Source 1primary paper2015Nucleic Acids Research
Toolkit/PmeR-OPmeR paraben-responsive mammalian transcription-control devices
PmeR-OPmeR paraben-responsive mammalian transcription-control devices
Also known as: inducible and repressible mammalian transcription-control devices, trigger-inducible gene switches
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
PmeR-OPmeR paraben-responsive mammalian transcription-control devices are engineered multi-component gene switches built from the bacterial repressor PmeR and its cognate operator OPmeR. They provide inducible or repressible control of transgene expression in mammalian cells and mice in response to paraben derivatives, including dose-dependent tuning.
Usefulness & Problems
Why this is useful
These devices enable small-molecule control of transcription in mammalian systems using paraben derivatives as triggers. The reported mouse application further indicates utility for noninvasive percutaneous programming of implanted designer cells using commercial paraben-containing skin-care cosmetics.
Source:
we used this feature to percutaneously program transgene expression of subcutaneous designer cell implants using off-the-shelf commercial paraben-containing skin-care cosmetics
Source:
could dose-dependently fine-tune transgene expression in mammalian cells and mice in response to paraben derivatives
Problem solved
The system addresses the need for mammalian gene switches that can externally regulate transgene expression in an inducible or repressible manner. It specifically provides a way to fine-tune transcriptional output in cells and mice using paraben-responsive control elements derived from PmeR and OPmeR.
Source:
we used this feature to percutaneously program transgene expression of subcutaneous designer cell implants using off-the-shelf commercial paraben-containing skin-care cosmetics
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
Computational DesignTarget processes
transcriptionImplementation Constraints
The reported devices are based on the bacterial repressor PmeR and its cognate operator OPmeR, indicating a transcription-factor/operator design strategy for mammalian expression control. Practical use in mice included subcutaneous designer cell implants and topical application of paraben-containing skin-care cosmetics for percutaneous induction, but the supplied evidence does not specify exact construct configurations or delivery vectors.
The supplied evidence is limited to a single 2015 study and does not provide quantitative performance metrics such as dynamic range, leakiness, response time, or paraben specificity. The available text also does not detail construct architecture, safety, long-term stability, or validation across diverse cell types and disease models.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successMouseapplication demo
Inferred from claim c3 during normalization. Topically applied commercial paraben-containing skin-care cosmetics were used to percutaneously program transgene expression of subcutaneous designer cell implants in mice. Derived from claim c3. Quoted text: we used this feature to percutaneously program transgene expression of subcutaneous designer cell implants using off-the-shelf commercial paraben-containing skin-care cosmetics
Source:
successMouseapplication demo
Inferred from claim c3 during normalization. Topically applied commercial paraben-containing skin-care cosmetics were used to percutaneously program transgene expression of subcutaneous designer cell implants in mice. Derived from claim c3. Quoted text: we used this feature to percutaneously program transgene expression of subcutaneous designer cell implants using off-the-shelf commercial paraben-containing skin-care cosmetics
Source:
successMouseapplication demo
Inferred from claim c3 during normalization. Topically applied commercial paraben-containing skin-care cosmetics were used to percutaneously program transgene expression of subcutaneous designer cell implants in mice. Derived from claim c3. Quoted text: we used this feature to percutaneously program transgene expression of subcutaneous designer cell implants using off-the-shelf commercial paraben-containing skin-care cosmetics
Source:
successMouseapplication demo
Inferred from claim c3 during normalization. Topically applied commercial paraben-containing skin-care cosmetics were used to percutaneously program transgene expression of subcutaneous designer cell implants in mice. Derived from claim c3. Quoted text: we used this feature to percutaneously program transgene expression of subcutaneous designer cell implants using off-the-shelf commercial paraben-containing skin-care cosmetics
Source:
successMouseapplication demo
Inferred from claim c3 during normalization. Topically applied commercial paraben-containing skin-care cosmetics were used to percutaneously program transgene expression of subcutaneous designer cell implants in mice. Derived from claim c3. Quoted text: we used this feature to percutaneously program transgene expression of subcutaneous designer cell implants using off-the-shelf commercial paraben-containing skin-care cosmetics
Source:
successMouseapplication demo
Inferred from claim c3 during normalization. Topically applied commercial paraben-containing skin-care cosmetics were used to percutaneously program transgene expression of subcutaneous designer cell implants in mice. Derived from claim c3. Quoted text: we used this feature to percutaneously program transgene expression of subcutaneous designer cell implants using off-the-shelf commercial paraben-containing skin-care cosmetics
Source:
successMouseapplication demo
Inferred from claim c3 during normalization. Topically applied commercial paraben-containing skin-care cosmetics were used to percutaneously program transgene expression of subcutaneous designer cell implants in mice. Derived from claim c3. Quoted text: we used this feature to percutaneously program transgene expression of subcutaneous designer cell implants using off-the-shelf commercial paraben-containing skin-care cosmetics
Source:
Supporting Sources
Ranked Claims
Topically applied commercial paraben-containing skin-care cosmetics were used to percutaneously program transgene expression of subcutaneous designer cell implants in mice.
we used this feature to percutaneously program transgene expression of subcutaneous designer cell implants using off-the-shelf commercial paraben-containing skin-care cosmetics
Topically applied commercial paraben-containing skin-care cosmetics were used to percutaneously program transgene expression of subcutaneous designer cell implants in mice.
we used this feature to percutaneously program transgene expression of subcutaneous designer cell implants using off-the-shelf commercial paraben-containing skin-care cosmetics
Topically applied commercial paraben-containing skin-care cosmetics were used to percutaneously program transgene expression of subcutaneous designer cell implants in mice.
we used this feature to percutaneously program transgene expression of subcutaneous designer cell implants using off-the-shelf commercial paraben-containing skin-care cosmetics
Topically applied commercial paraben-containing skin-care cosmetics were used to percutaneously program transgene expression of subcutaneous designer cell implants in mice.
we used this feature to percutaneously program transgene expression of subcutaneous designer cell implants using off-the-shelf commercial paraben-containing skin-care cosmetics
Topically applied commercial paraben-containing skin-care cosmetics were used to percutaneously program transgene expression of subcutaneous designer cell implants in mice.
we used this feature to percutaneously program transgene expression of subcutaneous designer cell implants using off-the-shelf commercial paraben-containing skin-care cosmetics
Topically applied commercial paraben-containing skin-care cosmetics were used to percutaneously program transgene expression of subcutaneous designer cell implants in mice.
we used this feature to percutaneously program transgene expression of subcutaneous designer cell implants using off-the-shelf commercial paraben-containing skin-care cosmetics
Topically applied commercial paraben-containing skin-care cosmetics were used to percutaneously program transgene expression of subcutaneous designer cell implants in mice.
we used this feature to percutaneously program transgene expression of subcutaneous designer cell implants using off-the-shelf commercial paraben-containing skin-care cosmetics
The authors designed inducible and repressible mammalian transcription-control devices based on PmeR and OPmeR that respond to paraben derivatives.
including the repressor PmeR and its cognate operator OPmeR ... we have designed a set of inducible and repressible mammalian transcription-control devices
The authors designed inducible and repressible mammalian transcription-control devices based on PmeR and OPmeR that respond to paraben derivatives.
including the repressor PmeR and its cognate operator OPmeR ... we have designed a set of inducible and repressible mammalian transcription-control devices
The authors designed inducible and repressible mammalian transcription-control devices based on PmeR and OPmeR that respond to paraben derivatives.
including the repressor PmeR and its cognate operator OPmeR ... we have designed a set of inducible and repressible mammalian transcription-control devices
The authors designed inducible and repressible mammalian transcription-control devices based on PmeR and OPmeR that respond to paraben derivatives.
including the repressor PmeR and its cognate operator OPmeR ... we have designed a set of inducible and repressible mammalian transcription-control devices
The authors designed inducible and repressible mammalian transcription-control devices based on PmeR and OPmeR that respond to paraben derivatives.
including the repressor PmeR and its cognate operator OPmeR ... we have designed a set of inducible and repressible mammalian transcription-control devices
The authors designed inducible and repressible mammalian transcription-control devices based on PmeR and OPmeR that respond to paraben derivatives.
including the repressor PmeR and its cognate operator OPmeR ... we have designed a set of inducible and repressible mammalian transcription-control devices
The authors designed inducible and repressible mammalian transcription-control devices based on PmeR and OPmeR that respond to paraben derivatives.
including the repressor PmeR and its cognate operator OPmeR ... we have designed a set of inducible and repressible mammalian transcription-control devices
The paraben-responsive mammalian transcription-control devices could dose-dependently fine-tune transgene expression in mammalian cells and mice in response to paraben derivatives.
could dose-dependently fine-tune transgene expression in mammalian cells and mice in response to paraben derivatives
The paraben-responsive mammalian transcription-control devices could dose-dependently fine-tune transgene expression in mammalian cells and mice in response to paraben derivatives.
could dose-dependently fine-tune transgene expression in mammalian cells and mice in response to paraben derivatives
The paraben-responsive mammalian transcription-control devices could dose-dependently fine-tune transgene expression in mammalian cells and mice in response to paraben derivatives.
could dose-dependently fine-tune transgene expression in mammalian cells and mice in response to paraben derivatives
The paraben-responsive mammalian transcription-control devices could dose-dependently fine-tune transgene expression in mammalian cells and mice in response to paraben derivatives.
could dose-dependently fine-tune transgene expression in mammalian cells and mice in response to paraben derivatives
The paraben-responsive mammalian transcription-control devices could dose-dependently fine-tune transgene expression in mammalian cells and mice in response to paraben derivatives.
could dose-dependently fine-tune transgene expression in mammalian cells and mice in response to paraben derivatives
The paraben-responsive mammalian transcription-control devices could dose-dependently fine-tune transgene expression in mammalian cells and mice in response to paraben derivatives.
could dose-dependently fine-tune transgene expression in mammalian cells and mice in response to paraben derivatives
The paraben-responsive mammalian transcription-control devices could dose-dependently fine-tune transgene expression in mammalian cells and mice in response to paraben derivatives.
could dose-dependently fine-tune transgene expression in mammalian cells and mice in response to paraben derivatives
The reported control modality is non-invasive, transdermal, and orthogonal for remote control of transgene expression.
The combination of non-invasive, transdermal and orthogonal trigger-inducible remote control of transgene expression
The reported control modality is non-invasive, transdermal, and orthogonal for remote control of transgene expression.
The combination of non-invasive, transdermal and orthogonal trigger-inducible remote control of transgene expression
The reported control modality is non-invasive, transdermal, and orthogonal for remote control of transgene expression.
The combination of non-invasive, transdermal and orthogonal trigger-inducible remote control of transgene expression
The reported control modality is non-invasive, transdermal, and orthogonal for remote control of transgene expression.
The combination of non-invasive, transdermal and orthogonal trigger-inducible remote control of transgene expression
The reported control modality is non-invasive, transdermal, and orthogonal for remote control of transgene expression.
The combination of non-invasive, transdermal and orthogonal trigger-inducible remote control of transgene expression
The reported control modality is non-invasive, transdermal, and orthogonal for remote control of transgene expression.
The combination of non-invasive, transdermal and orthogonal trigger-inducible remote control of transgene expression
The reported control modality is non-invasive, transdermal, and orthogonal for remote control of transgene expression.
The combination of non-invasive, transdermal and orthogonal trigger-inducible remote control of transgene expression
Approval Evidence
1 source4 linked approval claimsfirst-pass slug pmer-opmer-paraben-responsive-mammalian-transcription-control-devices
Capitalizing on genetic componentry, including the repressor PmeR and its cognate operator OPmeR ... we have designed a set of inducible and repressible mammalian transcription-control devices
Source:
application resultsupports
Topically applied commercial paraben-containing skin-care cosmetics were used to percutaneously program transgene expression of subcutaneous designer cell implants in mice.
we used this feature to percutaneously program transgene expression of subcutaneous designer cell implants using off-the-shelf commercial paraben-containing skin-care cosmetics
Source:
engineering resultsupports
The authors designed inducible and repressible mammalian transcription-control devices based on PmeR and OPmeR that respond to paraben derivatives.
including the repressor PmeR and its cognate operator OPmeR ... we have designed a set of inducible and repressible mammalian transcription-control devices
Source:
functional effectsupports
The paraben-responsive mammalian transcription-control devices could dose-dependently fine-tune transgene expression in mammalian cells and mice in response to paraben derivatives.
could dose-dependently fine-tune transgene expression in mammalian cells and mice in response to paraben derivatives
Source:
property assertionsupports
The reported control modality is non-invasive, transdermal, and orthogonal for remote control of transgene expression.
The combination of non-invasive, transdermal and orthogonal trigger-inducible remote control of transgene expression
Source:
Comparisons
Source-backed strengths
The devices were reported to support both inducible and repressible transcription-control formats, increasing design flexibility. They showed dose-dependent fine-tuning of transgene expression in mammalian cells and in mice, and were applied to percutaneous remote control of subcutaneous designer cell implants with commercial paraben-containing cosmetics.
Source:
including the repressor PmeR and its cognate operator OPmeR ... we have designed a set of inducible and repressible mammalian transcription-control devices
Ranked Citations
- 1.