Source 1primary paper2021International Journal of Molecular Sciences
Toolkit/photoactivatable nanoCRISPR/Cas9 system
photoactivatable nanoCRISPR/Cas9 system
Also known as: nanoCRISPR/Cas9 system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The photoactivatable nanoCRISPR/Cas9 system is a light-gated CRISPR/Cas9 gene-editing platform built from crRNA, auxiliary photocleavable oligodeoxyribonucleotides complementary to the crRNA, and carbon nanoparticles. In this design, crRNA is immobilized in a blocked state before irradiation, and 365 nm UV exposure photocleaves the auxiliary oligonucleotides to release crRNA and restore Cas9 activity.
Usefulness & Problems
Why this is useful
This system provides optical control over CRISPR/Cas9 activity by suppressing guide function before illumination and reactivating it after light exposure. It is useful for applications requiring externally triggered gene-editing activation, with tuning enabled through oligonucleotide design, irradiation conditions, and nanoparticle choice.
Problem solved
It addresses the problem of keeping Cas9 inactive until a defined light stimulus is applied. Specifically, it solves this by reversibly immobilizing crRNA on carbon nanoparticles through complementary photocleavable oligodeoxyribonucleotides and releasing the crRNA after 365 nm irradiation.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
light-triggered crrna releaselight-triggered release of crrnaPhotocleavagereversible crrna immobilizationTechniques
No technique tags yet.
Target processes
editingInput: Light
Implementation Constraints
The construct uses crRNA, photocleavable oligodeoxyribonucleotides complementary to the crRNA, and carbon nanoparticles. Practical performance depends on blocking oligonucleotide length, number of photocleavable linkers, irradiation time, and nanoparticle type; among the tested variants, carbon-encapsulated iron nanoparticles were reported as the most promising.
The supplied evidence is limited to a single source and does not provide detailed quantitative editing outcomes, organismal scope, or independent replication. The system requires 365 nm UV irradiation, and the available evidence does not describe broader validation across targets, cell types, or in vivo settings.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The authors proposed a photoactivatable CRISPR/Cas9 gene-editing system based on photocleavable oligodeoxyribonucleotides complementary to crRNA.
Here, we proposed a new approach to engineering a photoactivatable CRISPR/Cas9 gene-editing system. The novel nanoCRISPR/Cas9 system is based on the use of auxiliary photocleavable oligodeoxyribonucleotides (PC-DNAs) complementary to crRNA.
The authors proposed a photoactivatable CRISPR/Cas9 gene-editing system based on photocleavable oligodeoxyribonucleotides complementary to crRNA.
Here, we proposed a new approach to engineering a photoactivatable CRISPR/Cas9 gene-editing system. The novel nanoCRISPR/Cas9 system is based on the use of auxiliary photocleavable oligodeoxyribonucleotides (PC-DNAs) complementary to crRNA.
The authors proposed a photoactivatable CRISPR/Cas9 gene-editing system based on photocleavable oligodeoxyribonucleotides complementary to crRNA.
Here, we proposed a new approach to engineering a photoactivatable CRISPR/Cas9 gene-editing system. The novel nanoCRISPR/Cas9 system is based on the use of auxiliary photocleavable oligodeoxyribonucleotides (PC-DNAs) complementary to crRNA.
The authors proposed a photoactivatable CRISPR/Cas9 gene-editing system based on photocleavable oligodeoxyribonucleotides complementary to crRNA.
Here, we proposed a new approach to engineering a photoactivatable CRISPR/Cas9 gene-editing system. The novel nanoCRISPR/Cas9 system is based on the use of auxiliary photocleavable oligodeoxyribonucleotides (PC-DNAs) complementary to crRNA.
The authors proposed a photoactivatable CRISPR/Cas9 gene-editing system based on photocleavable oligodeoxyribonucleotides complementary to crRNA.
Here, we proposed a new approach to engineering a photoactivatable CRISPR/Cas9 gene-editing system. The novel nanoCRISPR/Cas9 system is based on the use of auxiliary photocleavable oligodeoxyribonucleotides (PC-DNAs) complementary to crRNA.
The authors proposed a photoactivatable CRISPR/Cas9 gene-editing system based on photocleavable oligodeoxyribonucleotides complementary to crRNA.
Here, we proposed a new approach to engineering a photoactivatable CRISPR/Cas9 gene-editing system. The novel nanoCRISPR/Cas9 system is based on the use of auxiliary photocleavable oligodeoxyribonucleotides (PC-DNAs) complementary to crRNA.
The authors proposed a photoactivatable CRISPR/Cas9 gene-editing system based on photocleavable oligodeoxyribonucleotides complementary to crRNA.
Here, we proposed a new approach to engineering a photoactivatable CRISPR/Cas9 gene-editing system. The novel nanoCRISPR/Cas9 system is based on the use of auxiliary photocleavable oligodeoxyribonucleotides (PC-DNAs) complementary to crRNA.
Immobilizing photocleavable oligonucleotides on carbon nanoparticles blocks crRNA and corresponding Cas9 activity before UV irradiation, and UV irradiation at 365 nm releases crRNA and restores Cas9 activity.
Immobilizing PC-DNAs on the surface of carbon nanoparticles through 3'-terminal pyrene residue provided sufficient blocking of crRNA (and corresponding Cas9 activity) before UV irradiation and allows for crRNA release after UV irradiation at 365 nm, which restores Cas9 activity.
UV irradiation wavelength 365 nm
Immobilizing photocleavable oligonucleotides on carbon nanoparticles blocks crRNA and corresponding Cas9 activity before UV irradiation, and UV irradiation at 365 nm releases crRNA and restores Cas9 activity.
Immobilizing PC-DNAs on the surface of carbon nanoparticles through 3'-terminal pyrene residue provided sufficient blocking of crRNA (and corresponding Cas9 activity) before UV irradiation and allows for crRNA release after UV irradiation at 365 nm, which restores Cas9 activity.
UV irradiation wavelength 365 nm
Immobilizing photocleavable oligonucleotides on carbon nanoparticles blocks crRNA and corresponding Cas9 activity before UV irradiation, and UV irradiation at 365 nm releases crRNA and restores Cas9 activity.
Immobilizing PC-DNAs on the surface of carbon nanoparticles through 3'-terminal pyrene residue provided sufficient blocking of crRNA (and corresponding Cas9 activity) before UV irradiation and allows for crRNA release after UV irradiation at 365 nm, which restores Cas9 activity.
UV irradiation wavelength 365 nm
Immobilizing photocleavable oligonucleotides on carbon nanoparticles blocks crRNA and corresponding Cas9 activity before UV irradiation, and UV irradiation at 365 nm releases crRNA and restores Cas9 activity.
Immobilizing PC-DNAs on the surface of carbon nanoparticles through 3'-terminal pyrene residue provided sufficient blocking of crRNA (and corresponding Cas9 activity) before UV irradiation and allows for crRNA release after UV irradiation at 365 nm, which restores Cas9 activity.
UV irradiation wavelength 365 nm
Immobilizing photocleavable oligonucleotides on carbon nanoparticles blocks crRNA and corresponding Cas9 activity before UV irradiation, and UV irradiation at 365 nm releases crRNA and restores Cas9 activity.
Immobilizing PC-DNAs on the surface of carbon nanoparticles through 3'-terminal pyrene residue provided sufficient blocking of crRNA (and corresponding Cas9 activity) before UV irradiation and allows for crRNA release after UV irradiation at 365 nm, which restores Cas9 activity.
UV irradiation wavelength 365 nm
Immobilizing photocleavable oligonucleotides on carbon nanoparticles blocks crRNA and corresponding Cas9 activity before UV irradiation, and UV irradiation at 365 nm releases crRNA and restores Cas9 activity.
Immobilizing PC-DNAs on the surface of carbon nanoparticles through 3'-terminal pyrene residue provided sufficient blocking of crRNA (and corresponding Cas9 activity) before UV irradiation and allows for crRNA release after UV irradiation at 365 nm, which restores Cas9 activity.
UV irradiation wavelength 365 nm
Immobilizing photocleavable oligonucleotides on carbon nanoparticles blocks crRNA and corresponding Cas9 activity before UV irradiation, and UV irradiation at 365 nm releases crRNA and restores Cas9 activity.
Immobilizing PC-DNAs on the surface of carbon nanoparticles through 3'-terminal pyrene residue provided sufficient blocking of crRNA (and corresponding Cas9 activity) before UV irradiation and allows for crRNA release after UV irradiation at 365 nm, which restores Cas9 activity.
UV irradiation wavelength 365 nm
The authors optimized blocking oligonucleotide length, linker number, irradiation time, and carbon nanoparticle type, and identified the carbon-encapsulated iron nanoparticle version as the most promising because it gave the greatest before-versus-after irradiation functional activity difference.
We optimized the length of blocking photocleavable oligonucleotide, number of linkers, time of irradiation, and the type of carbon nanoparticles. Based on the results, we consider the nanoCRISPR/Cas9 system involving carbon-encapsulated iron nanoparticles the most promising. It provides the greatest difference of functional activity before/after irradiation
The authors optimized blocking oligonucleotide length, linker number, irradiation time, and carbon nanoparticle type, and identified the carbon-encapsulated iron nanoparticle version as the most promising because it gave the greatest before-versus-after irradiation functional activity difference.
We optimized the length of blocking photocleavable oligonucleotide, number of linkers, time of irradiation, and the type of carbon nanoparticles. Based on the results, we consider the nanoCRISPR/Cas9 system involving carbon-encapsulated iron nanoparticles the most promising. It provides the greatest difference of functional activity before/after irradiation
The authors optimized blocking oligonucleotide length, linker number, irradiation time, and carbon nanoparticle type, and identified the carbon-encapsulated iron nanoparticle version as the most promising because it gave the greatest before-versus-after irradiation functional activity difference.
We optimized the length of blocking photocleavable oligonucleotide, number of linkers, time of irradiation, and the type of carbon nanoparticles. Based on the results, we consider the nanoCRISPR/Cas9 system involving carbon-encapsulated iron nanoparticles the most promising. It provides the greatest difference of functional activity before/after irradiation
The authors optimized blocking oligonucleotide length, linker number, irradiation time, and carbon nanoparticle type, and identified the carbon-encapsulated iron nanoparticle version as the most promising because it gave the greatest before-versus-after irradiation functional activity difference.
We optimized the length of blocking photocleavable oligonucleotide, number of linkers, time of irradiation, and the type of carbon nanoparticles. Based on the results, we consider the nanoCRISPR/Cas9 system involving carbon-encapsulated iron nanoparticles the most promising. It provides the greatest difference of functional activity before/after irradiation
The authors optimized blocking oligonucleotide length, linker number, irradiation time, and carbon nanoparticle type, and identified the carbon-encapsulated iron nanoparticle version as the most promising because it gave the greatest before-versus-after irradiation functional activity difference.
We optimized the length of blocking photocleavable oligonucleotide, number of linkers, time of irradiation, and the type of carbon nanoparticles. Based on the results, we consider the nanoCRISPR/Cas9 system involving carbon-encapsulated iron nanoparticles the most promising. It provides the greatest difference of functional activity before/after irradiation
The authors optimized blocking oligonucleotide length, linker number, irradiation time, and carbon nanoparticle type, and identified the carbon-encapsulated iron nanoparticle version as the most promising because it gave the greatest before-versus-after irradiation functional activity difference.
We optimized the length of blocking photocleavable oligonucleotide, number of linkers, time of irradiation, and the type of carbon nanoparticles. Based on the results, we consider the nanoCRISPR/Cas9 system involving carbon-encapsulated iron nanoparticles the most promising. It provides the greatest difference of functional activity before/after irradiation
The authors optimized blocking oligonucleotide length, linker number, irradiation time, and carbon nanoparticle type, and identified the carbon-encapsulated iron nanoparticle version as the most promising because it gave the greatest before-versus-after irradiation functional activity difference.
We optimized the length of blocking photocleavable oligonucleotide, number of linkers, time of irradiation, and the type of carbon nanoparticles. Based on the results, we consider the nanoCRISPR/Cas9 system involving carbon-encapsulated iron nanoparticles the most promising. It provides the greatest difference of functional activity before/after irradiation
The carbon-encapsulated iron nanoparticle nanoCRISPR/Cas9 system could prospectively support magnetic field-controlled delivery and UV-induced spatiotemporal gene editing.
and can be used in prospective for magnetic field-controlled delivery of CRISPR system into the target cells or tissues and spatiotemporal gene editing induced by UV irradiation.
The carbon-encapsulated iron nanoparticle nanoCRISPR/Cas9 system could prospectively support magnetic field-controlled delivery and UV-induced spatiotemporal gene editing.
and can be used in prospective for magnetic field-controlled delivery of CRISPR system into the target cells or tissues and spatiotemporal gene editing induced by UV irradiation.
The carbon-encapsulated iron nanoparticle nanoCRISPR/Cas9 system could prospectively support magnetic field-controlled delivery and UV-induced spatiotemporal gene editing.
and can be used in prospective for magnetic field-controlled delivery of CRISPR system into the target cells or tissues and spatiotemporal gene editing induced by UV irradiation.
The carbon-encapsulated iron nanoparticle nanoCRISPR/Cas9 system could prospectively support magnetic field-controlled delivery and UV-induced spatiotemporal gene editing.
and can be used in prospective for magnetic field-controlled delivery of CRISPR system into the target cells or tissues and spatiotemporal gene editing induced by UV irradiation.
The carbon-encapsulated iron nanoparticle nanoCRISPR/Cas9 system could prospectively support magnetic field-controlled delivery and UV-induced spatiotemporal gene editing.
and can be used in prospective for magnetic field-controlled delivery of CRISPR system into the target cells or tissues and spatiotemporal gene editing induced by UV irradiation.
The carbon-encapsulated iron nanoparticle nanoCRISPR/Cas9 system could prospectively support magnetic field-controlled delivery and UV-induced spatiotemporal gene editing.
and can be used in prospective for magnetic field-controlled delivery of CRISPR system into the target cells or tissues and spatiotemporal gene editing induced by UV irradiation.
The carbon-encapsulated iron nanoparticle nanoCRISPR/Cas9 system could prospectively support magnetic field-controlled delivery and UV-induced spatiotemporal gene editing.
and can be used in prospective for magnetic field-controlled delivery of CRISPR system into the target cells or tissues and spatiotemporal gene editing induced by UV irradiation.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug photoactivatable-nanocrispr-cas9-system
Here, we proposed a new approach to engineering a photoactivatable CRISPR/Cas9 gene-editing system. The novel nanoCRISPR/Cas9 system is based on the use of auxiliary photocleavable oligodeoxyribonucleotides (PC-DNAs) complementary to crRNA.
Source:
engineering approachsupports
The authors proposed a photoactivatable CRISPR/Cas9 gene-editing system based on photocleavable oligodeoxyribonucleotides complementary to crRNA.
Here, we proposed a new approach to engineering a photoactivatable CRISPR/Cas9 gene-editing system. The novel nanoCRISPR/Cas9 system is based on the use of auxiliary photocleavable oligodeoxyribonucleotides (PC-DNAs) complementary to crRNA.
Source:
mechanism of controlsupports
Immobilizing photocleavable oligonucleotides on carbon nanoparticles blocks crRNA and corresponding Cas9 activity before UV irradiation, and UV irradiation at 365 nm releases crRNA and restores Cas9 activity.
Immobilizing PC-DNAs on the surface of carbon nanoparticles through 3'-terminal pyrene residue provided sufficient blocking of crRNA (and corresponding Cas9 activity) before UV irradiation and allows for crRNA release after UV irradiation at 365 nm, which restores Cas9 activity.
Source:
optimization resultsupports
The authors optimized blocking oligonucleotide length, linker number, irradiation time, and carbon nanoparticle type, and identified the carbon-encapsulated iron nanoparticle version as the most promising because it gave the greatest before-versus-after irradiation functional activity difference.
We optimized the length of blocking photocleavable oligonucleotide, number of linkers, time of irradiation, and the type of carbon nanoparticles. Based on the results, we consider the nanoCRISPR/Cas9 system involving carbon-encapsulated iron nanoparticles the most promising. It provides the greatest difference of functional activity before/after irradiation
Source:
prospective applicationsupports
The carbon-encapsulated iron nanoparticle nanoCRISPR/Cas9 system could prospectively support magnetic field-controlled delivery and UV-induced spatiotemporal gene editing.
and can be used in prospective for magnetic field-controlled delivery of CRISPR system into the target cells or tissues and spatiotemporal gene editing induced by UV irradiation.
Source:
Comparisons
Source-backed strengths
The reported design directly couples light input to Cas9 activation through a defined crRNA-blocking and release mechanism. The authors also optimized blocking oligonucleotide length, photocleavable linker number, irradiation time, and carbon nanoparticle type, and identified carbon-encapsulated iron nanoparticles as giving the largest pre- versus post-irradiation functional activity difference.
Source:
Here, we proposed a new approach to engineering a photoactivatable CRISPR/Cas9 gene-editing system. The novel nanoCRISPR/Cas9 system is based on the use of auxiliary photocleavable oligodeoxyribonucleotides (PC-DNAs) complementary to crRNA.
Source:
We optimized the length of blocking photocleavable oligonucleotide, number of linkers, time of irradiation, and the type of carbon nanoparticles. Based on the results, we consider the nanoCRISPR/Cas9 system involving carbon-encapsulated iron nanoparticles the most promising. It provides the greatest difference of functional activity before/after irradiation
Ranked Citations
- 1.