Source 1primary paper2019Proceedings of the National Academy of Sciences
Toolkit/targeted mutagenesis of Arabidopsis phototropins
targeted mutagenesis of Arabidopsis phototropins
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Targeted mutagenesis of Arabidopsis phototropins is an engineering method that tunes phototropin photocycle lifetime to shorten or prolong light-activated receptor signaling. In vitro, mutagenesis altered photocycle lifetime, and in planta slow-photocycling phot1 or phot2 variants extended activation and increased biomass under low-light conditions.
Usefulness & Problems
Why this is useful
This method is useful for adjusting the duration of blue-light photoreceptor activation in plants without changing the input modality. The reported slow-photocycling phot1 or phot2 variants improved light sensitivity and increased biomass production under low-light conditions.
Problem solved
It addresses the problem of limited control over how long Arabidopsis phototropins remain active after light stimulation. The method enables engineered reduction or extension of photoreceptor activation duration, with demonstrated benefit for low-light growth performance.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Techniques
No technique tags yet.
Target processes
recombinationInput: Light
Implementation Constraints
Implementation involves targeted mutagenesis of Arabidopsis phototropins, specifically phot1 or phot2, to alter photocycle lifetime. The evidence supports in vitro characterization of photocycle changes and in planta testing of engineered variants, but does not provide construct design, expression strategy, or cofactor requirements.
The supplied evidence is limited to Arabidopsis phototropins and a single 2019 study, so generality across species, photoreceptors, or growth conditions is not established. Practical performance details such as mutation identities, effect sizes, spectral properties, and trade-offs outside low-light biomass production are not provided in the evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Engineered phototropin variants can reduce or extend the duration of photoreceptor activation in planta.
these variants can be used to reduce or extend the duration of photoreceptor activation in planta
Engineered phototropin variants can reduce or extend the duration of photoreceptor activation in planta.
these variants can be used to reduce or extend the duration of photoreceptor activation in planta
Engineered phototropin variants can reduce or extend the duration of photoreceptor activation in planta.
these variants can be used to reduce or extend the duration of photoreceptor activation in planta
Engineered phototropin variants can reduce or extend the duration of photoreceptor activation in planta.
these variants can be used to reduce or extend the duration of photoreceptor activation in planta
Engineered phototropin variants can reduce or extend the duration of photoreceptor activation in planta.
these variants can be used to reduce or extend the duration of photoreceptor activation in planta
Engineered phototropin variants can reduce or extend the duration of photoreceptor activation in planta.
these variants can be used to reduce or extend the duration of photoreceptor activation in planta
Engineered phototropin variants can reduce or extend the duration of photoreceptor activation in planta.
these variants can be used to reduce or extend the duration of photoreceptor activation in planta
Plants engineered to have a slow-photocycling variant of phot1 or phot2 displayed increased biomass production under low-light conditions as a consequence of improved sensitivity.
plants engineered to have a slow-photocycling variant of phot1 or phot2 displayed increased biomass production under low-light conditions as a consequence of their improved sensitivity
Plants engineered to have a slow-photocycling variant of phot1 or phot2 displayed increased biomass production under low-light conditions as a consequence of improved sensitivity.
plants engineered to have a slow-photocycling variant of phot1 or phot2 displayed increased biomass production under low-light conditions as a consequence of their improved sensitivity
Plants engineered to have a slow-photocycling variant of phot1 or phot2 displayed increased biomass production under low-light conditions as a consequence of improved sensitivity.
plants engineered to have a slow-photocycling variant of phot1 or phot2 displayed increased biomass production under low-light conditions as a consequence of their improved sensitivity
Plants engineered to have a slow-photocycling variant of phot1 or phot2 displayed increased biomass production under low-light conditions as a consequence of improved sensitivity.
plants engineered to have a slow-photocycling variant of phot1 or phot2 displayed increased biomass production under low-light conditions as a consequence of their improved sensitivity
Plants engineered to have a slow-photocycling variant of phot1 or phot2 displayed increased biomass production under low-light conditions as a consequence of improved sensitivity.
plants engineered to have a slow-photocycling variant of phot1 or phot2 displayed increased biomass production under low-light conditions as a consequence of their improved sensitivity
Plants engineered to have a slow-photocycling variant of phot1 or phot2 displayed increased biomass production under low-light conditions as a consequence of improved sensitivity.
plants engineered to have a slow-photocycling variant of phot1 or phot2 displayed increased biomass production under low-light conditions as a consequence of their improved sensitivity
Plants engineered to have a slow-photocycling variant of phot1 or phot2 displayed increased biomass production under low-light conditions as a consequence of improved sensitivity.
plants engineered to have a slow-photocycling variant of phot1 or phot2 displayed increased biomass production under low-light conditions as a consequence of their improved sensitivity
Targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro.
We demonstrate that targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro
Targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro.
We demonstrate that targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro
Targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro.
We demonstrate that targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro
Targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro.
We demonstrate that targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro
Targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro.
We demonstrate that targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro
Targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro.
We demonstrate that targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro
Targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro.
We demonstrate that targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro
Engineering photoreceptors is feasible for manipulating plant growth and may enhance photosynthetic competence under suboptimal light regimes.
these findings demonstrate the feasibility of engineering photoreceptors to manipulate plant growth and offer additional opportunities to enhance photosynthetic competence, particularly under suboptimal light regimes
Engineering photoreceptors is feasible for manipulating plant growth and may enhance photosynthetic competence under suboptimal light regimes.
these findings demonstrate the feasibility of engineering photoreceptors to manipulate plant growth and offer additional opportunities to enhance photosynthetic competence, particularly under suboptimal light regimes
Engineering photoreceptors is feasible for manipulating plant growth and may enhance photosynthetic competence under suboptimal light regimes.
these findings demonstrate the feasibility of engineering photoreceptors to manipulate plant growth and offer additional opportunities to enhance photosynthetic competence, particularly under suboptimal light regimes
Engineering photoreceptors is feasible for manipulating plant growth and may enhance photosynthetic competence under suboptimal light regimes.
these findings demonstrate the feasibility of engineering photoreceptors to manipulate plant growth and offer additional opportunities to enhance photosynthetic competence, particularly under suboptimal light regimes
Engineering photoreceptors is feasible for manipulating plant growth and may enhance photosynthetic competence under suboptimal light regimes.
these findings demonstrate the feasibility of engineering photoreceptors to manipulate plant growth and offer additional opportunities to enhance photosynthetic competence, particularly under suboptimal light regimes
Engineering photoreceptors is feasible for manipulating plant growth and may enhance photosynthetic competence under suboptimal light regimes.
these findings demonstrate the feasibility of engineering photoreceptors to manipulate plant growth and offer additional opportunities to enhance photosynthetic competence, particularly under suboptimal light regimes
Engineering photoreceptors is feasible for manipulating plant growth and may enhance photosynthetic competence under suboptimal light regimes.
these findings demonstrate the feasibility of engineering photoreceptors to manipulate plant growth and offer additional opportunities to enhance photosynthetic competence, particularly under suboptimal light regimes
Slowing the phototropin photocycle enhanced several light-capturing responses.
slowing the phototropin photocycle enhanced several light-capturing responses
Accelerating the phototropin photocycle reduced phototropin sensitivity for chloroplast accumulation movement.
accelerating it reduced phototropin's sensitivity for chloroplast accumulation movement
Approval Evidence
1 source4 linked approval claimsfirst-pass slug targeted-mutagenesis-of-arabidopsis-phototropins
We demonstrate that targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro.
Source:
activity duration controlsupports
Engineered phototropin variants can reduce or extend the duration of photoreceptor activation in planta.
these variants can be used to reduce or extend the duration of photoreceptor activation in planta
Source:
biomass effectsupports
Plants engineered to have a slow-photocycling variant of phot1 or phot2 displayed increased biomass production under low-light conditions as a consequence of improved sensitivity.
plants engineered to have a slow-photocycling variant of phot1 or phot2 displayed increased biomass production under low-light conditions as a consequence of their improved sensitivity
Source:
engineering effectsupports
Targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro.
We demonstrate that targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro
Source:
feasibilitysupports
Engineering photoreceptors is feasible for manipulating plant growth and may enhance photosynthetic competence under suboptimal light regimes.
these findings demonstrate the feasibility of engineering photoreceptors to manipulate plant growth and offer additional opportunities to enhance photosynthetic competence, particularly under suboptimal light regimes
Source:
Comparisons
Source-backed strengths
The approach was shown to both decrease and increase Arabidopsis phototropin photocycle lifetime in vitro, indicating bidirectional tuning of receptor kinetics. In planta, engineered slow-photocycling variants of phot1 or phot2 extended activation and were associated with increased biomass production under low-light conditions.
Source:
We demonstrate that targeted mutagenesis can decrease or increase the photocycle lifetime of Arabidopsis phototropins in vitro
Ranked Citations
- 1.