Source 1primary paper2016Applied and Environmental Microbiology
Toolkit/photocaged IPTG
photocaged IPTG
Also known as: cIPTG, photocaged isopropyl-b2-d-1-thiogalactopyranoside, photocaged isopropyl-b2-D-thiogalactopyranoside
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Photocaged IPTG (cIPTG) is a light-activated synthetic inducer used with lac promoter-based bacterial expression systems. Illumination uncages the compound, enabling light-mediated derepression of lac-controlled transcription, and the approach has been applied in bacteria including Rhodobacter capsulatus and Corynebacterium glutamicum.
Usefulness & Problems
Why this is useful
This tool provides optochemical control of bacterial gene expression with light rather than constitutive chemical induction alone. The supplied evidence indicates that it supports noninvasive, spatiotemporally controlled induction and can be used to engineer cellular functions such as intrinsic carotenoid biosynthesis in Rhodobacter capsulatus.
Source:
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Source:
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
Source:
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Source:
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Problem solved
The evidence states that cIPTG addresses drawbacks associated with conventional IPTG-mediated induction in lac-based systems, including poor inducibility and phenotypic heterogeneity reported in Corynebacterium glutamicum. In the cited study, these drawbacks could be almost completely abolished by applying photocaged IPTG as a synthetic inducer.
Source:
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Source:
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
Source:
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Source:
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Published Workflows
Objective: Implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using photocaged IPTG variants and demonstrate control of a cellular function.
Why it works: The workflow uses photocaged IPTG as a light-responsive inducer so that illumination can trigger target gene expression, and then applies the same approach to a native cellular function as a demonstration.
light-mediated uncaging of inducer to activate gene expressioninduction of intrinsic carotenoid biosynthesistesting different photocaged inducer variantsoptochemical induction
Stages
- 1.Evaluation of different cIPTG variants in Rhodobacter capsulatus(broad_screen)
The study tested different cIPTG variants to identify a variant that works as a light-mediated on-switch in Rhodobacter capsulatus.
Selection: Ability of cIPTG variants to support light-mediated induction of target gene expression under phototrophic and non-phototrophic cultivation conditions.
- 2.Identification of especially applicable NP-cIPTG variant(hit_picking)
This stage narrows the tested cIPTG variants to the variant highlighted as especially applicable in the host.
Selection: NP-cIPTG was identified as especially applicable for light-mediated induction of target gene expression in Rhodobacter capsulatus.
- 3.Functional demonstration via induction of intrinsic carotenoid biosynthesis(confirmatory_validation)
The stage confirms that the optochemical induction approach can be used not only for target gene expression but also to engineer a cellular function.
Selection: Successful induction of intrinsic carotenoid biosynthesis as a showcase of engineering a cellular function.
Objective: Optimize lac promoter-based gene expression and (+)-valencene biosynthesis in Corynebacterium glutamicum using light-mediated induction with photocaged IPTG.
Why it works: The abstract argues that photocaged IPTG enables noninvasive, spatiotemporal light induction, which in turn provides more precise and homogeneous control than conventional IPTG-mediated induction in Corynebacterium glutamicum.
light-mediated activation of a synthetic inducer for lac promoter-based gene expressionphotocaged inducer-based controllight induction
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
light-triggered chemical uncagingPhotocleavagetranscriptional induction via lac promoter derepressionTechniques
No technique tags yet.
Target processes
recombinationInput: Light
Implementation Constraints
Use of this tool requires a lac promoter-based expression system and light exposure to activate the inducer by uncaging. The evidence supports implementation as a synthetic small-molecule inducer in bacterial hosts, but the supplied text does not specify construct architecture, illumination parameters, or formulation details.
The provided evidence does not report quantitative performance metrics such as induction fold, uncaging efficiency, response time, or wavelength dependence. It also does not establish that cIPTG resolves all limitations of light-controlled expression or broader production bottlenecks in biotechnological applications.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2022MED
Ranked Claims
Photocaged IPTG is a well-established optochemical tool for light-regulated gene expression in bacteria.
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Photocaged IPTG is a well-established optochemical tool for light-regulated gene expression in bacteria.
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Photocaged IPTG is a well-established optochemical tool for light-regulated gene expression in bacteria.
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Photocaged IPTG is a well-established optochemical tool for light-regulated gene expression in bacteria.
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Photocaged IPTG is a well-established optochemical tool for light-regulated gene expression in bacteria.
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Photocaged IPTG is a well-established optochemical tool for light-regulated gene expression in bacteria.
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Photocaged IPTG is a well-established optochemical tool for light-regulated gene expression in bacteria.
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
Photocaged IPTG is a well-established optochemical tool for light-regulated gene expression in bacteria.
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
The optochemical approach was successfully used to induce intrinsic carotenoid biosynthesis in Rhodobacter capsulatus as a demonstration of engineering a cellular function.
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
The optochemical approach was successfully used to induce intrinsic carotenoid biosynthesis in Rhodobacter capsulatus as a demonstration of engineering a cellular function.
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
The optochemical approach was successfully used to induce intrinsic carotenoid biosynthesis in Rhodobacter capsulatus as a demonstration of engineering a cellular function.
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
The optochemical approach was successfully used to induce intrinsic carotenoid biosynthesis in Rhodobacter capsulatus as a demonstration of engineering a cellular function.
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
The optochemical approach was successfully used to induce intrinsic carotenoid biosynthesis in Rhodobacter capsulatus as a demonstration of engineering a cellular function.
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
The optochemical approach was successfully used to induce intrinsic carotenoid biosynthesis in Rhodobacter capsulatus as a demonstration of engineering a cellular function.
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
The optochemical approach was successfully used to induce intrinsic carotenoid biosynthesis in Rhodobacter capsulatus as a demonstration of engineering a cellular function.
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
The optochemical approach was successfully used to induce intrinsic carotenoid biosynthesis in Rhodobacter capsulatus as a demonstration of engineering a cellular function.
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
Among the tested cIPTG variants, 6-nitropiperonyl-(NP)-cIPTG was especially applicable for light-mediated induction of target gene expression in Rhodobacter capsulatus.
We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
Among the tested cIPTG variants, 6-nitropiperonyl-(NP)-cIPTG was especially applicable for light-mediated induction of target gene expression in Rhodobacter capsulatus.
We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
Among the tested cIPTG variants, 6-nitropiperonyl-(NP)-cIPTG was especially applicable for light-mediated induction of target gene expression in Rhodobacter capsulatus.
We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
Among the tested cIPTG variants, 6-nitropiperonyl-(NP)-cIPTG was especially applicable for light-mediated induction of target gene expression in Rhodobacter capsulatus.
We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
Among the tested cIPTG variants, 6-nitropiperonyl-(NP)-cIPTG was especially applicable for light-mediated induction of target gene expression in Rhodobacter capsulatus.
We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
Among the tested cIPTG variants, 6-nitropiperonyl-(NP)-cIPTG was especially applicable for light-mediated induction of target gene expression in Rhodobacter capsulatus.
We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
Among the tested cIPTG variants, 6-nitropiperonyl-(NP)-cIPTG was especially applicable for light-mediated induction of target gene expression in Rhodobacter capsulatus.
We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
Photocaged IPTG is presented as a light-responsive tool with promising properties for automated multi-factorial control of cellular functions and optimization of production processes.
Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.
Photocaged IPTG is presented as a light-responsive tool with promising properties for automated multi-factorial control of cellular functions and optimization of production processes.
Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.
Photocaged IPTG is presented as a light-responsive tool with promising properties for automated multi-factorial control of cellular functions and optimization of production processes.
Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.
Photocaged IPTG is presented as a light-responsive tool with promising properties for automated multi-factorial control of cellular functions and optimization of production processes.
Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.
Photocaged IPTG is presented as a light-responsive tool with promising properties for automated multi-factorial control of cellular functions and optimization of production processes.
Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.
Photocaged IPTG is presented as a light-responsive tool with promising properties for automated multi-factorial control of cellular functions and optimization of production processes.
Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.
Photocaged IPTG is presented as a light-responsive tool with promising properties for automated multi-factorial control of cellular functions and optimization of production processes.
Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.
Photocaged IPTG is presented as a light-responsive tool with promising properties for automated multi-factorial control of cellular functions and optimization of production processes.
Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.
The study aimed to implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using different cIPTG variants under phototrophic and non-phototrophic conditions.
In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions.
The study aimed to implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using different cIPTG variants under phototrophic and non-phototrophic conditions.
In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions.
The study aimed to implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using different cIPTG variants under phototrophic and non-phototrophic conditions.
In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions.
The study aimed to implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using different cIPTG variants under phototrophic and non-phototrophic conditions.
In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions.
The study aimed to implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using different cIPTG variants under phototrophic and non-phototrophic conditions.
In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions.
The study aimed to implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using different cIPTG variants under phototrophic and non-phototrophic conditions.
In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions.
The study aimed to implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using different cIPTG variants under phototrophic and non-phototrophic conditions.
In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions.
The study aimed to implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using different cIPTG variants under phototrophic and non-phototrophic conditions.
In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions.
For increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction provides precise, homogeneous, higher-order control that could help automate or optimize future biotechnological applications.
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
For increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction provides precise, homogeneous, higher-order control that could help automate or optimize future biotechnological applications.
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
For increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction provides precise, homogeneous, higher-order control that could help automate or optimize future biotechnological applications.
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
For increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction provides precise, homogeneous, higher-order control that could help automate or optimize future biotechnological applications.
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
For increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction provides precise, homogeneous, higher-order control that could help automate or optimize future biotechnological applications.
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
For increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction provides precise, homogeneous, higher-order control that could help automate or optimize future biotechnological applications.
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
For increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction provides precise, homogeneous, higher-order control that could help automate or optimize future biotechnological applications.
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
The study concerns light-mediated optimization of (+)-valencene biosynthesis in Corynebacterium glutamicum.
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-b2- <scp>d</scp> -Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum
The study concerns light-mediated optimization of (+)-valencene biosynthesis in Corynebacterium glutamicum.
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-b2- <scp>d</scp> -Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum
The study concerns light-mediated optimization of (+)-valencene biosynthesis in Corynebacterium glutamicum.
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-b2- <scp>d</scp> -Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum
The study concerns light-mediated optimization of (+)-valencene biosynthesis in Corynebacterium glutamicum.
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-b2- <scp>d</scp> -Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum
The study concerns light-mediated optimization of (+)-valencene biosynthesis in Corynebacterium glutamicum.
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-b2- <scp>d</scp> -Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum
The study concerns light-mediated optimization of (+)-valencene biosynthesis in Corynebacterium glutamicum.
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-b2- <scp>d</scp> -Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum
The study concerns light-mediated optimization of (+)-valencene biosynthesis in Corynebacterium glutamicum.
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-b2- <scp>d</scp> -Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
Approval Evidence
2 sources9 linked approval claimsfirst-pass slug photocaged-iptg
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
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By applying photocaged IPTG as a synthetic inducer
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By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
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application claimsupports
Photocaged IPTG is a well-established optochemical tool for light-regulated gene expression in bacteria.
Photocaged inducer molecules, especially photocaged isopropyl-b2-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression
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application demo claimsupports
The optochemical approach was successfully used to induce intrinsic carotenoid biosynthesis in Rhodobacter capsulatus as a demonstration of engineering a cellular function.
Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function.
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future use claimsupports
Photocaged IPTG is presented as a light-responsive tool with promising properties for automated multi-factorial control of cellular functions and optimization of production processes.
Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.
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implementation claimsupports
The study aimed to implement a light-mediated on-switch for target gene expression in Rhodobacter capsulatus using different cIPTG variants under phototrophic and non-phototrophic conditions.
In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions.
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application potentialsupports
For increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction provides precise, homogeneous, higher-order control that could help automate or optimize future biotechnological applications.
Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
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application potentialsupports
Light-controlled gene expression has strong potential for synthetic biotechnological applications and can provide precise, homogeneous, noninvasive, and spatiotemporal control for parallelized expression cultures.
Specifically, light-controlled gene expression exhibits an enormous potential for various synthetic bio(techno)logical purposes. Especially for increasing numbers of parallelized expression cultures, noninvasive and spatiotemporal light induction qualifies for a precise, homogeneous, and thus higher-order control to fully automatize or optimize future biotechnological applications.
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biosynthesis applicationsupports
The study concerns light-mediated optimization of (+)-valencene biosynthesis in Corynebacterium glutamicum.
Light-Controlled Cell Factories: Employing Photocaged Isopropyl-b2- <scp>d</scp> -Thiogalactopyranoside for Light-Mediated Optimization of lac Promoter-Based Gene Expression and (+)-Valencene Biosynthesis in Corynebacterium glutamicum
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performance improvementsupports
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
Source:
performance improvementsupports
In Corynebacterium glutamicum, applying photocaged IPTG as a synthetic inducer could almost completely abolish poor inducibility and phenotypic heterogeneity associated with IPTG-mediated induction of lac-based gene expression.
Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
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Comparisons
Source-backed strengths
Photocaged IPTG is described as a well-established optochemical tool for light-regulated gene expression in bacteria. It has been demonstrated in at least two bacterial contexts from the supplied citations, including control of gene expression in Rhodobacter capsulatus and optimization of lac promoter-based expression linked to metabolic engineering in Corynebacterium glutamicum.
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We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium.
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Before our study, poor inducibility, together with phenotypic heterogeneity, was reported for the IPTG-mediated induction of lac-based gene expression in Corynebacterium glutamicum By applying photocaged IPTG as a synthetic inducer, however, these drawbacks could be almost completely abolished.
Ranked Citations
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