Source 1primary paper2014ACS Synthetic Biology
Toolkit/phyB/PIF3
phyB/PIF3
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
phyB/PIF3 is a red-light-regulated multi-component optical dimerizer system benchmarked in a yeast transcriptional assay. It uses light-controlled heterodimerization between phyB and PIF3 to regulate transcriptional output.
Usefulness & Problems
Why this is useful
This system is useful as an optogenetic dimerizer for controlling transcription with light in yeast assay formats. The available evidence specifically indicates that it can be compared against other optical dimerizer systems for light sensitivity and fold-activation behavior.
Problem solved
phyB/PIF3 helps address the problem of externally controlling transcriptional activity with a light-responsive protein interaction module. The supplied evidence supports its use in benchmarking red-light-regulated transcriptional control in yeast.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
HeterodimerizationTechniques
Functional AssayTarget processes
transcriptionInput: Light
Implementation Constraints
The evidence places phyB/PIF3 in a yeast transcriptional assay and identifies it as a multi-component optical dimerizer composed of phyB and PIF3. No further construct architecture, chromophore requirement, expression strategy, or illumination parameters are provided in the supplied evidence.
The supplied evidence does not provide quantitative performance values, dynamic range, kinetics, reversibility, or implementation details for phyB/PIF3. Independent replication and validation outside the cited yeast transcriptional benchmark are not established by the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The red-light-regulated systems phyB/PIF3 and phyB/PIF6 showed significant differences in light sensitivity and fold-activation levels in a yeast transcriptional assay.
Using a yeast transcriptional assay, we find significant differences in light sensitivity and fold-activation levels between the red light regulated systems
Approval Evidence
1 source1 linked approval claimfirst-pass slug phyb-pif3
Here, we set about to systematically benchmark the properties of four optical dimerizer systems, CRY2/CIB1, TULIPs, phyB/PIF3, and phyB/PIF6.
Source:
benchmark resultsupports
The red-light-regulated systems phyB/PIF3 and phyB/PIF6 showed significant differences in light sensitivity and fold-activation levels in a yeast transcriptional assay.
Using a yeast transcriptional assay, we find significant differences in light sensitivity and fold-activation levels between the red light regulated systems
Source:
Comparisons
Source-backed strengths
The available literature evidence shows that phyB/PIF3 was included in a systematic benchmark of optical dimerizer systems. In that yeast transcriptional assay, phyB/PIF3 exhibited measurable light sensitivity and fold-activation characteristics that were sufficiently robust for comparison with phyB/PIF6 and other systems.
Ranked Citations
- 1.