Source 1review2022Frontiers in Bioengineering and Biotechnology
Toolkit/protein engineering approaches for opto-protein development
protein engineering approaches for opto-protein development
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Protein engineering approaches for opto-protein development are review-described strategies for creating and optimizing non-neuronal light-regulatable proteins. The approaches specifically include modifying photosensitive domains and fusing them to effector domains to generate light-controllable functions.
Usefulness & Problems
Why this is useful
These approaches are useful for developing novel optogenetic proteins whose activity can be regulated by light. The cited review presents them as protein engineering and synthetic biology strategies that may aid opto-protein development and optimization.
Problem solved
This methodology addresses the challenge of engineering proteins with light-controllable functions in non-neuronal biological contexts. The evidence supports that it does so by leveraging photosensitive domains and coupling them to effector domains.
Problem links
Need precise spatiotemporal control with light input
DerivedProtein engineering approaches for opto-protein development comprise a review-defined methodology for creating and optimizing non-neuronal light-regulatable proteins. The described strategies include modification of photosensitive domains and fusion of these domains to effector domains to generate light-controllable functions.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Mechanisms
light-dependent control mediated by photosensitive domainslight-dependent control mediated by photosensitive domainsTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: builder
The available evidence indicates construct-level design centered on photosensitive domains and their fusion to effector domains. No specific cofactors, host organisms, expression systems, linker designs, or delivery methods are provided in the supplied evidence.
The supplied evidence is review-level and does not provide specific proteins, photoreceptors, wavelengths, quantitative performance, or experimental validation outcomes. Independent replication, comparative benchmarking, and implementation constraints are not described in the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
Modification of photosensitive domains and their fusion to effector domains are discussed as general strategies for creating light-controllable functions.
general strategies for creating light-controllable functions, modification of the photosensitive domains and their fusion to effector domains
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
Approval Evidence
1 source1 linked approval claimfirst-pass slug protein-engineering-approaches-for-opto-protein-development
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins).
Source:
review scopesupports
The review focuses on engineering and optimization of non-neuronal light-regulatable proteins using protein engineering and synthetic biology approaches.
This review highlights different protein engineering and synthetic biology approaches, which might aid in the development and optimization of novel optogenetic proteins (Opto-proteins). Focusing on non-neuronal optogenetics
Source:
Comparisons
Source-backed strengths
A key strength is conceptual generality: the review identifies both photosensitive-domain modification and photosensitive-domain/effector-domain fusion as reusable design strategies. The evidence also supports applicability to both development and optimization of novel optogenetic proteins.
Compared with doxycycline-dependent photoactivated gene expression
protein engineering approaches for opto-protein development and doxycycline-dependent photoactivated gene expression address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with oligomerization reactions
protein engineering approaches for opto-protein development and oligomerization reactions address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with targeted mutagenesis of Arabidopsis phototropins
protein engineering approaches for opto-protein development and targeted mutagenesis of Arabidopsis phototropins address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Ranked Citations
- 1.