Source 1primary paper2024Analytical Chemistry
Toolkit/photoactivatable CRISPR/Cas12a system
photoactivatable CRISPR/Cas12a system
Also known as: photoactivatable CRISPR/Cas12a platform, photoactivatable CRISPR/Cas12a sensors, photoactivatable CRISPR–Cas12 system, photoactivated CRISPR-Cas12a, photoactivation CRISPR/Cas12a system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The photoactivatable CRISPR/Cas12a system is a light-gated nucleic acid sensing platform that integrates photoactivation with CRISPR/Cas12a for DNA and RNA detection. It has been used in visual assay formats, including HPV16 detection and biomarker imaging, to provide spatiotemporal control over Cas12a-based sensing.
Usefulness & Problems
Why this is useful
This system is useful because it adds light-dependent temporal and spatial control to CRISPR/Cas12a nucleic acid detection. Reported applications include visual readouts, lateral flow strip-based signal visualization, and potential point-of-care or on-site diagnostic formats.
Source:
to enable simple, rapid and convenient visualization detection of HPV16, facilitated by blue UV light at 302 nm
Source:
Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for survivin by photoactivation in vivo, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
Problem solved
It addresses the problem of controlling when and where Cas12a-based nucleic acid sensing is activated during diagnostic assays. The reported implementations also target practical visual detection of analytes such as HPV16 by combining photoactivation with amplification and portable readout formats.
Source:
to enable simple, rapid and convenient visualization detection of HPV16, facilitated by blue UV light at 302 nm
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
PhotocleavageTarget processes
diagnosticeditingrecombinationInput: Light
Implementation Constraints
Reported implementations integrate photoactivation with CRISPR/Cas12a and have been used for both DNA and RNA detection. One HPV16 assay combined photoactivated CRISPR-Cas12a with a tube-in-tube structure and recombinase polymerase amplification, and another report used lateral flow assay strips to visualize nucleic acid cleavage signals; activation was facilitated by blue UV light at 302 nm.
The supplied evidence is limited to application-focused reports and does not provide quantitative performance metrics such as sensitivity, specificity, dynamic range, or activation kinetics. The need for light activation at 302 nm and the use of multi-component assay formats may impose practical constraints, but the evidence does not detail their impact systematically.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successMouseapplication demo
fluorescent sensing
Inferred from claim c4 during normalization. The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage. Derived from claim c4. Quoted text: Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for <i>survivin</i> by photoactivation <i>in vivo</i>, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
Source:
successMouseapplication demo
fluorescent sensing
Inferred from claim c4 during normalization. The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage. Derived from claim c4. Quoted text: Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for <i>survivin</i> by photoactivation <i>in vivo</i>, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
Source:
successMouseapplication demo
fluorescent sensing
Inferred from claim c4 during normalization. The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage. Derived from claim c4. Quoted text: Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for <i>survivin</i> by photoactivation <i>in vivo</i>, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
Source:
successMouseapplication demo
fluorescent sensing
Inferred from claim c4 during normalization. The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage. Derived from claim c4. Quoted text: Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for <i>survivin</i> by photoactivation <i>in vivo</i>, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
Source:
successMouseapplication demo
fluorescent sensing
Inferred from claim c4 during normalization. The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage. Derived from claim c4. Quoted text: Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for <i>survivin</i> by photoactivation <i>in vivo</i>, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
Source:
successMouseapplication demo
fluorescent sensing
Inferred from claim c4 during normalization. The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage. Derived from claim c4. Quoted text: Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for <i>survivin</i> by photoactivation <i>in vivo</i>, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
Source:
successMouseapplication demo
fluorescent sensing
Inferred from claim c4 during normalization. The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage. Derived from claim c4. Quoted text: Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for <i>survivin</i> by photoactivation <i>in vivo</i>, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
Source:
Supporting Sources
Source 2primary paper2025Chemical Communications
Ranked Claims
The combined system enables visual detection of HPV16 facilitated by blue UV light at 302 nm.
to enable simple, rapid and convenient visualization detection of HPV16, facilitated by blue UV light at 302 nm
illumination wavelength 302 nm
The combined system enables visual detection of HPV16 facilitated by blue UV light at 302 nm.
to enable simple, rapid and convenient visualization detection of HPV16, facilitated by blue UV light at 302 nm
illumination wavelength 302 nm
The combined system enables visual detection of HPV16 facilitated by blue UV light at 302 nm.
to enable simple, rapid and convenient visualization detection of HPV16, facilitated by blue UV light at 302 nm
illumination wavelength 302 nm
The combined system enables visual detection of HPV16 facilitated by blue UV light at 302 nm.
to enable simple, rapid and convenient visualization detection of HPV16, facilitated by blue UV light at 302 nm
illumination wavelength 302 nm
The combined system enables visual detection of HPV16 facilitated by blue UV light at 302 nm.
to enable simple, rapid and convenient visualization detection of HPV16, facilitated by blue UV light at 302 nm
illumination wavelength 302 nm
The combined system enables visual detection of HPV16 facilitated by blue UV light at 302 nm.
to enable simple, rapid and convenient visualization detection of HPV16, facilitated by blue UV light at 302 nm
illumination wavelength 302 nm
The study combines photoactivated CRISPR-Cas12a, a tube-in-tube structure, and recombinase polymerase amplification for visual detection of HPV16.
we have combined photoactivated CRISPR-Cas12a with tube-in-tube structure and recombinase polymerase amplification (RPA) to enable simple, rapid and convenient visualization detection of HPV16
The study combines photoactivated CRISPR-Cas12a, a tube-in-tube structure, and recombinase polymerase amplification for visual detection of HPV16.
we have combined photoactivated CRISPR-Cas12a with tube-in-tube structure and recombinase polymerase amplification (RPA) to enable simple, rapid and convenient visualization detection of HPV16
The study combines photoactivated CRISPR-Cas12a, a tube-in-tube structure, and recombinase polymerase amplification for visual detection of HPV16.
we have combined photoactivated CRISPR-Cas12a with tube-in-tube structure and recombinase polymerase amplification (RPA) to enable simple, rapid and convenient visualization detection of HPV16
The study combines photoactivated CRISPR-Cas12a, a tube-in-tube structure, and recombinase polymerase amplification for visual detection of HPV16.
we have combined photoactivated CRISPR-Cas12a with tube-in-tube structure and recombinase polymerase amplification (RPA) to enable simple, rapid and convenient visualization detection of HPV16
The study combines photoactivated CRISPR-Cas12a, a tube-in-tube structure, and recombinase polymerase amplification for visual detection of HPV16.
we have combined photoactivated CRISPR-Cas12a with tube-in-tube structure and recombinase polymerase amplification (RPA) to enable simple, rapid and convenient visualization detection of HPV16
The study combines photoactivated CRISPR-Cas12a, a tube-in-tube structure, and recombinase polymerase amplification for visual detection of HPV16.
we have combined photoactivated CRISPR-Cas12a with tube-in-tube structure and recombinase polymerase amplification (RPA) to enable simple, rapid and convenient visualization detection of HPV16
The system is presented as a potential tool for on-site diagnostic use with possible portability and speed benefits.
It serves as a potential tool for on-site diagnostic use, which could be beneficial in terms of portability and speed.
The system is presented as a potential tool for on-site diagnostic use with possible portability and speed benefits.
It serves as a potential tool for on-site diagnostic use, which could be beneficial in terms of portability and speed.
The system is presented as a potential tool for on-site diagnostic use with possible portability and speed benefits.
It serves as a potential tool for on-site diagnostic use, which could be beneficial in terms of portability and speed.
The system is presented as a potential tool for on-site diagnostic use with possible portability and speed benefits.
It serves as a potential tool for on-site diagnostic use, which could be beneficial in terms of portability and speed.
The system is presented as a potential tool for on-site diagnostic use with possible portability and speed benefits.
It serves as a potential tool for on-site diagnostic use, which could be beneficial in terms of portability and speed.
The system is presented as a potential tool for on-site diagnostic use with possible portability and speed benefits.
It serves as a potential tool for on-site diagnostic use, which could be beneficial in terms of portability and speed.
Combining a lateral flow assay strip test with the CRISPR/Cas12a system enabled visualization of nucleic acid cleavage signals and suggested instant test application potential.
We combined the lateral flow assay strip test with the CRISPR/Cas12a system to realize the visualization of nucleic acid cleavage signals, displaying potential instant test application capabilities.
Combining a lateral flow assay strip test with the CRISPR/Cas12a system enabled visualization of nucleic acid cleavage signals and suggested instant test application potential.
We combined the lateral flow assay strip test with the CRISPR/Cas12a system to realize the visualization of nucleic acid cleavage signals, displaying potential instant test application capabilities.
Combining a lateral flow assay strip test with the CRISPR/Cas12a system enabled visualization of nucleic acid cleavage signals and suggested instant test application potential.
We combined the lateral flow assay strip test with the CRISPR/Cas12a system to realize the visualization of nucleic acid cleavage signals, displaying potential instant test application capabilities.
Combining a lateral flow assay strip test with the CRISPR/Cas12a system enabled visualization of nucleic acid cleavage signals and suggested instant test application potential.
We combined the lateral flow assay strip test with the CRISPR/Cas12a system to realize the visualization of nucleic acid cleavage signals, displaying potential instant test application capabilities.
Combining a lateral flow assay strip test with the CRISPR/Cas12a system enabled visualization of nucleic acid cleavage signals and suggested instant test application potential.
We combined the lateral flow assay strip test with the CRISPR/Cas12a system to realize the visualization of nucleic acid cleavage signals, displaying potential instant test application capabilities.
Combining a lateral flow assay strip test with the CRISPR/Cas12a system enabled visualization of nucleic acid cleavage signals and suggested instant test application potential.
We combined the lateral flow assay strip test with the CRISPR/Cas12a system to realize the visualization of nucleic acid cleavage signals, displaying potential instant test application capabilities.
Combining a lateral flow assay strip test with the CRISPR/Cas12a system enabled visualization of nucleic acid cleavage signals and suggested instant test application potential.
We combined the lateral flow assay strip test with the CRISPR/Cas12a system to realize the visualization of nucleic acid cleavage signals, displaying potential instant test application capabilities.
The study integrated photoactivation with CRISPR/Cas12a for DNA and RNA detection to provide high spatiotemporal control of nucleic acid sensing.
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
The study integrated photoactivation with CRISPR/Cas12a for DNA and RNA detection to provide high spatiotemporal control of nucleic acid sensing.
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
The study integrated photoactivation with CRISPR/Cas12a for DNA and RNA detection to provide high spatiotemporal control of nucleic acid sensing.
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
The study integrated photoactivation with CRISPR/Cas12a for DNA and RNA detection to provide high spatiotemporal control of nucleic acid sensing.
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
The study integrated photoactivation with CRISPR/Cas12a for DNA and RNA detection to provide high spatiotemporal control of nucleic acid sensing.
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
The study integrated photoactivation with CRISPR/Cas12a for DNA and RNA detection to provide high spatiotemporal control of nucleic acid sensing.
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
The study integrated photoactivation with CRISPR/Cas12a for DNA and RNA detection to provide high spatiotemporal control of nucleic acid sensing.
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage.
Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for survivin by photoactivation in vivo, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage.
Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for survivin by photoactivation in vivo, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage.
Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for survivin by photoactivation in vivo, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage.
Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for survivin by photoactivation in vivo, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage.
Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for survivin by photoactivation in vivo, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage.
Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for survivin by photoactivation in vivo, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage.
Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for survivin by photoactivation in vivo, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
The photoactivatable CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets.
Our strategy suggests that the CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets
The photoactivatable CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets.
Our strategy suggests that the CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets
The photoactivatable CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets.
Our strategy suggests that the CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets
The photoactivatable CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets.
Our strategy suggests that the CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets
The photoactivatable CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets.
Our strategy suggests that the CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets
The photoactivatable CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets.
Our strategy suggests that the CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets
The photoactivatable CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets.
Our strategy suggests that the CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets
The photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin by rational design of the target recognition sequence.
By rationally designing the target recognition sequence, this photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin, respectively.
The photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin by rational design of the target recognition sequence.
By rationally designing the target recognition sequence, this photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin, respectively.
The photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin by rational design of the target recognition sequence.
By rationally designing the target recognition sequence, this photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin, respectively.
The photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin by rational design of the target recognition sequence.
By rationally designing the target recognition sequence, this photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin, respectively.
The photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin by rational design of the target recognition sequence.
By rationally designing the target recognition sequence, this photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin, respectively.
The photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin by rational design of the target recognition sequence.
By rationally designing the target recognition sequence, this photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin, respectively.
The photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin by rational design of the target recognition sequence.
By rationally designing the target recognition sequence, this photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin, respectively.
Approval Evidence
2 sources8 linked approval claimsfirst-pass slug photoactivatable-crispr-cas12a-system
we have combined photoactivated CRISPR-Cas12a with tube-in-tube structure and recombinase polymerase amplification (RPA)
Source:
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
Source:
applicationsupports
The combined system enables visual detection of HPV16 facilitated by blue UV light at 302 nm.
to enable simple, rapid and convenient visualization detection of HPV16, facilitated by blue UV light at 302 nm
Source:
combination methodsupports
The study combines photoactivated CRISPR-Cas12a, a tube-in-tube structure, and recombinase polymerase amplification for visual detection of HPV16.
we have combined photoactivated CRISPR-Cas12a with tube-in-tube structure and recombinase polymerase amplification (RPA) to enable simple, rapid and convenient visualization detection of HPV16
Source:
potential use casesupports
The system is presented as a potential tool for on-site diagnostic use with possible portability and speed benefits.
It serves as a potential tool for on-site diagnostic use, which could be beneficial in terms of portability and speed.
Source:
assay capabilitysupports
Combining a lateral flow assay strip test with the CRISPR/Cas12a system enabled visualization of nucleic acid cleavage signals and suggested instant test application potential.
We combined the lateral flow assay strip test with the CRISPR/Cas12a system to realize the visualization of nucleic acid cleavage signals, displaying potential instant test application capabilities.
Source:
engineering strategysupports
The study integrated photoactivation with CRISPR/Cas12a for DNA and RNA detection to provide high spatiotemporal control of nucleic acid sensing.
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
Source:
in vivo controlsupports
The study reports temporary in vivo photoactivation control of fluorescent sensing activity for survivin, enabling rapid target nucleic acid detection and reducing contamination risk from premature leaks during storage.
Additionally, we also successfully realized the temporary control of its fluorescent sensing activity for survivin by photoactivation in vivo, allowing rapid detection of target nucleic acids and avoiding the risk of contamination from premature leaks during storage.
Source:
platform generalitysupports
The photoactivatable CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets.
Our strategy suggests that the CRISPR/Cas12a platform can be triggered by photoactivation to sense various targets
Source:
target scopesupports
The photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin by rational design of the target recognition sequence.
By rationally designing the target recognition sequence, this photoactivation CRISPR/Cas12a system could recognize HPV16 and survivin, respectively.
Source:
Comparisons
Source-backed strengths
Reported strengths include high spatiotemporal control for DNA and RNA sensing and compatibility with visual detection workflows. The platform has been combined with recombinase polymerase amplification, a tube-in-tube structure, and lateral flow assay strips, and blue UV light at 302 nm was used to enable visual HPV16 detection.
Source:
Here, we integrated photoactivation with the CRISPR/Cas12a system for DNA and RNA detection, aiming to provide high spatiotemporal control for nucleic acid sensing.
Ranked Citations
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