Source 1primary paper2016Nature Chemical Biology
Toolkit/photoactivatable Cre recombinase
photoactivatable Cre recombinase
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Photoactivatable Cre recombinase is a light-controlled recombination tool reported in a 2016 Nature Chemical Biology study. The available evidence links it to optimized second-generation CRY2–CIB dimerizers and indicates that it enables light regulation of Cre-mediated recombination.
Usefulness & Problems
Why this is useful
This tool is useful for coupling Cre recombinase activity to light input rather than constitutive or chemically induced control. The supplied evidence supports its use for optical control of recombination, but does not provide quantitative performance or application context.
Source:
The paper reports a photoactivatable Cre recombinase.
Problem solved
It addresses the problem of making Cre-mediated recombination photoresponsive. The evidence indicates that the study reported a photoactivatable Cre recombinase, but does not specify the exact experimental limitations it was designed to overcome.
Problem links
Need conditional recombination or state switching
DerivedPhotoactivatable Cre recombinase is a light-controlled recombination tool reported in a 2016 Nature Chemical Biology study titled "Optimized second-generation CRY2–CIB dimerizers and photoactivatable Cre recombinase." The available evidence indicates that it is associated with optimized CRY2–CIB dimerizers and is used to control Cre-mediated recombination with light.
Need precise spatiotemporal control with light input
DerivedPhotoactivatable Cre recombinase is a light-controlled recombination tool reported in a 2016 Nature Chemical Biology study titled "Optimized second-generation CRY2–CIB dimerizers and photoactivatable Cre recombinase." The available evidence indicates that it is associated with optimized CRY2–CIB dimerizers and is used to control Cre-mediated recombination with light.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
recombinationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: actuatoroperating role: regulatorswitch architecture: multi componentswitch architecture: recruitment
The available evidence associates the tool with CRY2–CIB dimerizers, implying a multi-component optogenetic design responsive to light. However, the supplied material does not specify construct architecture, expression system, chromophore requirements, illumination parameters, or delivery method.
The evidence is limited to the study title and high-level claims, so details on dynamic range, background recombination, wavelength dependence, kinetics, and biological validation are not available here. Independent replication is also not established by the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports optimized second-generation CRY2–CIB dimerizers.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
The paper reports a photoactivatable Cre recombinase.
Approval Evidence
1 source1 linked approval claimfirst-pass slug photoactivatable-cre-recombinase
Optimized second-generation CRY2–CIB dimerizers and photoactivatable Cre recombinase
Source:
tool reportsupports
The paper reports a photoactivatable Cre recombinase.
Source:
Comparisons
Source-backed strengths
A key strength supported by the evidence is that the tool provides light-controlled Cre recombinase function. The same study also reports optimized second-generation CRY2–CIB dimerizers, suggesting an engineered optogenetic context, but no specific strength metrics are provided in the supplied material.
Source:
The paper reports optimized second-generation CRY2–CIB dimerizers.
Compared with AQTrip EL222 variant
photoactivatable Cre recombinase and AQTrip EL222 variant address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; shared mechanisms: heterodimerization; same primary input modality: light
photoactivatable Cre recombinase and CRY2-talin/CIBN-CAAX optogenetic plasma membrane recruitment system address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; shared mechanisms: heterodimerization; same primary input modality: light
Compared with PA-Cre 3.0
photoactivatable Cre recombinase and PA-Cre 3.0 address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; shared mechanisms: heterodimerization; same primary input modality: light
Ranked Citations
- 1.