Source 1primary paper2015ChemBioChem
Toolkit/photocaged arabinose
photocaged arabinose
Also known as: caged arabinose, photocaged arabinose compounds
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Photocaged arabinose comprises two one-step cleavable arabinose derivatives used as light-sensitive inducers of transcription in bacteria. Upon UV-A illumination, these compounds undergo photocleavage to release active inducer and trigger arabinose-regulated gene expression with rapid and gradual control.
Usefulness & Problems
Why this is useful
This tool provides optical control over bacterial transcription without requiring a genetically encoded photoreceptor, using small-molecule inducers that can be activated by light. It was shown to support light-triggered expression of both GFP and the complete violacein biosynthetic pathway, indicating utility for reporter expression and pathway-level regulation.
Source:
as demonstrated for GFP and the complete violacein biosynthetic pathway
Source:
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Problem solved
Photocaged arabinose addresses the problem of achieving rapid, precise, and homogeneous light-dependent control of arabinose-inducible transcription in bacteria. It enables external activation of gene expression by UV-A exposure rather than immediate addition of active arabinose.
Source:
as demonstrated for GFP and the complete violacein biosynthetic pathway
Problem links
Need precise spatiotemporal control with light input
DerivedPhotocaged arabinose comprises two one-step cleavable arabinose derivatives that function as light-sensitive inducers of transcription in bacteria. Upon UV-A illumination, these compounds release active inducer and enable rapid, gradual, and precise control of arabinose-regulated gene expression.
Need tighter control over gene expression timing or amplitude
DerivedPhotocaged arabinose comprises two one-step cleavable arabinose derivatives that function as light-sensitive inducers of transcription in bacteria. Upon UV-A illumination, these compounds release active inducer and enable rapid, gradual, and precise control of arabinose-regulated gene expression.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
transcriptionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: cleavageswitch architecture: multi component
Implementation requires addition of photocaged arabinose compounds to bacterial systems regulated by arabinose-responsive transcription and subsequent UV-A illumination to release the active inducer. The evidence supports use as a multi-component switch based on an exogenous small molecule plus light input, but does not provide further construct design, dosing, or delivery details.
The supplied evidence is limited to a single 2015 study and does not provide independent replication. The available text does not specify the exact chemical structures, uncaging wavelengths beyond UV-A, host range beyond bacteria, or quantitative performance metrics such as dynamic range, leakiness, or phototoxicity.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug photocaged-arabinose
we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria
Source:
application scopesupports
Photocaged arabinose enabled light-triggered expression as demonstrated with GFP and the complete violacein biosynthetic pathway.
as demonstrated for GFP and the complete violacein biosynthetic pathway
Source:
light activated expression controlsupports
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production.
Exposure of caged arabinose to UV-A light resulted in rapid activation of protein production
Source:
precision controlsupports
Photocaged arabinose enabled precise and homogeneous control of bacterial target gene expression.
We have thus established a novel phototrigger for synthetic bio(techno)logy applications that enables precise and homogeneous control of bacterial target gene expression.
Source:
single cell expression homogeneitysupports
Single-cell analysis indicated that photocaged arabinose overcame intrinsic heterogeneity of arabinose-mediated induction of gene expression.
single-cell analysis revealed that intrinsic heterogeneity of arabinose-mediated induction of gene expression was overcome when using photocaged arabinose
Source:
tool introductionsupports
Two novel one-step cleavable photocaged arabinose compounds were reported as light-sensitive inducers of transcription in bacteria.
Here, we report on two novel one-step cleavable photocaged arabinose compounds, which were applied as light-sensitive inducers of transcription in bacteria.
Source:
Comparisons
Source-backed strengths
The reported compounds are one-step cleavable and produced rapid activation of protein production after UV-A illumination. The study also reported gradual, precise, and homogeneous control of bacterial target gene expression, with demonstrations spanning GFP expression and a full biosynthetic pathway.
Compared with CRY2-CIB1 light-inducible transcription system
photocaged arabinose and CRY2-CIB1 light-inducible transcription system address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; same primary input modality: light
Compared with light-switchable transcription factors
photocaged arabinose and light-switchable transcription factors address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; same primary input modality: light
Compared with mOptoT7
photocaged arabinose and mOptoT7 address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.