Source 1primary paper2025MED
Toolkit/PS Intein
PS Intein
Also known as: photoswitchable intein
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
PS Intein is a photoswitchable intein engineered by allosterically modulating the small autocatalytic gp41-1 intein with a tandem Vivid photoreceptor. It enables light control of covalent protein binding and cleavage in cells.
Usefulness & Problems
Why this is useful
PS Intein provides optical control over intein-mediated covalent protein ligation and cleavage, enabling conditional regulation of protein function in cellular contexts. The cited study further indicates that its high responsiveness and multi-input integration capability support intersectional cell targeting and regulation of gene expression and cell fate.
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PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
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The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
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PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
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We introduce a photoswitchable intein (PS Intein) engineered by allosterically modulating a small autocatalytic gp41-1 intein with tandem Vivid photoreceptor.
Problem solved
PS Intein addresses the need for a genetically encoded system that couples light input to covalent protein binding and cleavage rather than only reversible association. The available evidence also indicates that it helps implement intersectional control schemes using cancer- and tumor-microenvironment-specific promoters.
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PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
Source:
The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
Published Workflows
Objective: Engineer a light-responsive intein platform for precise control of covalent protein binding, cleavage, and release in mammalian cells.
Why it works: The abstract states that PS Intein was engineered by allosterically modulating gp41-1 intein with tandem Vivid photoreceptor, linking light sensing to intein-mediated covalent protein reactions.
allosteric modulation of an autocatalytic intein by a photoreceptorlight-induced covalent protein bindinglight-induced protein cleavagelight-induced protein releaseprotein engineeringoptogenetic control
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
PS Intein is described as an engineered fusion in which a tandem Vivid photoreceptor allosterically modulates the gp41-1 intein, so construct architecture is central to function. The available evidence establishes light as the input modality and cellular operation, but does not specify wavelength, cofactor requirements, expression system, or delivery method.
The provided evidence is limited to a single 2025 source and brief application-level claims. Quantitative performance metrics, illumination parameters, dynamic range, kinetics, off-state leak, and breadth of validation across organisms or tissues are not provided here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successMammalian Cell Lineapplication demo
Inferred from claim cl3 during normalization. PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins. Derived from claim cl3. Quoted text: PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
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successMammalian Cell Lineapplication demo
Inferred from claim cl5 during normalization. The high responsiveness and multi-input integration capability of PS Intein allow intersectional cell targeting using cancer- and tumor microenvironment-specific promoters. Derived from claim cl5. Quoted text: The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
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successMammalian Cell Lineapplication demo
Inferred from claim cl3 during normalization. PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins. Derived from claim cl3. Quoted text: PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
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successMammalian Cell Lineapplication demo
Inferred from claim cl5 during normalization. The high responsiveness and multi-input integration capability of PS Intein allow intersectional cell targeting using cancer- and tumor microenvironment-specific promoters. Derived from claim cl5. Quoted text: The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
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successMammalian Cell Lineapplication demo
Inferred from claim cl3 during normalization. PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins. Derived from claim cl3. Quoted text: PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
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successMammalian Cell Lineapplication demo
Inferred from claim cl5 during normalization. The high responsiveness and multi-input integration capability of PS Intein allow intersectional cell targeting using cancer- and tumor microenvironment-specific promoters. Derived from claim cl5. Quoted text: The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
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successMammalian Cell Lineapplication demo
Inferred from claim cl3 during normalization. PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins. Derived from claim cl3. Quoted text: PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
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successMammalian Cell Lineapplication demo
Inferred from claim cl5 during normalization. The high responsiveness and multi-input integration capability of PS Intein allow intersectional cell targeting using cancer- and tumor microenvironment-specific promoters. Derived from claim cl5. Quoted text: The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
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successMammalian Cell Lineapplication demo
Inferred from claim cl3 during normalization. PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins. Derived from claim cl3. Quoted text: PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
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successMammalian Cell Lineapplication demo
Inferred from claim cl5 during normalization. The high responsiveness and multi-input integration capability of PS Intein allow intersectional cell targeting using cancer- and tumor microenvironment-specific promoters. Derived from claim cl5. Quoted text: The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim cl3 during normalization. PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins. Derived from claim cl3. Quoted text: PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim cl5 during normalization. The high responsiveness and multi-input integration capability of PS Intein allow intersectional cell targeting using cancer- and tumor microenvironment-specific promoters. Derived from claim cl5. Quoted text: The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim cl3 during normalization. PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins. Derived from claim cl3. Quoted text: PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim cl5 during normalization. The high responsiveness and multi-input integration capability of PS Intein allow intersectional cell targeting using cancer- and tumor microenvironment-specific promoters. Derived from claim cl5. Quoted text: The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim cl3 during normalization. PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins. Derived from claim cl3. Quoted text: PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim cl5 during normalization. The high responsiveness and multi-input integration capability of PS Intein allow intersectional cell targeting using cancer- and tumor microenvironment-specific promoters. Derived from claim cl5. Quoted text: The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
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Supporting Sources
Ranked Claims
PS Intein-based systems can be used to regulate gene expression and cell fate.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
PS Intein-based systems can be used to regulate gene expression and cell fate.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
PS Intein-based systems can be used to regulate gene expression and cell fate.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
PS Intein-based systems can be used to regulate gene expression and cell fate.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
PS Intein-based systems can be used to regulate gene expression and cell fate.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
PS Intein-based systems can be used to regulate gene expression and cell fate.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
PS Intein-based systems can be used to regulate gene expression and cell fate.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
PS Intein-based systems can be used to regulate gene expression and cell fate.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
The high responsiveness and multi-input integration capability of PS Intein allow intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
The high responsiveness and multi-input integration capability of PS Intein allow intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
The high responsiveness and multi-input integration capability of PS Intein allow intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
The high responsiveness and multi-input integration capability of PS Intein allow intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
The high responsiveness and multi-input integration capability of PS Intein allow intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
The high responsiveness and multi-input integration capability of PS Intein allow intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
The high responsiveness and multi-input integration capability of PS Intein allow intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
The high responsiveness and multi-input integration capability of PS Intein allow intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
PS Intein tolerates various fusions and insertions, facilitating application in diverse cellular contexts.
PS Intein tolerates various fusions and insertions, facilitating its application in diverse cellular contexts.
PS Intein tolerates various fusions and insertions, facilitating application in diverse cellular contexts.
PS Intein tolerates various fusions and insertions, facilitating its application in diverse cellular contexts.
PS Intein tolerates various fusions and insertions, facilitating application in diverse cellular contexts.
PS Intein tolerates various fusions and insertions, facilitating its application in diverse cellular contexts.
PS Intein tolerates various fusions and insertions, facilitating application in diverse cellular contexts.
PS Intein tolerates various fusions and insertions, facilitating its application in diverse cellular contexts.
PS Intein tolerates various fusions and insertions, facilitating application in diverse cellular contexts.
PS Intein tolerates various fusions and insertions, facilitating its application in diverse cellular contexts.
PS Intein tolerates various fusions and insertions, facilitating application in diverse cellular contexts.
PS Intein tolerates various fusions and insertions, facilitating its application in diverse cellular contexts.
PS Intein tolerates various fusions and insertions, facilitating application in diverse cellular contexts.
PS Intein tolerates various fusions and insertions, facilitating its application in diverse cellular contexts.
PS Intein tolerates various fusions and insertions, facilitating application in diverse cellular contexts.
PS Intein tolerates various fusions and insertions, facilitating its application in diverse cellular contexts.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
This paper introduces PS Intein, a photoswitchable intein engineered by allosterically modulating gp41-1 intein with tandem Vivid photoreceptor.
We introduce a photoswitchable intein (PS Intein) engineered by allosterically modulating a small autocatalytic gp41-1 intein with tandem Vivid photoreceptor.
This paper introduces PS Intein, a photoswitchable intein engineered by allosterically modulating gp41-1 intein with tandem Vivid photoreceptor.
We introduce a photoswitchable intein (PS Intein) engineered by allosterically modulating a small autocatalytic gp41-1 intein with tandem Vivid photoreceptor.
This paper introduces PS Intein, a photoswitchable intein engineered by allosterically modulating gp41-1 intein with tandem Vivid photoreceptor.
We introduce a photoswitchable intein (PS Intein) engineered by allosterically modulating a small autocatalytic gp41-1 intein with tandem Vivid photoreceptor.
This paper introduces PS Intein, a photoswitchable intein engineered by allosterically modulating gp41-1 intein with tandem Vivid photoreceptor.
We introduce a photoswitchable intein (PS Intein) engineered by allosterically modulating a small autocatalytic gp41-1 intein with tandem Vivid photoreceptor.
This paper introduces PS Intein, a photoswitchable intein engineered by allosterically modulating gp41-1 intein with tandem Vivid photoreceptor.
We introduce a photoswitchable intein (PS Intein) engineered by allosterically modulating a small autocatalytic gp41-1 intein with tandem Vivid photoreceptor.
This paper introduces PS Intein, a photoswitchable intein engineered by allosterically modulating gp41-1 intein with tandem Vivid photoreceptor.
We introduce a photoswitchable intein (PS Intein) engineered by allosterically modulating a small autocatalytic gp41-1 intein with tandem Vivid photoreceptor.
This paper introduces PS Intein, a photoswitchable intein engineered by allosterically modulating gp41-1 intein with tandem Vivid photoreceptor.
We introduce a photoswitchable intein (PS Intein) engineered by allosterically modulating a small autocatalytic gp41-1 intein with tandem Vivid photoreceptor.
This paper introduces PS Intein, a photoswitchable intein engineered by allosterically modulating gp41-1 intein with tandem Vivid photoreceptor.
We introduce a photoswitchable intein (PS Intein) engineered by allosterically modulating a small autocatalytic gp41-1 intein with tandem Vivid photoreceptor.
Approval Evidence
1 source6 linked approval claimsfirst-pass slug ps-intein
We introduce a photoswitchable intein (PS Intein) engineered by allosterically modulating a small autocatalytic gp41-1 intein with tandem Vivid photoreceptor.
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applicationsupports
PS Intein-based systems can be used to regulate gene expression and cell fate.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
Source:
applicationsupports
The high responsiveness and multi-input integration capability of PS Intein allow intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
The high responsiveness and ability to integrate multiple inputs allow for intersectional cell targeting using cancer- and tumor microenvironment-specific promoters.
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comparative performancesupports
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
PS Intein exhibits superior functionality and low background in cells compared to existing tools.
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compatibilitysupports
PS Intein tolerates various fusions and insertions, facilitating application in diverse cellular contexts.
PS Intein tolerates various fusions and insertions, facilitating its application in diverse cellular contexts.
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functional capabilitysupports
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins.
PS Intein-based systems enable light-induced covalent binding, cleavage, and release of proteins for regulating gene expression and cell fate.
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tool introductionsupports
This paper introduces PS Intein, a photoswitchable intein engineered by allosterically modulating gp41-1 intein with tandem Vivid photoreceptor.
We introduce a photoswitchable intein (PS Intein) engineered by allosterically modulating a small autocatalytic gp41-1 intein with tandem Vivid photoreceptor.
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Comparisons
Source-backed strengths
The reported strengths are high responsiveness and multi-input integration capability in a photoswitchable intein format. The source further supports cellular use cases including regulation of gene expression, cell fate, and intersectional cell targeting.
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PS Intein exhibits superior functionality and low background in cells compared to existing tools.
Ranked Citations
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