Source 1review2017Annual Review of Biomedical Engineering
Toolkit/programmable genetic circuits
programmable genetic circuits
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Programmable genetic circuits are engineered genetic constructs used to create designer cells with controllable behaviors in mammalian synthetic biology. The cited literature describes circuits that can incorporate targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices to regulate complex cellular functions with high spatial and temporal resolution.
Usefulness & Problems
Why this is useful
These constructs are useful for programming mammalian cell biology with greater precision over when and where cellular functions are executed. The evidence indicates that expanding genetic component availability through improved DNA sequencing and synthesis has supported the construction of such programmable systems.
Source:
The invention of new research tools, including targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices that can recognize and respond to diverse chemical, mechanical, and optical inputs, has enabled precise control of complex cellular behaviors at unprecedented spatial and temporal resolution.
Problem solved
Programmable genetic circuits address the problem of achieving precise control over complex cellular behaviors in engineered mammalian cells. The supplied evidence supports control of cellular functions with high spatial and temporal resolution, but does not provide specific validated examples for recombination or editing outcomes.
Problem links
Need conditional recombination or state switching
DerivedProgrammable genetic circuits are engineered genetic constructs used to create designer cells with controllable behaviors in mammalian synthetic biology. The cited literature describes circuits that can incorporate targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices to regulate complex cellular functions with high spatial and temporal resolution.
Need controllable genome or transcript editing
DerivedProgrammable genetic circuits are engineered genetic constructs used to create designer cells with controllable behaviors in mammalian synthetic biology. The cited literature describes circuits that can incorporate targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices to regulate complex cellular functions with high spatial and temporal resolution.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Target processes
editingrecombinationImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The evidence supports the use of targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices as circuit components in mammalian cells. Improvements in DNA sequencing and synthesis are noted as enabling technologies, but the sources do not specify promoters, vectors, host cell types, or construct design rules.
The provided evidence is broad and field-level rather than tool-specific, so performance metrics, construct architectures, and benchmark comparisons are not available. The supplied text also does not document independent replication, delivery constraints, or direct validation in recombination and editing applications.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices enable precise control of complex cellular behaviors with high spatial and temporal resolution.
The invention of new research tools, including targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices that can recognize and respond to diverse chemical, mechanical, and optical inputs, has enabled precise control of complex cellular behaviors at unprecedented spatial and temporal resolution.
Targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices enable precise control of complex cellular behaviors with high spatial and temporal resolution.
The invention of new research tools, including targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices that can recognize and respond to diverse chemical, mechanical, and optical inputs, has enabled precise control of complex cellular behaviors at unprecedented spatial and temporal resolution.
Targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices enable precise control of complex cellular behaviors with high spatial and temporal resolution.
The invention of new research tools, including targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices that can recognize and respond to diverse chemical, mechanical, and optical inputs, has enabled precise control of complex cellular behaviors at unprecedented spatial and temporal resolution.
Targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices enable precise control of complex cellular behaviors with high spatial and temporal resolution.
The invention of new research tools, including targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices that can recognize and respond to diverse chemical, mechanical, and optical inputs, has enabled precise control of complex cellular behaviors at unprecedented spatial and temporal resolution.
Targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices enable precise control of complex cellular behaviors with high spatial and temporal resolution.
The invention of new research tools, including targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices that can recognize and respond to diverse chemical, mechanical, and optical inputs, has enabled precise control of complex cellular behaviors at unprecedented spatial and temporal resolution.
Targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices enable precise control of complex cellular behaviors with high spatial and temporal resolution.
The invention of new research tools, including targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices that can recognize and respond to diverse chemical, mechanical, and optical inputs, has enabled precise control of complex cellular behaviors at unprecedented spatial and temporal resolution.
Targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices enable precise control of complex cellular behaviors with high spatial and temporal resolution.
The invention of new research tools, including targetable DNA-binding systems such as CRISPR/Cas9 and sensor-actuator devices that can recognize and respond to diverse chemical, mechanical, and optical inputs, has enabled precise control of complex cellular behaviors at unprecedented spatial and temporal resolution.
Improvements in DNA sequencing and synthesis have expanded the set of genetic components available for programming mammalian cell biology.
Continued improvements in the capacity to sequence and synthesize DNA have rapidly increased our understanding of mechanisms of gene function and regulation on a genome-wide scale and have expanded the set of genetic components available for programming cell biology.
Improvements in DNA sequencing and synthesis have expanded the set of genetic components available for programming mammalian cell biology.
Continued improvements in the capacity to sequence and synthesize DNA have rapidly increased our understanding of mechanisms of gene function and regulation on a genome-wide scale and have expanded the set of genetic components available for programming cell biology.
Improvements in DNA sequencing and synthesis have expanded the set of genetic components available for programming mammalian cell biology.
Continued improvements in the capacity to sequence and synthesize DNA have rapidly increased our understanding of mechanisms of gene function and regulation on a genome-wide scale and have expanded the set of genetic components available for programming cell biology.
Improvements in DNA sequencing and synthesis have expanded the set of genetic components available for programming mammalian cell biology.
Continued improvements in the capacity to sequence and synthesize DNA have rapidly increased our understanding of mechanisms of gene function and regulation on a genome-wide scale and have expanded the set of genetic components available for programming cell biology.
Improvements in DNA sequencing and synthesis have expanded the set of genetic components available for programming mammalian cell biology.
Continued improvements in the capacity to sequence and synthesize DNA have rapidly increased our understanding of mechanisms of gene function and regulation on a genome-wide scale and have expanded the set of genetic components available for programming cell biology.
Improvements in DNA sequencing and synthesis have expanded the set of genetic components available for programming mammalian cell biology.
Continued improvements in the capacity to sequence and synthesize DNA have rapidly increased our understanding of mechanisms of gene function and regulation on a genome-wide scale and have expanded the set of genetic components available for programming cell biology.
Improvements in DNA sequencing and synthesis have expanded the set of genetic components available for programming mammalian cell biology.
Continued improvements in the capacity to sequence and synthesize DNA have rapidly increased our understanding of mechanisms of gene function and regulation on a genome-wide scale and have expanded the set of genetic components available for programming cell biology.
These tools were critical for extending synthetic biology techniques from prokaryotic and lower eukaryotic hosts to mammalian systems.
These tools have been critical for the expansion of synthetic biology techniques from prokaryotic and lower eukaryotic hosts to mammalian systems.
These tools were critical for extending synthetic biology techniques from prokaryotic and lower eukaryotic hosts to mammalian systems.
These tools have been critical for the expansion of synthetic biology techniques from prokaryotic and lower eukaryotic hosts to mammalian systems.
These tools were critical for extending synthetic biology techniques from prokaryotic and lower eukaryotic hosts to mammalian systems.
These tools have been critical for the expansion of synthetic biology techniques from prokaryotic and lower eukaryotic hosts to mammalian systems.
These tools were critical for extending synthetic biology techniques from prokaryotic and lower eukaryotic hosts to mammalian systems.
These tools have been critical for the expansion of synthetic biology techniques from prokaryotic and lower eukaryotic hosts to mammalian systems.
These tools were critical for extending synthetic biology techniques from prokaryotic and lower eukaryotic hosts to mammalian systems.
These tools have been critical for the expansion of synthetic biology techniques from prokaryotic and lower eukaryotic hosts to mammalian systems.
These tools were critical for extending synthetic biology techniques from prokaryotic and lower eukaryotic hosts to mammalian systems.
These tools have been critical for the expansion of synthetic biology techniques from prokaryotic and lower eukaryotic hosts to mammalian systems.
These tools were critical for extending synthetic biology techniques from prokaryotic and lower eukaryotic hosts to mammalian systems.
These tools have been critical for the expansion of synthetic biology techniques from prokaryotic and lower eukaryotic hosts to mammalian systems.
Progress in genome editing, epigenome editing, and programmable genetic circuits is expanding approaches to disease prevention, diagnosis, treatment, and personalized theranostic strategies.
Recent progress in the development of genome and epigenome editing tools and in the engineering of designer cells with programmable genetic circuits is expanding approaches to prevent, diagnose, and treat disease and to establish personalized theranostic strategies for next-generation medicines.
Progress in genome editing, epigenome editing, and programmable genetic circuits is expanding approaches to disease prevention, diagnosis, treatment, and personalized theranostic strategies.
Recent progress in the development of genome and epigenome editing tools and in the engineering of designer cells with programmable genetic circuits is expanding approaches to prevent, diagnose, and treat disease and to establish personalized theranostic strategies for next-generation medicines.
Progress in genome editing, epigenome editing, and programmable genetic circuits is expanding approaches to disease prevention, diagnosis, treatment, and personalized theranostic strategies.
Recent progress in the development of genome and epigenome editing tools and in the engineering of designer cells with programmable genetic circuits is expanding approaches to prevent, diagnose, and treat disease and to establish personalized theranostic strategies for next-generation medicines.
Progress in genome editing, epigenome editing, and programmable genetic circuits is expanding approaches to disease prevention, diagnosis, treatment, and personalized theranostic strategies.
Recent progress in the development of genome and epigenome editing tools and in the engineering of designer cells with programmable genetic circuits is expanding approaches to prevent, diagnose, and treat disease and to establish personalized theranostic strategies for next-generation medicines.
Progress in genome editing, epigenome editing, and programmable genetic circuits is expanding approaches to disease prevention, diagnosis, treatment, and personalized theranostic strategies.
Recent progress in the development of genome and epigenome editing tools and in the engineering of designer cells with programmable genetic circuits is expanding approaches to prevent, diagnose, and treat disease and to establish personalized theranostic strategies for next-generation medicines.
Progress in genome editing, epigenome editing, and programmable genetic circuits is expanding approaches to disease prevention, diagnosis, treatment, and personalized theranostic strategies.
Recent progress in the development of genome and epigenome editing tools and in the engineering of designer cells with programmable genetic circuits is expanding approaches to prevent, diagnose, and treat disease and to establish personalized theranostic strategies for next-generation medicines.
Progress in genome editing, epigenome editing, and programmable genetic circuits is expanding approaches to disease prevention, diagnosis, treatment, and personalized theranostic strategies.
Recent progress in the development of genome and epigenome editing tools and in the engineering of designer cells with programmable genetic circuits is expanding approaches to prevent, diagnose, and treat disease and to establish personalized theranostic strategies for next-generation medicines.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug programmable-genetic-circuits
the engineering of designer cells with programmable genetic circuits
Source:
field enabling technology summarysupports
Improvements in DNA sequencing and synthesis have expanded the set of genetic components available for programming mammalian cell biology.
Continued improvements in the capacity to sequence and synthesize DNA have rapidly increased our understanding of mechanisms of gene function and regulation on a genome-wide scale and have expanded the set of genetic components available for programming cell biology.
Source:
field expansion summarysupports
These tools were critical for extending synthetic biology techniques from prokaryotic and lower eukaryotic hosts to mammalian systems.
These tools have been critical for the expansion of synthetic biology techniques from prokaryotic and lower eukaryotic hosts to mammalian systems.
Source:
translational potential summarysupports
Progress in genome editing, epigenome editing, and programmable genetic circuits is expanding approaches to disease prevention, diagnosis, treatment, and personalized theranostic strategies.
Recent progress in the development of genome and epigenome editing tools and in the engineering of designer cells with programmable genetic circuits is expanding approaches to prevent, diagnose, and treat disease and to establish personalized theranostic strategies for next-generation medicines.
Source:
Comparisons
Source-backed strengths
A key reported strength is the ability to combine targetable DNA-binding systems and sensor-actuator devices for precise regulation of complex cellular behaviors. The literature specifically attributes high spatial and temporal resolution to these approaches in mammalian synthetic biology.
Compared with intron-containing CRISPRa construct
programmable genetic circuits and intron-containing CRISPRa construct address a similar problem space because they share editing, recombination.
Shared frame: same top-level item type; shared target processes: editing, recombination
Compared with microfluidic organ-on-chip platforms
programmable genetic circuits and microfluidic organ-on-chip platforms address a similar problem space because they share editing, recombination.
Shared frame: same top-level item type; shared target processes: editing, recombination
Strengths here: looks easier to implement in practice.
Compared with PMNT mixed with single-stranded DNA color reporter
programmable genetic circuits and PMNT mixed with single-stranded DNA color reporter address a similar problem space because they share editing, recombination.
Shared frame: same top-level item type; shared target processes: editing, recombination
Ranked Citations
- 1.