Source 1primary paper2020Analytical Chemistry
Toolkit/photocontrollable nucleic acid cascade recycling amplification
photocontrollable nucleic acid cascade recycling amplification
Also known as: NIR light activatable signal amplification
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Photocontrollable nucleic acid cascade recycling amplification is a near-infrared light-activatable nucleic acid signal amplification strategy. It combines a photocontrollable nucleic acid displacement reaction, exonuclease III-assisted cascade recycling amplification, and upconversion nanoparticles to trigger signal-amplified mRNA imaging with spatiotemporal control in living cancer cells.
Usefulness & Problems
Why this is useful
This method is useful because it enables external near-infrared light control over when and where nucleic acid amplification occurs. The reported application is spatiotemporally controllable, signal-amplified mRNA imaging in selected living cancer cells.
Source:
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
Problem solved
It addresses the problem of triggering nucleic acid signal amplification in a controllable manner rather than constitutively. The cited study specifically positions it as a solution for spatiotemporally controllable mRNA imaging in living cells.
Source:
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Mechanisms
exonuclease iii-assisted cascade recycling amplificationnear-infrared light activationnucleic acid strand displacementphotocontrol by near-infrared light activationphotocontrol of nucleic acid reactionsupconversion nanoparticle-mediated light conversionTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The method is achieved by integrating three components: a photocontrollable nucleic acid displacement reaction, exonuclease III-assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles. Near-infrared light is the triggering input, and the reported use case is mRNA imaging in living cancer cells.
The available evidence comes from a conceptual study and a single cited report, with validation described only in selected living cancer cells. Quantitative performance metrics, target scope, generality across cell types, and comparative benchmarking are not provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug photocontrollable-nucleic-acid-cascade-recycling-amplification
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
Source:
application demosupports
The developed NIR light triggered signal amplification process was demonstrated in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
As a proof of concept, we demonstrate this developed NIR light triggered signal amplification process in selected living cancer cells for spatiotemporally controllable signal amplified mRNA imaging.
Source:
method compositionsupports
The NIR-activatable amplification strategy is achieved by integrating a photocontrollable nucleic acid displacement reaction, exonuclease III assisted nucleic acid cascade recycling amplification, and upconversion nanoparticles.
This strategy is achieved by integrating photocontrollable nucleic acid displacement reaction with exonuclease III (EXO III) assisted nucleic acid cascade recycling amplification and combination with upconversion nanoparticles (UCNPs), thus resulting in a NIR light activatable signal amplification.
Source:
method introductionsupports
The paper presents a photocontrollable nucleic acid cascade recycling amplification strategy that uses near-infrared light to control and trigger the amplification process.
Herein, we present a conceptual study termed as photocontrollable nucleic acid cascade recycling amplification which uses near-infrared (NIR) light to precisely control and trigger the whole process.
Source:
Comparisons
Source-backed strengths
The strategy is explicitly designed to use near-infrared light to precisely control and trigger the whole amplification process. It was demonstrated in selected living cancer cells, supporting feasibility for signal-amplified mRNA imaging in a cellular context.
Ranked Citations
- 1.