Source 1primary paper2019
Toolkit/PhyB-CreC
PhyB-CreC
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
PhyB-CreC is the PhyB-fused C-terminal half of Cre recombinase in the CreLite optogenetic split-Cre system. In the presence of phycocyanobilin and 660 nm red light, it associates with PIF6-CreN to reconstitute Cre activity and drive Cre/loxP-dependent recombination in zebrafish embryos.
Usefulness & Problems
Why this is useful
This component enables red-light control of Cre recombinase when paired with PIF6-CreN and PCB, providing inducible recombination with optical input. It was used in developing zebrafish embryos to trigger Cre-dependent fluorescent reporter conversion in heart, skeletal muscle, and epithelium.
Source:
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
Source:
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
Source:
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
Problem solved
PhyB-CreC helps solve the problem of making Cre/loxP recombination conditional on an external light stimulus rather than constitutive Cre expression. The reported system specifically addresses optically induced recombination in vivo using red light and a split-enzyme design.
Source:
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
Source:
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
recombinationInput: Light
Implementation Constraints
PhyB-CreC is implemented as a fusion between PhyB and the inactive C-terminal fragment of split Cre recombinase. Reported activity requires co-delivery with PIF6-CreN, exposure to 660 nm red light, and the presence of phycocyanobilin; the cited application used injected CreLite mRNAs in developing zebrafish embryos.
The available evidence describes PhyB-CreC only as one component of a two-part system and does not support standalone activity. Validation in the supplied evidence is limited to zebrafish embryos and requires both the partner fusion PIF6-CreN and the chromophore PCB.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
In transgenic zebrafish embryos carrying a Cre-dependent multi-color fluorescent reporter, red-light exposure after injection with CreLite mRNAs and PCB resulted in Cre activity measured by multi-spectral cell labeling in heart, skeletal muscle, and epithelium.
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
In transgenic zebrafish embryos carrying a Cre-dependent multi-color fluorescent reporter, red-light exposure after injection with CreLite mRNAs and PCB resulted in Cre activity measured by multi-spectral cell labeling in heart, skeletal muscle, and epithelium.
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
In transgenic zebrafish embryos carrying a Cre-dependent multi-color fluorescent reporter, red-light exposure after injection with CreLite mRNAs and PCB resulted in Cre activity measured by multi-spectral cell labeling in heart, skeletal muscle, and epithelium.
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
In transgenic zebrafish embryos carrying a Cre-dependent multi-color fluorescent reporter, red-light exposure after injection with CreLite mRNAs and PCB resulted in Cre activity measured by multi-spectral cell labeling in heart, skeletal muscle, and epithelium.
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
In transgenic zebrafish embryos carrying a Cre-dependent multi-color fluorescent reporter, red-light exposure after injection with CreLite mRNAs and PCB resulted in Cre activity measured by multi-spectral cell labeling in heart, skeletal muscle, and epithelium.
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
In transgenic zebrafish embryos carrying a Cre-dependent multi-color fluorescent reporter, red-light exposure after injection with CreLite mRNAs and PCB resulted in Cre activity measured by multi-spectral cell labeling in heart, skeletal muscle, and epithelium.
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
In transgenic zebrafish embryos carrying a Cre-dependent multi-color fluorescent reporter, red-light exposure after injection with CreLite mRNAs and PCB resulted in Cre activity measured by multi-spectral cell labeling in heart, skeletal muscle, and epithelium.
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
CreLite disables Cre by splitting Cre and fusing the inactive halves to the red light-inducible binding partners PhyB and PIF6.
Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6.
CreLite disables Cre by splitting Cre and fusing the inactive halves to the red light-inducible binding partners PhyB and PIF6.
Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6.
CreLite disables Cre by splitting Cre and fusing the inactive halves to the red light-inducible binding partners PhyB and PIF6.
Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6.
CreLite disables Cre by splitting Cre and fusing the inactive halves to the red light-inducible binding partners PhyB and PIF6.
Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6.
CreLite disables Cre by splitting Cre and fusing the inactive halves to the red light-inducible binding partners PhyB and PIF6.
Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6.
CreLite disables Cre by splitting Cre and fusing the inactive halves to the red light-inducible binding partners PhyB and PIF6.
Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6.
CreLite disables Cre by splitting Cre and fusing the inactive halves to the red light-inducible binding partners PhyB and PIF6.
Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6.
Red light at 660 nm in the presence of PCB brings PhyB-CreC and PIF6-CreN together to restore Cre activity.
Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity.
activation wavelength 660 nm
Red light at 660 nm in the presence of PCB brings PhyB-CreC and PIF6-CreN together to restore Cre activity.
Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity.
activation wavelength 660 nm
Red light at 660 nm in the presence of PCB brings PhyB-CreC and PIF6-CreN together to restore Cre activity.
Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity.
activation wavelength 660 nm
Red light at 660 nm in the presence of PCB brings PhyB-CreC and PIF6-CreN together to restore Cre activity.
Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity.
activation wavelength 660 nm
Red light at 660 nm in the presence of PCB brings PhyB-CreC and PIF6-CreN together to restore Cre activity.
Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity.
activation wavelength 660 nm
Red light at 660 nm in the presence of PCB brings PhyB-CreC and PIF6-CreN together to restore Cre activity.
Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity.
activation wavelength 660 nm
Red light at 660 nm in the presence of PCB brings PhyB-CreC and PIF6-CreN together to restore Cre activity.
Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity.
activation wavelength 660 nm
CreLite is an optogenetically controlled Cre system that uses red light in developing zebrafish embryos.
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
CreLite is an optogenetically controlled Cre system that uses red light in developing zebrafish embryos.
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
CreLite is an optogenetically controlled Cre system that uses red light in developing zebrafish embryos.
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
CreLite is an optogenetically controlled Cre system that uses red light in developing zebrafish embryos.
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
CreLite is an optogenetically controlled Cre system that uses red light in developing zebrafish embryos.
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
CreLite is an optogenetically controlled Cre system that uses red light in developing zebrafish embryos.
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
CreLite is an optogenetically controlled Cre system that uses red light in developing zebrafish embryos.
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug phyb-crec
Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity.
Source:
mechanismsupports
CreLite disables Cre by splitting Cre and fusing the inactive halves to the red light-inducible binding partners PhyB and PIF6.
Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6.
Source:
mechanismsupports
Red light at 660 nm in the presence of PCB brings PhyB-CreC and PIF6-CreN together to restore Cre activity.
Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity.
Source:
Comparisons
Source-backed strengths
The underlying CreLite system was shown to restore Cre activity under defined conditions of 660 nm illumination and PCB. Functional validation was reported in transgenic zebrafish embryos using a Cre-dependent multicolor reporter, with recombination detected in multiple tissues including heart, skeletal muscle, and epithelium.
Ranked Citations
- 1.