Source 1primary paper2021Nature Communications
Toolkit/reversible phosphorylation-dependent switch for membrane targeting
reversible phosphorylation-dependent switch for membrane targeting
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
This tool is a de novo designed reversible phosphorylation-dependent switch for membrane targeting. The supplied evidence indicates that it links phosphorylation state to reversible membrane localization, but does not provide additional molecular or performance details.
Usefulness & Problems
Why this is useful
This switch is useful as a designed system for coupling phosphorylation inputs to changes in membrane targeting. The available evidence supports its conceptual utility for reversible control of subcellular localization, but does not specify experimental contexts or application domains.
Problem solved
It addresses the problem of engineering a reversible, phosphorylation-responsive mechanism for membrane targeting. The supplied source does not further define the biological setting, target membrane, or signaling pathway constraints.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
Computational DesignTarget processes
No target processes tagged yet.
Implementation Constraints
The only implementation detail supported by the evidence is that the switch was generated by de novo design. No information is provided on construct composition, required cofactors, expression system, delivery method, or assay format.
The evidence is limited to the title-level claim that such a switch was designed. There are no supplied details on component architecture, phosphorylation sites, cognate kinases or phosphatases, membrane specificity, host organism, or experimental validation breadth.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The paper reports de novo design of a reversible phosphorylation-dependent switch for membrane targeting.
The paper reports de novo design of a reversible phosphorylation-dependent switch for membrane targeting.
The paper reports de novo design of a reversible phosphorylation-dependent switch for membrane targeting.
The paper reports de novo design of a reversible phosphorylation-dependent switch for membrane targeting.
The paper reports de novo design of a reversible phosphorylation-dependent switch for membrane targeting.
The paper reports de novo design of a reversible phosphorylation-dependent switch for membrane targeting.
The paper reports de novo design of a reversible phosphorylation-dependent switch for membrane targeting.
Approval Evidence
1 source1 linked approval claimfirst-pass slug reversible-phosphorylation-dependent-switch-for-membrane-targeting
De novo design of a reversible phosphorylation-dependent switch for membrane targeting
Source:
design reportsupports
The paper reports de novo design of a reversible phosphorylation-dependent switch for membrane targeting.
Source:
Comparisons
Source-backed strengths
A key reported strength is that the system was de novo designed and is reversible in its phosphorylation-dependent membrane targeting behavior. No quantitative performance metrics, dynamic range, kinetics, or validation results are provided in the supplied evidence.
Ranked Citations
- 1.