Source 1primary paper2025MED
Toolkit/rapamycin-inducible FKBP-FRB dimerization system
rapamycin-inducible FKBP-FRB dimerization system
Also known as: FKBP-FRB dimerization system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
here we employed a rapamycin-inducible FKBP-FRB dimerization system to artificially tether CAP-D2 and SMC4 either between different complexes (inter-complex tethering) or within single complexes (intra-complex tethering)
Usefulness & Problems
No literature-backed usefulness or problem-fit explainer has been materialized for this record yet.
Published Workflows
Condensin-Condensin Interactions Facilitate Mitotic Chromosome Assembly in Xenopus Egg Extracts.
2025Objective: Test whether hypothesized condensin-condensin interactions contribute to mitotic chromosome assembly by artificially tethering CAP-D2 and SMC4 and assessing chromosome assembly in Xenopus egg extracts.
Why it works: The workflow perturbs the proposed CAP-D2-SMC4 interaction directly by enforced tethering and then reads out chromosome assembly in Xenopus egg extracts, allowing the authors to test whether promoting or constraining this contact changes condensin function.
condensin-condensin interactionsCAP-D2-SMC4 contactrapamycin-inducible FKBP-FRB dimerizationXenopus egg extract chromosome assembly assay
Stages
- 1.Artificial tethering design and generation(library_design)
This stage creates the specific perturbations needed to distinguish inter-complex from intra-complex tethering effects on condensin function.
Selection: Configure CAP-D2 and SMC4 tethering either between different complexes or within single complexes using a rapamycin-inducible FKBP-FRB system.
- 2.Functional assessment in Xenopus egg extracts(confirmatory_validation)
This stage tests whether the engineered tethering perturbations alter the biological function relevant to the hypothesis.
Selection: Ability of the resulting complexes to assemble mitotic chromosomes in Xenopus egg extracts.
Steps
- 1.Apply rapamycin-inducible FKBP-FRB tethering to CAP-D2 and SMC4inducible tethering system
Create enforced CAP-D2-SMC4 contacts in either inter-complex or intra-complex configurations.
The tethering perturbation must be established before chromosome assembly can be tested.
- 2.Assess mitotic chromosome assembly of tethered complexes in Xenopus egg extracts
Determine how inter-complex versus intra-complex tethering affects condensin loading and chromosome assembly.
Functional assessment follows tether generation because the study aims to measure the consequences of the engineered perturbations.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
HeterodimerizationTechniques
Functional AssayTarget processes
No target processes tagged yet.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Intra-complex tethering of CAP-D2 and SMC4 restricts condensin function in the Xenopus egg extract chromosome assembly assay.
whereas intra-complex tethering restricts its function
Inter-complex tethering of CAP-D2 and SMC4 enhances condensin I loading and facilitates mitotic chromosome assembly in Xenopus egg extracts.
We found that inter-complex tethering enhances condensin I loading and facilitates mitotic chromosome assembly
Inter-complex tethering partially compensates for deficiencies in the D2-SMC4 contact caused by an SMC4 W-loop mutation.
deficiencies in the D2-SMC4 contact caused by an SMC4 W-loop mutation were partially compensated by inter-complex tethering
Approval Evidence
1 source3 linked approval claimsfirst-pass slug rapamycin-inducible-fkbp-frb-dimerization-system
here we employed a rapamycin-inducible FKBP-FRB dimerization system to artificially tether CAP-D2 and SMC4 either between different complexes (inter-complex tethering) or within single complexes (intra-complex tethering)
Source:
functional effectsupports
Intra-complex tethering of CAP-D2 and SMC4 restricts condensin function in the Xenopus egg extract chromosome assembly assay.
whereas intra-complex tethering restricts its function
Source:
mechanistic effectsupports
Inter-complex tethering of CAP-D2 and SMC4 enhances condensin I loading and facilitates mitotic chromosome assembly in Xenopus egg extracts.
We found that inter-complex tethering enhances condensin I loading and facilitates mitotic chromosome assembly
Source:
rescue effectsupports
Inter-complex tethering partially compensates for deficiencies in the D2-SMC4 contact caused by an SMC4 W-loop mutation.
deficiencies in the D2-SMC4 contact caused by an SMC4 W-loop mutation were partially compensated by inter-complex tethering
Source:
Comparisons
No literature-backed comparison notes have been materialized for this record yet.
Ranked Citations
- 1.