Source 1review2000Allergy
Toolkit/RAST inhibition
RAST inhibition
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Using RAST and RAST inhibition, Barnett et al. ( 1) showed that peanut-allergic individuals had extensive IgE binding to other legumes, including soy.
Usefulness & Problems
Why this is useful
RAST inhibition is described as an in vitro immunologic approach used to show extensive IgE binding to other legumes in peanut-allergic individuals. In this review it functions as evidence for antigenic similarity rather than definitive clinical diagnosis.; measuring IgE binding cross-reactivity among legumes; probing shared antigenicity between peanut and soy
Source:
RAST inhibition is described as an in vitro immunologic approach used to show extensive IgE binding to other legumes in peanut-allergic individuals. In this review it functions as evidence for antigenic similarity rather than definitive clinical diagnosis.
Source:
measuring IgE binding cross-reactivity among legumes
Source:
probing shared antigenicity between peanut and soy
Problem solved
It helps identify whether antibodies recognize related legume proteins such as soy.; detects serologic cross-reactivity that may underlie multiple positive allergy tests
Source:
It helps identify whether antibodies recognize related legume proteins such as soy.
Source:
detects serologic cross-reactivity that may underlie multiple positive allergy tests
Problem links
detects serologic cross-reactivity that may underlie multiple positive allergy tests
LiteratureIt helps identify whether antibodies recognize related legume proteins such as soy.
Source:
It helps identify whether antibodies recognize related legume proteins such as soy.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
competitive inhibition of ige bindingige-antigen bindingserologic cross-reactivity detectionTechniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The method requires allergic patient sera and assay reagents for measuring IgE binding and inhibition.; requires patient sera and immunologic assay infrastructure
The review explicitly indicates that such in vitro findings alone do not establish clinically important cross-reactivity.; the review indicates that in vitro cross-reactivity did not necessarily predict clinical coreactivity
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Elimination of all legumes in individuals with clinical reactions to one legume is generally unwarranted despite frequent multiple positive legume tests.
Epitope analysis suggests that linear IgE-binding epitopes are prominent in major peanut allergens and that some single amino-acid substitutions can reduce IgE binding, implying therapeutic potential.
Molecular studies indicate that peanut and soy contain both homologous and unique allergenic proteins, helping explain why serologic cross-reactivity does not always produce clinical coallergy.
Serologic or skin-test cross-reactivity between peanut and soy is common, but clinically important peanut-soy coallergy is uncommon.
clinical soy reactivity in peanut allergic children 3%coallergy rate atopic dermatitis cohort 1 0.8%coallergy rate atopic dermatitis cohort 2 1.8%soy reactivity among severe peanut allergy cases 6.5%
Approval Evidence
1 source1 linked approval claimfirst-pass slug rast-inhibition
Using RAST and RAST inhibition, Barnett et al. ( 1) showed that peanut-allergic individuals had extensive IgE binding to other legumes, including soy.
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review summarysupports
Serologic or skin-test cross-reactivity between peanut and soy is common, but clinically important peanut-soy coallergy is uncommon.
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Comparisons
Source-stated alternatives
The review contrasts these in vitro studies with oral food challenges and clinical observation.
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The review contrasts these in vitro studies with oral food challenges and clinical observation.
Source-backed strengths
used in the review as evidence for extensive IgE binding to other legumes including soy
Source:
used in the review as evidence for extensive IgE binding to other legumes including soy
Compared with Oral food challenge
The review contrasts these in vitro studies with oral food challenges and clinical observation.
Shared frame: source-stated alternative in extracted literature
Strengths here: used in the review as evidence for extensive IgE binding to other legumes including soy.
Relative tradeoffs: the review indicates that in vitro cross-reactivity did not necessarily predict clinical coreactivity.
Source:
The review contrasts these in vitro studies with oral food challenges and clinical observation.
Ranked Citations
- 1.