Source 1review2020Journal of Oncology
Toolkit/Potato virus X nanoparticle shuttle
Potato virus X nanoparticle shuttle
Also known as: plant virus-based nanoparticle shuttle, Potato virus X, PVX
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The Potato virus X nanoparticle shuttle is a plant virus-based delivery harness in which photoswitchable protein complexes are displayed on Potato virus X (PVX) particles to enable light-controlled cargo handling. In vitro, PVX particles bearing LOVTRAP, BphP1/QPAS1, or Dronpa145N systems supported reversible loading and unloading of cargo proteins.
Usefulness & Problems
Why this is useful
This system is useful as a light-responsive nanoparticle platform for controlling protein cargo association with and release from PVX surfaces. The cited study states that these PVX-based photoswitchable systems provide a basis for applications in biomedical and biomaterial sciences.
Source:
The new systems provide the basis for promising applications in the biomedical and biomaterial sciences.
Source:
We demonstrated the functionality of all three photo-switchable protein complexes in vitro and the successful loading and unloading of PVX particles.
Source:
Here, we describe a plant virus-based nanoparticle shuttle for the distribution of proteins that can be released when exposed to light.
Problem solved
It addresses the problem of achieving externally controlled, reversible loading and unloading of protein cargo on a nanoparticle scaffold. The evidence specifically supports this function for PVX particles in vitro using photoswitchable protein complexes.
Source:
The new systems provide the basis for promising applications in the biomedical and biomaterial sciences.
Published Workflows
Objective: Engineer a PVX-based nanoparticle shuttle that carries proteins on its surface and releases them upon light exposure by attaching photoswitchable protein systems to PVX particles.
Why it works: The workflow uses light-responsive protein interaction modules on the surface of a PVX carrier so that protein association with the nanoparticle can be controlled by light exposure.
photo-switchable protein complex formation and dissociationlight-triggered release of proteins from PVX particlesattachment of photoswitchable systems to PVX particle surfacesin vitro functional testing of loading and unloading
Stages
- 1.Attach photoswitchable systems to PVX particles(library_build)
This stage creates PVX-based constructs carrying different photoswitchable systems so they can be tested as light-responsive loading and release modules.
Selection: Three different photoswitchable systems were implemented on the PVX surface.
- 2.In vitro functional testing of photoswitchable PVX complexes(confirmatory_validation)
This stage confirms that the PVX-displayed photoswitch systems actually function and support the intended loading and unloading behavior.
Selection: Functionality of the photoswitchable protein complexes and successful loading/unloading of PVX particles in vitro.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A delivery strategy grouped with the mechanism branch because it determines how a system is instantiated and deployed in context.
Mechanisms
light-controlled protein association/dissociationreversible cargo loading and unloading on nanoparticle surfacesTechniques
No technique tags yet.
Target processes
signalingInput: Light
Implementation Constraints
The construct design involves attaching photoswitchable protein complexes to the surface of Potato virus X nanoparticles, consistent with domain fusion and nanoparticle surface display. The evidence names LOVTRAP, BphP1/QPAS1, and Dronpa145N as the implemented systems, but does not provide construct architecture, illumination parameters, expression details, or cofactor requirements.
The available evidence is limited to a single 2022 source and in vitro functionality. No independent replication, in vivo validation, quantitative performance metrics, or application-specific outcomes are provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2022MED
Ranked Claims
These PVX-based photoswitchable systems provide a basis for applications in biomedical and biomaterial sciences.
The new systems provide the basis for promising applications in the biomedical and biomaterial sciences.
These PVX-based photoswitchable systems provide a basis for applications in biomedical and biomaterial sciences.
The new systems provide the basis for promising applications in the biomedical and biomaterial sciences.
These PVX-based photoswitchable systems provide a basis for applications in biomedical and biomaterial sciences.
The new systems provide the basis for promising applications in the biomedical and biomaterial sciences.
These PVX-based photoswitchable systems provide a basis for applications in biomedical and biomaterial sciences.
The new systems provide the basis for promising applications in the biomedical and biomaterial sciences.
These PVX-based photoswitchable systems provide a basis for applications in biomedical and biomaterial sciences.
The new systems provide the basis for promising applications in the biomedical and biomaterial sciences.
These PVX-based photoswitchable systems provide a basis for applications in biomedical and biomaterial sciences.
The new systems provide the basis for promising applications in the biomedical and biomaterial sciences.
These PVX-based photoswitchable systems provide a basis for applications in biomedical and biomaterial sciences.
The new systems provide the basis for promising applications in the biomedical and biomaterial sciences.
These PVX-based photoswitchable systems provide a basis for applications in biomedical and biomaterial sciences.
The new systems provide the basis for promising applications in the biomedical and biomaterial sciences.
All three photoswitchable protein complexes were functional in vitro and supported successful loading and unloading of PVX particles.
We demonstrated the functionality of all three photo-switchable protein complexes in vitro and the successful loading and unloading of PVX particles.
All three photoswitchable protein complexes were functional in vitro and supported successful loading and unloading of PVX particles.
We demonstrated the functionality of all three photo-switchable protein complexes in vitro and the successful loading and unloading of PVX particles.
All three photoswitchable protein complexes were functional in vitro and supported successful loading and unloading of PVX particles.
We demonstrated the functionality of all three photo-switchable protein complexes in vitro and the successful loading and unloading of PVX particles.
All three photoswitchable protein complexes were functional in vitro and supported successful loading and unloading of PVX particles.
We demonstrated the functionality of all three photo-switchable protein complexes in vitro and the successful loading and unloading of PVX particles.
All three photoswitchable protein complexes were functional in vitro and supported successful loading and unloading of PVX particles.
We demonstrated the functionality of all three photo-switchable protein complexes in vitro and the successful loading and unloading of PVX particles.
All three photoswitchable protein complexes were functional in vitro and supported successful loading and unloading of PVX particles.
We demonstrated the functionality of all three photo-switchable protein complexes in vitro and the successful loading and unloading of PVX particles.
All three photoswitchable protein complexes were functional in vitro and supported successful loading and unloading of PVX particles.
We demonstrated the functionality of all three photo-switchable protein complexes in vitro and the successful loading and unloading of PVX particles.
All three photoswitchable protein complexes were functional in vitro and supported successful loading and unloading of PVX particles.
We demonstrated the functionality of all three photo-switchable protein complexes in vitro and the successful loading and unloading of PVX particles.
The study attached LOVTRAP, BphP1/QPAS1, and Dronpa145N to the surface of PVX particles.
In this study, we describe three different systems attached to the surface of PVX particles: LOVTRAP, BphP1/QPAS1 and Dronpa145N.
The study attached LOVTRAP, BphP1/QPAS1, and Dronpa145N to the surface of PVX particles.
In this study, we describe three different systems attached to the surface of PVX particles: LOVTRAP, BphP1/QPAS1 and Dronpa145N.
The study attached LOVTRAP, BphP1/QPAS1, and Dronpa145N to the surface of PVX particles.
In this study, we describe three different systems attached to the surface of PVX particles: LOVTRAP, BphP1/QPAS1 and Dronpa145N.
The study attached LOVTRAP, BphP1/QPAS1, and Dronpa145N to the surface of PVX particles.
In this study, we describe three different systems attached to the surface of PVX particles: LOVTRAP, BphP1/QPAS1 and Dronpa145N.
The study attached LOVTRAP, BphP1/QPAS1, and Dronpa145N to the surface of PVX particles.
In this study, we describe three different systems attached to the surface of PVX particles: LOVTRAP, BphP1/QPAS1 and Dronpa145N.
The study attached LOVTRAP, BphP1/QPAS1, and Dronpa145N to the surface of PVX particles.
In this study, we describe three different systems attached to the surface of PVX particles: LOVTRAP, BphP1/QPAS1 and Dronpa145N.
The study attached LOVTRAP, BphP1/QPAS1, and Dronpa145N to the surface of PVX particles.
In this study, we describe three different systems attached to the surface of PVX particles: LOVTRAP, BphP1/QPAS1 and Dronpa145N.
The study attached LOVTRAP, BphP1/QPAS1, and Dronpa145N to the surface of PVX particles.
In this study, we describe three different systems attached to the surface of PVX particles: LOVTRAP, BphP1/QPAS1 and Dronpa145N.
PVX is used as a presentation system for heterologous proteins and epitopes and has properties favorable for biomedical applications, including good tissue penetration and hydrogel formation that can present signaling molecules and promote cell adhesion.
Potato virus X (PVX) is often used as a presentation system for heterologous proteins and epitopes, and has ideal properties for biomedical applications such as good tissue penetration and the ability to form hydrogels that present signaling molecules and promote cell adhesion.
PVX is used as a presentation system for heterologous proteins and epitopes and has properties favorable for biomedical applications, including good tissue penetration and hydrogel formation that can present signaling molecules and promote cell adhesion.
Potato virus X (PVX) is often used as a presentation system for heterologous proteins and epitopes, and has ideal properties for biomedical applications such as good tissue penetration and the ability to form hydrogels that present signaling molecules and promote cell adhesion.
PVX is used as a presentation system for heterologous proteins and epitopes and has properties favorable for biomedical applications, including good tissue penetration and hydrogel formation that can present signaling molecules and promote cell adhesion.
Potato virus X (PVX) is often used as a presentation system for heterologous proteins and epitopes, and has ideal properties for biomedical applications such as good tissue penetration and the ability to form hydrogels that present signaling molecules and promote cell adhesion.
PVX is used as a presentation system for heterologous proteins and epitopes and has properties favorable for biomedical applications, including good tissue penetration and hydrogel formation that can present signaling molecules and promote cell adhesion.
Potato virus X (PVX) is often used as a presentation system for heterologous proteins and epitopes, and has ideal properties for biomedical applications such as good tissue penetration and the ability to form hydrogels that present signaling molecules and promote cell adhesion.
PVX is used as a presentation system for heterologous proteins and epitopes and has properties favorable for biomedical applications, including good tissue penetration and hydrogel formation that can present signaling molecules and promote cell adhesion.
Potato virus X (PVX) is often used as a presentation system for heterologous proteins and epitopes, and has ideal properties for biomedical applications such as good tissue penetration and the ability to form hydrogels that present signaling molecules and promote cell adhesion.
PVX is used as a presentation system for heterologous proteins and epitopes and has properties favorable for biomedical applications, including good tissue penetration and hydrogel formation that can present signaling molecules and promote cell adhesion.
Potato virus X (PVX) is often used as a presentation system for heterologous proteins and epitopes, and has ideal properties for biomedical applications such as good tissue penetration and the ability to form hydrogels that present signaling molecules and promote cell adhesion.
PVX is used as a presentation system for heterologous proteins and epitopes and has properties favorable for biomedical applications, including good tissue penetration and hydrogel formation that can present signaling molecules and promote cell adhesion.
Potato virus X (PVX) is often used as a presentation system for heterologous proteins and epitopes, and has ideal properties for biomedical applications such as good tissue penetration and the ability to form hydrogels that present signaling molecules and promote cell adhesion.
PVX is used as a presentation system for heterologous proteins and epitopes and has properties favorable for biomedical applications, including good tissue penetration and hydrogel formation that can present signaling molecules and promote cell adhesion.
Potato virus X (PVX) is often used as a presentation system for heterologous proteins and epitopes, and has ideal properties for biomedical applications such as good tissue penetration and the ability to form hydrogels that present signaling molecules and promote cell adhesion.
A PVX-based nanoparticle shuttle can distribute proteins that are released upon light exposure.
Here, we describe a plant virus-based nanoparticle shuttle for the distribution of proteins that can be released when exposed to light.
A PVX-based nanoparticle shuttle can distribute proteins that are released upon light exposure.
Here, we describe a plant virus-based nanoparticle shuttle for the distribution of proteins that can be released when exposed to light.
A PVX-based nanoparticle shuttle can distribute proteins that are released upon light exposure.
Here, we describe a plant virus-based nanoparticle shuttle for the distribution of proteins that can be released when exposed to light.
A PVX-based nanoparticle shuttle can distribute proteins that are released upon light exposure.
Here, we describe a plant virus-based nanoparticle shuttle for the distribution of proteins that can be released when exposed to light.
A PVX-based nanoparticle shuttle can distribute proteins that are released upon light exposure.
Here, we describe a plant virus-based nanoparticle shuttle for the distribution of proteins that can be released when exposed to light.
A PVX-based nanoparticle shuttle can distribute proteins that are released upon light exposure.
Here, we describe a plant virus-based nanoparticle shuttle for the distribution of proteins that can be released when exposed to light.
A PVX-based nanoparticle shuttle can distribute proteins that are released upon light exposure.
Here, we describe a plant virus-based nanoparticle shuttle for the distribution of proteins that can be released when exposed to light.
A PVX-based nanoparticle shuttle can distribute proteins that are released upon light exposure.
Here, we describe a plant virus-based nanoparticle shuttle for the distribution of proteins that can be released when exposed to light.
Approval Evidence
2 sources5 linked approval claimsfirst-pass slugs potato-virus-x, potato-virus-x-nanoparticle-shuttle
Here, we describe a plant virus-based nanoparticle shuttle for the distribution of proteins that can be released when exposed to light. Potato virus X (PVX) is often used as a presentation system for heterologous proteins and epitopes, and has ideal properties for biomedical applications such as good tissue penetration and the ability to form hydrogels that present signaling molecules and promote cell adhesion.
Source:
Related component names explicitly supported by discovered sources include EPR effect, protein corona, personalized protein corona, PEGylation, and Potato virus X.
Source:
application potentialsupports
These PVX-based photoswitchable systems provide a basis for applications in biomedical and biomaterial sciences.
The new systems provide the basis for promising applications in the biomedical and biomaterial sciences.
Source:
functional resultsupports
All three photoswitchable protein complexes were functional in vitro and supported successful loading and unloading of PVX particles.
We demonstrated the functionality of all three photo-switchable protein complexes in vitro and the successful loading and unloading of PVX particles.
Source:
implementationsupports
The study attached LOVTRAP, BphP1/QPAS1, and Dronpa145N to the surface of PVX particles.
In this study, we describe three different systems attached to the surface of PVX particles: LOVTRAP, BphP1/QPAS1 and Dronpa145N.
Source:
platform propertysupports
PVX is used as a presentation system for heterologous proteins and epitopes and has properties favorable for biomedical applications, including good tissue penetration and hydrogel formation that can present signaling molecules and promote cell adhesion.
Potato virus X (PVX) is often used as a presentation system for heterologous proteins and epitopes, and has ideal properties for biomedical applications such as good tissue penetration and the ability to form hydrogels that present signaling molecules and promote cell adhesion.
Source:
tool capabilitysupports
A PVX-based nanoparticle shuttle can distribute proteins that are released upon light exposure.
Here, we describe a plant virus-based nanoparticle shuttle for the distribution of proteins that can be released when exposed to light.
Source:
Comparisons
Source-backed strengths
A key strength is that three distinct photoswitchable systems—LOVTRAP, BphP1/QPAS1, and Dronpa145N—were all reported to be functional on PVX particles in vitro. This indicates that the PVX surface can support multiple light-responsive protein association formats for reversible cargo handling.
Ranked Citations
- 1.
Extracted from this source document.
- 2.