Source 1primary paper2021Russian Journal of Bioorganic Chemistry
Toolkit/photoactivatable CRISPR/Cas9 system
photoactivatable CRISPR/Cas9 system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The photoactivatable CRISPR/Cas9 system is a light-gated genome-editing configuration comprising Cas9, either a synthetic 102-nt sgRNA or a crRNA/tracrRNA pair, and blocking photocleavable oligodeoxyribonucleotides. UV irradiation in the presence of the photomodified blocking oligodeoxyribonucleotides enables photoactivatable gene editing in vitro.
Usefulness & Problems
Why this is useful
This system is useful for imposing optical control over CRISPR/Cas9-mediated editing by keeping the guide RNA functionally blocked until UV exposure. The available evidence supports in vitro light-triggered activation, but does not further quantify performance or application scope.
Source:
It has been shown that UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can result in photoactivatable gene editing in vitro.
Problem solved
It addresses the problem of making CRISPR/Cas9 editing conditional on an external light input rather than constitutively active once Cas9 and guide RNA are present. The reported design uses blocking photocleavable oligodeoxyribonucleotides to gate editing until UV irradiation.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
PhotocleavageTechniques
Computational DesignTarget processes
editingInput: Light
Implementation Constraints
The reported components are Cas9 protein, either a synthetic 102-nt sgRNA or a guide crRNA/tracrRNA pair, and blocking photocleavable oligodeoxyribonucleotides. Activation requires UV irradiation, but the supplied evidence does not specify wavelength, dose, delivery method, or construct stoichiometry.
The evidence provided is limited to design description and an in vitro functional claim under UV irradiation. No quantitative editing efficiencies, target loci, cell-based validation, organismal use, off-target analysis, or independent replication are described in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
A photoactivatable CRISPR/Cas9 system composed of Cas9, guide RNA, and blocking photocleavable oligodeoxyribonucleotides was designed.
A photoactivatable CRISPR/Cas9 system consisting of the Cas9 protein, synthetic 102-nt sgRNA or a pair of guide crRNA/tracrRNA, and blocking photocleavable oligodeoxyribonucleotides has been designed.
A photoactivatable CRISPR/Cas9 system composed of Cas9, guide RNA, and blocking photocleavable oligodeoxyribonucleotides was designed.
A photoactivatable CRISPR/Cas9 system consisting of the Cas9 protein, synthetic 102-nt sgRNA or a pair of guide crRNA/tracrRNA, and blocking photocleavable oligodeoxyribonucleotides has been designed.
A photoactivatable CRISPR/Cas9 system composed of Cas9, guide RNA, and blocking photocleavable oligodeoxyribonucleotides was designed.
A photoactivatable CRISPR/Cas9 system consisting of the Cas9 protein, synthetic 102-nt sgRNA or a pair of guide crRNA/tracrRNA, and blocking photocleavable oligodeoxyribonucleotides has been designed.
A photoactivatable CRISPR/Cas9 system composed of Cas9, guide RNA, and blocking photocleavable oligodeoxyribonucleotides was designed.
A photoactivatable CRISPR/Cas9 system consisting of the Cas9 protein, synthetic 102-nt sgRNA or a pair of guide crRNA/tracrRNA, and blocking photocleavable oligodeoxyribonucleotides has been designed.
A photoactivatable CRISPR/Cas9 system composed of Cas9, guide RNA, and blocking photocleavable oligodeoxyribonucleotides was designed.
A photoactivatable CRISPR/Cas9 system consisting of the Cas9 protein, synthetic 102-nt sgRNA or a pair of guide crRNA/tracrRNA, and blocking photocleavable oligodeoxyribonucleotides has been designed.
A photoactivatable CRISPR/Cas9 system composed of Cas9, guide RNA, and blocking photocleavable oligodeoxyribonucleotides was designed.
A photoactivatable CRISPR/Cas9 system consisting of the Cas9 protein, synthetic 102-nt sgRNA or a pair of guide crRNA/tracrRNA, and blocking photocleavable oligodeoxyribonucleotides has been designed.
A photoactivatable CRISPR/Cas9 system composed of Cas9, guide RNA, and blocking photocleavable oligodeoxyribonucleotides was designed.
A photoactivatable CRISPR/Cas9 system consisting of the Cas9 protein, synthetic 102-nt sgRNA or a pair of guide crRNA/tracrRNA, and blocking photocleavable oligodeoxyribonucleotides has been designed.
UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can produce photoactivatable gene editing in vitro.
It has been shown that UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can result in photoactivatable gene editing in vitro.
UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can produce photoactivatable gene editing in vitro.
It has been shown that UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can result in photoactivatable gene editing in vitro.
UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can produce photoactivatable gene editing in vitro.
It has been shown that UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can result in photoactivatable gene editing in vitro.
UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can produce photoactivatable gene editing in vitro.
It has been shown that UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can result in photoactivatable gene editing in vitro.
UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can produce photoactivatable gene editing in vitro.
It has been shown that UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can result in photoactivatable gene editing in vitro.
UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can produce photoactivatable gene editing in vitro.
It has been shown that UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can result in photoactivatable gene editing in vitro.
UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can produce photoactivatable gene editing in vitro.
It has been shown that UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can result in photoactivatable gene editing in vitro.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug photoactivatable-crispr-cas9-system
A photoactivatable CRISPR/Cas9 system consisting of the Cas9 protein, synthetic 102-nt sgRNA or a pair of guide crRNA/tracrRNA, and blocking photocleavable oligodeoxyribonucleotides has been designed.
Source:
designsupports
A photoactivatable CRISPR/Cas9 system composed of Cas9, guide RNA, and blocking photocleavable oligodeoxyribonucleotides was designed.
A photoactivatable CRISPR/Cas9 system consisting of the Cas9 protein, synthetic 102-nt sgRNA or a pair of guide crRNA/tracrRNA, and blocking photocleavable oligodeoxyribonucleotides has been designed.
Source:
functional effectsupports
UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can produce photoactivatable gene editing in vitro.
It has been shown that UV irradiation of the CRISPR/Cas9 system in the presence of photomodified blocking oligodeoxyribonucleotides can result in photoactivatable gene editing in vitro.
Source:
Comparisons
Source-backed strengths
The system was explicitly designed as a multi-component photoactivatable CRISPR/Cas9 configuration and supports either a single-guide RNA format or a crRNA/tracrRNA format. Source claims state that UV irradiation can induce gene editing in vitro in the presence of the photomodified blocking oligodeoxyribonucleotides.
Ranked Citations
- 1.