Source 1primary paper2021PLoS Genetics
Toolkit/photosensitive degron
photosensitive degron
Also known as: psd
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The photosensitive degron (psd) is a protein-domain tool that confers acute light-induced degradation on fused proteins. Available evidence indicates activity in the nervous system and a construct requirement that the psd be placed at the carboxy terminus of the target protein.
Usefulness & Problems
Why this is useful
psd is useful for temporally precise reduction of protein abundance using light rather than constitutive genetic perturbation. The supplied evidence specifically supports acute protein degradation in the nervous system, where rapid control of protein levels can be experimentally valuable.
Source:
A photosensitive degron enables acute light-induced protein degradation in the nervous system
Problem solved
psd helps address the problem of inducing rapid, light-triggered loss of a protein of interest in living systems. The evidence also suggests that simple deployment may not be universally effective across contexts, because little effect was reported on Cactus-dependent phenotypes in illuminated developing embryos.
Problem links
Need conditional protein clearance
DerivedThe photosensitive degron (psd) is a protein-domain tool for light-induced protein degradation. Available evidence indicates that it can enable acute degradation in the nervous system and that it must be positioned at the carboxy terminus of the fused target protein.
Need precise spatiotemporal control with light input
DerivedThe photosensitive degron (psd) is a protein-domain tool for light-induced protein degradation. Available evidence indicates that it can enable acute degradation in the nervous system and that it must be positioned at the carboxy terminus of the fused target protein.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Techniques
No technique tags yet.
Target processes
degradationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: single chain
The available evidence indicates that psd must be fused at the carboxy terminus of the associated target protein. No additional implementation details such as chromophore requirements, expression system, linker design, or delivery modality are provided in the supplied evidence.
Evidence indicates that psd has little effect on Cactus-dependent phenotypes during illumination of developing embryos, suggesting context-dependent performance. The supplied material does not provide quantitative degradation kinetics, wavelength requirements, dynamic range, or molecular degradation pathway details.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2015Current Biology
Ranked Claims
The photosensitive degron (psd) has little effect on Cactus-dependent phenotypes in response to illumination of developing embryos.
another previously described photosensitive degron (psd), which also must be located at the carboxy terminus of associated proteins, has little effect on Cactus-dependent phenotypes in response to illumination of developing embryos.
A photosensitive degron enables acute light-induced protein degradation in the nervous system.
A photosensitive degron enables acute light-induced protein degradation in the nervous system
Approval Evidence
2 sources2 linked approval claimsfirst-pass slug photosensitive-degron
another previously described photosensitive degron (psd), which also must be located at the carboxy terminus of associated proteins
Source:
A photosensitive degron enables acute light-induced protein degradation in the nervous system
Source:
comparative effectivenesssupports
The photosensitive degron (psd) has little effect on Cactus-dependent phenotypes in response to illumination of developing embryos.
another previously described photosensitive degron (psd), which also must be located at the carboxy terminus of associated proteins, has little effect on Cactus-dependent phenotypes in response to illumination of developing embryos.
Source:
capabilitysupports
A photosensitive degron enables acute light-induced protein degradation in the nervous system.
A photosensitive degron enables acute light-induced protein degradation in the nervous system
Source:
Comparisons
Source-backed strengths
The strongest supported feature is acute light-induced protein degradation in the nervous system. The tool is also described as a defined fusion domain with a specific positional rule, namely carboxy-terminal placement on associated proteins.
Source:
another previously described photosensitive degron (psd), which also must be located at the carboxy terminus of associated proteins, has little effect on Cactus-dependent phenotypes in response to illumination of developing embryos.
Compared with Jalpha helix
photosensitive degron and Jalpha helix address a similar problem space because they share degradation.
Shared frame: same top-level item type; shared target processes: degradation; shared mechanisms: degradation; same primary input modality: light
Strengths here: appears more independently replicated; looks easier to implement in practice.
Compared with LOV2 domain-based optogenetic tool
photosensitive degron and LOV2 domain-based optogenetic tool address a similar problem space because they share degradation.
Shared frame: same top-level item type; shared target processes: degradation; shared mechanisms: degradation; same primary input modality: light
Strengths here: appears more independently replicated; looks easier to implement in practice.
Compared with photo-N-degron
photosensitive degron and photo-N-degron address a similar problem space because they share degradation.
Shared frame: same top-level item type; shared target processes: degradation; shared mechanisms: degradation; same primary input modality: light
Strengths here: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.
Derived from 1 linked claims. Example evidence: another previously described photosensitive degron (psd), which also must be located at the carboxy terminus of associated proteins, has little effect on Cactus-dependent phenotypes in response to illumination of developing embryos.
- 2.