNA address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; shared mechanisms: photocleavage; same primary input modality: light
Compared with light-controlled crRNA
photo-sensitive circular gRNAs and light-controlled crRNA address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; shared mechanisms: photocleavage; same primary input modality: light
Ranked Citations
- 1.