Source 1primary paper2025MED
Toolkit/ProKAS
ProKAS
Also known as: Proteomic Kinase Activity Sensor, proteomic kinase activity sensor technique
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Here we develop a proteomic kinase activity sensor technique (ProKAS) for the analysis of kinase signaling using mass spectrometry.
Usefulness & Problems
Why this is useful
ProKAS is a proteomic kinase activity sensor technique for analyzing kinase signaling by mass spectrometry. It uses peptide sensors with amino acid barcodes to enable multiplexed and spatial measurements in cells.; analysis of kinase signaling using mass spectrometry; quantitative multiplexed monitoring of kinase activity; spatial monitoring of kinase signaling in cells; kinetic and screening applications
Source:
ProKAS is a proteomic kinase activity sensor technique for analyzing kinase signaling by mass spectrometry. It uses peptide sensors with amino acid barcodes to enable multiplexed and spatial measurements in cells.
Source:
analysis of kinase signaling using mass spectrometry
Source:
quantitative multiplexed monitoring of kinase activity
Source:
spatial monitoring of kinase signaling in cells
Source:
kinetic and screening applications
Problem solved
It addresses the need for precise quantitative and spatial measurements of kinase activity in vivo. The platform is presented as a way to deconvolute complex signaling networks and assess drug or genetic perturbation effects.; provides quantitative measurements of kinase activity in vivo; captures spatial information about kinase activity
Source:
It addresses the need for precise quantitative and spatial measurements of kinase activity in vivo. The platform is presented as a way to deconvolute complex signaling networks and assess drug or genetic perturbation effects.
Source:
provides quantitative measurements of kinase activity in vivo
Source:
captures spatial information about kinase activity
Problem links
captures spatial information about kinase activity
LiteratureIt addresses the need for precise quantitative and spatial measurements of kinase activity in vivo. The platform is presented as a way to deconvolute complex signaling networks and assess drug or genetic perturbation effects.
Source:
It addresses the need for precise quantitative and spatial measurements of kinase activity in vivo. The platform is presented as a way to deconvolute complex signaling networks and assess drug or genetic perturbation effects.
provides quantitative measurements of kinase activity in vivo
LiteratureIt addresses the need for precise quantitative and spatial measurements of kinase activity in vivo. The platform is presented as a way to deconvolute complex signaling networks and assess drug or genetic perturbation effects.
Source:
It addresses the need for precise quantitative and spatial measurements of kinase activity in vivo. The platform is presented as a way to deconvolute complex signaling networks and assess drug or genetic perturbation effects.
Published Workflows
Objective: Develop a mass-spectrometry-based proteomic sensor system for quantitative, multiplexed, and spatial monitoring of kinase signaling in cells, including DNA damage response kinases and expandable design to other kinases.
Why it works: The workflow combines multiplexed peptide sensors with amino acid barcodes and mass spectrometry so multiple kinase-responsive substrates can be measured together, including across subcellular contexts. The in silico design component is intended to generate specific substrate peptides for expansion to other kinases.
monitoring kinase activity through substrate peptide phosphorylation readoutsresolving compartment-specific kinase signaling differencesmass spectrometrytandem peptide sensor arraysamino acid barcodingin silico substrate peptide design
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
mass spectrometric barcode-based multiplexed detectionpeptide sensor phosphorylation readoutsubcellularly resolved kinase activity measurementTarget processes
recombinationselectionsignalingInput: Chemical
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The abstract states that ProKAS uses mass spectrometry and a tandem array of peptide sensors with amino acid barcodes. Cellular deployment is implied because the system is used to probe kinase action in cells.; uses mass spectrometry; is based on a tandem array of peptide sensors with amino acid barcodes
Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successMammalian Cell Lineapplication demo
mass spectrometry
Inferred from claim cl3 during normalization. An engineered ProKAS module simultaneously monitored ATR, ATM, and CHK1 activities in response to genotoxic drugs and revealed differences across nucleus, cytosol, and replication factories. Derived from claim cl3. Quoted text: We engineered a ProKAS module to simultaneously monitor the activities of the DNA damage response kinases ATR, ATM, and CHK1 in response to genotoxic drugs, while also uncovering differences between these signaling responses in the nucleus, cytosol, and replication factories.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim cl5 during normalization. ProKAS is a versatile system for systematic and spatial probing of kinase action in cells. Derived from claim cl5. Quoted text: Overall, ProKAS is a versatile system for systematically and spatially probing kinase action in cells.
Source:
Supporting Sources
Ranked Claims
An engineered ProKAS module simultaneously monitored ATR, ATM, and CHK1 activities in response to genotoxic drugs and revealed differences across nucleus, cytosol, and replication factories.
We engineered a ProKAS module to simultaneously monitor the activities of the DNA damage response kinases ATR, ATM, and CHK1 in response to genotoxic drugs, while also uncovering differences between these signaling responses in the nucleus, cytosol, and replication factories.
ProKAS uses a tandem array of peptide sensors with amino acid barcodes to enable multiplexed spatial, kinetic, and screening analyses.
ProKAS is based on a tandem array of peptide sensors with amino acid barcodes that allow multiplexed analysis for spatial, kinetic, and screening applications.
ProKAS is a versatile system for systematic and spatial probing of kinase action in cells.
Overall, ProKAS is a versatile system for systematically and spatially probing kinase action in cells.
ProKAS is a proteomic kinase activity sensor technique for analysis of kinase signaling using mass spectrometry.
Here we develop a proteomic kinase activity sensor technique (ProKAS) for the analysis of kinase signaling using mass spectrometry.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug prokas
Here we develop a proteomic kinase activity sensor technique (ProKAS) for the analysis of kinase signaling using mass spectrometry.
Source:
application scopesupports
An engineered ProKAS module simultaneously monitored ATR, ATM, and CHK1 activities in response to genotoxic drugs and revealed differences across nucleus, cytosol, and replication factories.
We engineered a ProKAS module to simultaneously monitor the activities of the DNA damage response kinases ATR, ATM, and CHK1 in response to genotoxic drugs, while also uncovering differences between these signaling responses in the nucleus, cytosol, and replication factories.
Source:
method capabilitysupports
ProKAS uses a tandem array of peptide sensors with amino acid barcodes to enable multiplexed spatial, kinetic, and screening analyses.
ProKAS is based on a tandem array of peptide sensors with amino acid barcodes that allow multiplexed analysis for spatial, kinetic, and screening applications.
Source:
overall utilitysupports
ProKAS is a versatile system for systematic and spatial probing of kinase action in cells.
Overall, ProKAS is a versatile system for systematically and spatially probing kinase action in cells.
Source:
tool developmentsupports
ProKAS is a proteomic kinase activity sensor technique for analysis of kinase signaling using mass spectrometry.
Here we develop a proteomic kinase activity sensor technique (ProKAS) for the analysis of kinase signaling using mass spectrometry.
Source:
Comparisons
Source-stated alternatives
No direct alternative platform is named in the abstract. The paper contrasts ProKAS with the general unmet need for precise in vivo and spatial kinase activity measurements.
Source:
No direct alternative platform is named in the abstract. The paper contrasts ProKAS with the general unmet need for precise in vivo and spatial kinase activity measurements.
Source-backed strengths
supports multiplexed analysis via amino acid barcodes; supports spatial, kinetic, and screening applications; described as expandable to other kinases
Source:
supports multiplexed analysis via amino acid barcodes
Source:
supports spatial, kinetic, and screening applications
Source:
described as expandable to other kinases
Compared with spatial atlases
No direct alternative platform is named in the abstract. The paper contrasts ProKAS with the general unmet need for precise in vivo and spatial kinase activity measurements.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports multiplexed analysis via amino acid barcodes; supports spatial, kinetic, and screening applications; described as expandable to other kinases.
Source:
No direct alternative platform is named in the abstract. The paper contrasts ProKAS with the general unmet need for precise in vivo and spatial kinase activity measurements.
Ranked Citations
- 1.