RNA interference

Promoter engineering and Prime Editing are expected to improve precision of soybean genetic modification and minimize pleiotropic effects.

The integration of new approaches, such as promoter engineering and Prime Editing, promises to further enhance the precision of genetic modifications, minimizing pleiotropic effects.

Editing SWEET10a and SWEET10b allows modulation of the soybean oil-protein balance.

the editing of sugar transporters SWEET10a and SWEET10b allows the modulation of the oil-protein balance

Inactivation of genes related to antinutritional factors has reduced expression of phytate and protease inhibitors in soybean.

Simultaneously, the inactivation of genes related to antinutritional factors has significantly reduced the expression of compounds such as phytate and protease inhibitors.

Silencing negative regulatory genes such as CIF1 and AIP2 can elevate soybean seed protein content.

Recent studies demonstrate that the silencing of negative regulatory genes, such as CIF1 and AIP2, can elevate the protein content of seeds

RNAi, CRISPR/Cas9, and AlphaFold2-guided gene editing are used to modify genes involved in carbon and nitrogen metabolism and storage proteins in soybean.

This work reviews the main progress achieved through transgenesis, induced mutagenesis, and precision gene editing, highlighting the role of tools such as RNAi, CRISPR/Cas9, and AlphaFold2-guided gene editing in modifying genes involved in carbon and nitrogen metabolism and storage proteins.

AAVs and lentiviruses have been used for gene delivery in preclinical and clinical studies.

Adeno-associated viruses (AAVs) and lentiviruses have been used for gene delivery in preclinical and clinical studies

MSC-based paracrine support and transplantation of neurons derived from iPSCs are being evaluated as cellular therapies, particularly in Parkinson's disease and Alzheimer's disease.

Cellular therapies, including mesenchymal stem cell (MSC)-based paracrine support and transplantation of neurons derived from induced pluripotent stem cells (iPSCs), are being evaluated, particularly in PD and AD.

CRISPR/Cas9-mediated gene editing enables precise genetic modification in soybean and has produced improved oil composition, increased isoflavone content, and resistance to biotic stresses.

Isothermal amplification, CRISPR/Cas-hybrid assays, and next-generation sequencing can achieve sensitive detection of tobamoviruses in the field with minimal sample preparation.

Marker-assisted selection using SSRs and SNPs facilitates efficient identification and incorporation of desired soybean traits including disease resistance, abiotic stress tolerance, and improved seed quality.

RNA interference modulates gene expression in soybean to optimize nutritional properties and stress responses.

These molecular breeding approaches overcome limitations of traditional methods by shortening the breeding cycle and allowing simultaneous improvement of multiple traits.

Integrating CRISPR-based molecular diagnostics, omics technologies, designed protective systems, and climate-augmented disease prediction offers a blueprint for sustainable control of tobamoviruses and crop protection.

ASOs are under development to reduce expression of pathogenic proteins such as tau, α-synuclein, and mutant huntingtin.

ASOs are under development to reduce expression of pathogenic proteins such as tau, α-synuclein, and mutant huntingtin.

Small RNA profiling and network analyses of viral movement proteins reveal complex mechanisms of immune evasion and resistance breakdown.

Genomic selection improves prediction accuracy for complex quantitative soybean traits such as yield by integrating genome-wide molecular markers with phenotypic data.

Current and developing therapeutic strategies for neurodegeneration include viral vector-based gene delivery, antisense oligonucleotide and RNA interference methods, stem cell transplantation, and genome editing technologies.

In this review, we describe current and developing therapeutic strategies that include viral vector-based gene delivery, antisense oligonucleotide (ASO) and RNA interference methods, stem cell transplantation, and genome editing technologies.

Genome editing with CRISPR, RNA interference, and multi-omics approaches can facilitate real-time surveillance and breeding for enhanced resilience.

The reviewed targeted gene-silencing systems are applied in eukaryotes including plants and animals.

The review considers advantages and disadvantages of each targeted gene-silencing approach, compares their effectiveness, and discusses usage peculiarities in plant and animal organisms.

The review describes RNA interference, chimeric transcription factors, chimeric zinc finger proteins, TALE-based repressors, optogenetic tools, and CRISPR/Cas-based repressors as main systems for targeted suppression of gene expression.

Gene-therapy modalities including ASOs, RNAi, CRISPR, and virus-based delivery systems have played crucial roles in discovering and validating new pain targets.

The review covers ASOs, siRNAs, optogenetics, chemogenetics, CRISPR, and their delivery methods targeting primary sensory neurons and non-neuronal cells including glia and chondrocytes.

Although gene therapy-based clinical trials have increased, trials focused on pain as the primary outcome remain uncommon.

Most cockroaches remained rhythmically active after each clock gene knockdown, with weakened rhythms after per RNAi and shorter periods after tim1 RNAi and cry2 RNAi.

Unexpectedly, circadian locomotor activity of most cockroaches remained rhythmic for each clock gene knockdown employed. It expressed weakened rhythms and unchanged periods for Rm´perRNAi and shorter periods for Rm´tim1RNAi and Rm´cry2RNAi.

Most cockroaches remained rhythmically active after each clock gene knockdown, with weakened rhythms after per RNAi and shorter periods after tim1 RNAi and cry2 RNAi.

Unexpectedly, circadian locomotor activity of most cockroaches remained rhythmic for each clock gene knockdown employed. It expressed weakened rhythms and unchanged periods for Rm´perRNAi and shorter periods for Rm´tim1RNAi and Rm´cry2RNAi.

Most cockroaches remained rhythmically active after each clock gene knockdown, with weakened rhythms after per RNAi and shorter periods after tim1 RNAi and cry2 RNAi.

Unexpectedly, circadian locomotor activity of most cockroaches remained rhythmic for each clock gene knockdown employed. It expressed weakened rhythms and unchanged periods for Rm´perRNAi and shorter periods for Rm´tim1RNAi and Rm´cry2RNAi.

Most cockroaches remained rhythmically active after each clock gene knockdown, with weakened rhythms after per RNAi and shorter periods after tim1 RNAi and cry2 RNAi.

Unexpectedly, circadian locomotor activity of most cockroaches remained rhythmic for each clock gene knockdown employed. It expressed weakened rhythms and unchanged periods for Rm´perRNAi and shorter periods for Rm´tim1RNAi and Rm´cry2RNAi.

Most cockroaches remained rhythmically active after each clock gene knockdown, with weakened rhythms after per RNAi and shorter periods after tim1 RNAi and cry2 RNAi.

Unexpectedly, circadian locomotor activity of most cockroaches remained rhythmic for each clock gene knockdown employed. It expressed weakened rhythms and unchanged periods for Rm´perRNAi and shorter periods for Rm´tim1RNAi and Rm´cry2RNAi.

Most cockroaches remained rhythmically active after each clock gene knockdown, with weakened rhythms after per RNAi and shorter periods after tim1 RNAi and cry2 RNAi.

Unexpectedly, circadian locomotor activity of most cockroaches remained rhythmic for each clock gene knockdown employed. It expressed weakened rhythms and unchanged periods for Rm´perRNAi and shorter periods for Rm´tim1RNAi and Rm´cry2RNAi.

Most cockroaches remained rhythmically active after each clock gene knockdown, with weakened rhythms after per RNAi and shorter periods after tim1 RNAi and cry2 RNAi.

Unexpectedly, circadian locomotor activity of most cockroaches remained rhythmic for each clock gene knockdown employed. It expressed weakened rhythms and unchanged periods for Rm´perRNAi and shorter periods for Rm´tim1RNAi and Rm´cry2RNAi.

Single dsRNA injections against each clock gene successfully and permanently knocked down the respective mRNA levels within about two weeks.

Single injections of dsRNA of each clock gene into adult cockroaches successfully and permanently knocked down respective mRNA levels within ~two weeks

Single dsRNA injections against each clock gene successfully and permanently knocked down the respective mRNA levels within about two weeks.

Single injections of dsRNA of each clock gene into adult cockroaches successfully and permanently knocked down respective mRNA levels within ~two weeks

Single dsRNA injections against each clock gene successfully and permanently knocked down the respective mRNA levels within about two weeks.

Single injections of dsRNA of each clock gene into adult cockroaches successfully and permanently knocked down respective mRNA levels within ~two weeks

Single dsRNA injections against each clock gene successfully and permanently knocked down the respective mRNA levels within about two weeks.

Single injections of dsRNA of each clock gene into adult cockroaches successfully and permanently knocked down respective mRNA levels within ~two weeks

Single dsRNA injections against each clock gene successfully and permanently knocked down the respective mRNA levels within about two weeks.

Single injections of dsRNA of each clock gene into adult cockroaches successfully and permanently knocked down respective mRNA levels within ~two weeks

Single dsRNA injections against each clock gene successfully and permanently knocked down the respective mRNA levels within about two weeks.

Single injections of dsRNA of each clock gene into adult cockroaches successfully and permanently knocked down respective mRNA levels within ~two weeks

Single dsRNA injections against each clock gene successfully and permanently knocked down the respective mRNA levels within about two weeks.

Single injections of dsRNA of each clock gene into adult cockroaches successfully and permanently knocked down respective mRNA levels within ~two weeks

The data were consistent with two synchronized groups of coupled oscillator cells, a leading morning oscillator and a lagging evening oscillator, coupled via mutual inhibition.

Data were consistent with two synchronized main groups of coupled oscillator cells, a leading (morning) oscillator, or a lagging (evening) oscillator that couple via mutual inhibition.

The data were consistent with two synchronized groups of coupled oscillator cells, a leading morning oscillator and a lagging evening oscillator, coupled via mutual inhibition.

Data were consistent with two synchronized main groups of coupled oscillator cells, a leading (morning) oscillator, or a lagging (evening) oscillator that couple via mutual inhibition.

The data were consistent with two synchronized groups of coupled oscillator cells, a leading morning oscillator and a lagging evening oscillator, coupled via mutual inhibition.

Data were consistent with two synchronized main groups of coupled oscillator cells, a leading (morning) oscillator, or a lagging (evening) oscillator that couple via mutual inhibition.

The data were consistent with two synchronized groups of coupled oscillator cells, a leading morning oscillator and a lagging evening oscillator, coupled via mutual inhibition.

Data were consistent with two synchronized main groups of coupled oscillator cells, a leading (morning) oscillator, or a lagging (evening) oscillator that couple via mutual inhibition.

The data were consistent with two synchronized groups of coupled oscillator cells, a leading morning oscillator and a lagging evening oscillator, coupled via mutual inhibition.

Data were consistent with two synchronized main groups of coupled oscillator cells, a leading (morning) oscillator, or a lagging (evening) oscillator that couple via mutual inhibition.

The data were consistent with two synchronized groups of coupled oscillator cells, a leading morning oscillator and a lagging evening oscillator, coupled via mutual inhibition.

Data were consistent with two synchronized main groups of coupled oscillator cells, a leading (morning) oscillator, or a lagging (evening) oscillator that couple via mutual inhibition.

The data were consistent with two synchronized groups of coupled oscillator cells, a leading morning oscillator and a lagging evening oscillator, coupled via mutual inhibition.

Data were consistent with two synchronized main groups of coupled oscillator cells, a leading (morning) oscillator, or a lagging (evening) oscillator that couple via mutual inhibition.

Modelling suggests an additional negative feedback exists next to Rm-PER in cockroach morning oscillator cells.

Modelling suggests that there is an additional negative feedback next to Rm´PER in cockroach morning oscillator cells.

Modelling suggests an additional negative feedback exists next to Rm-PER in cockroach morning oscillator cells.

Modelling suggests that there is an additional negative feedback next to Rm´PER in cockroach morning oscillator cells.

Modelling suggests an additional negative feedback exists next to Rm-PER in cockroach morning oscillator cells.

Modelling suggests that there is an additional negative feedback next to Rm´PER in cockroach morning oscillator cells.

Modelling suggests an additional negative feedback exists next to Rm-PER in cockroach morning oscillator cells.

Modelling suggests that there is an additional negative feedback next to Rm´PER in cockroach morning oscillator cells.

Modelling suggests an additional negative feedback exists next to Rm-PER in cockroach morning oscillator cells.

Modelling suggests that there is an additional negative feedback next to Rm´PER in cockroach morning oscillator cells.

Modelling suggests an additional negative feedback exists next to Rm-PER in cockroach morning oscillator cells.

Modelling suggests that there is an additional negative feedback next to Rm´PER in cockroach morning oscillator cells.

Modelling suggests an additional negative feedback exists next to Rm-PER in cockroach morning oscillator cells.

Modelling suggests that there is an additional negative feedback next to Rm´PER in cockroach morning oscillator cells.

Neither per, tim1, nor cry2 alone is an essential component of the molecular circadian clockwork in the Madeira cockroach.

Neither per, nor tim1, nor cry2 alone are essential components of the molecular circadian clockwork in the Madeira cockroach

Neither per, tim1, nor cry2 alone is an essential component of the molecular circadian clockwork in the Madeira cockroach.

Neither per, nor tim1, nor cry2 alone are essential components of the molecular circadian clockwork in the Madeira cockroach

Neither per, tim1, nor cry2 alone is an essential component of the molecular circadian clockwork in the Madeira cockroach.

Neither per, nor tim1, nor cry2 alone are essential components of the molecular circadian clockwork in the Madeira cockroach

Neither per, tim1, nor cry2 alone is an essential component of the molecular circadian clockwork in the Madeira cockroach.

Neither per, nor tim1, nor cry2 alone are essential components of the molecular circadian clockwork in the Madeira cockroach

Neither per, tim1, nor cry2 alone is an essential component of the molecular circadian clockwork in the Madeira cockroach.

Neither per, nor tim1, nor cry2 alone are essential components of the molecular circadian clockwork in the Madeira cockroach

Neither per, tim1, nor cry2 alone is an essential component of the molecular circadian clockwork in the Madeira cockroach.

Neither per, nor tim1, nor cry2 alone are essential components of the molecular circadian clockwork in the Madeira cockroach

Neither per, tim1, nor cry2 alone is an essential component of the molecular circadian clockwork in the Madeira cockroach.

Neither per, nor tim1, nor cry2 alone are essential components of the molecular circadian clockwork in the Madeira cockroach

CRISPR and RNA interference are relevant screening modalities associated with this review's topic of genetic screening approaches.

OpenAlex concepts include "RNA interference" and "CRISPR"; title includes "genetic screening approaches".

Gene therapy can result in stable or inducible transgene expression and can allow nearly specific expression in target cells.

HSV-1-derived viral vectors are presented as potential tools for simultaneous delivery and expression of multiple transgene cassettes.

Cell-specific and inducible promoters allow gene products to be expressed only in specific cells and allow control of transcriptional activation.

DREADDs are presented as systems allowing spatial or temporal control of expression in CNS disease applications.

RNA interference is presented as a post-transcriptional regulation system applied to CNS diseases.

CRISPR/Cas9 and zinc finger proteins are included as gene-editing technologies relevant to CNS disease applications.

Silencing negative regulatory genes such as CIF1 and AIP2 can elevate soybean seed protein content.

Recent studies demonstrate that the silencing of negative regulatory genes, such as CIF1 and AIP2, can elevate the protein content of seeds

Source: Metabolic engineering of soybean for improving grain quality for animal consumption.

RNAi, CRISPR/Cas9, and AlphaFold2-guided gene editing are used to modify genes involved in carbon and nitrogen metabolism and storage proteins in soybean.

This work reviews the main progress achieved through transgenesis, induced mutagenesis, and precision gene editing, highlighting the role of tools such as RNAi, CRISPR/Cas9, and AlphaFold2-guided gene editing in modifying genes involved in carbon and nitrogen metabolism and storage proteins.

Source: Metabolic engineering of soybean for improving grain quality for animal consumption.

RNA interference modulates gene expression in soybean to optimize nutritional properties and stress responses.

Source: Advancements in Molecular Breeding Techniques for Soybeans.

These molecular breeding approaches overcome limitations of traditional methods by shortening the breeding cycle and allowing simultaneous improvement of multiple traits.

Source: Advancements in Molecular Breeding Techniques for Soybeans.

Current and developing therapeutic strategies for neurodegeneration include viral vector-based gene delivery, antisense oligonucleotide and RNA interference methods, stem cell transplantation, and genome editing technologies.

In this review, we describe current and developing therapeutic strategies that include viral vector-based gene delivery, antisense oligonucleotide (ASO) and RNA interference methods, stem cell transplantation, and genome editing technologies.

Source: Viral and non-viral cellular therapies for neurodegeneration.

Genome editing with CRISPR, RNA interference, and multi-omics approaches can facilitate real-time surveillance and breeding for enhanced resilience.

Source: Advanced molecular tools for surveillance and management of tobamoviruses.

The reviewed targeted gene-silencing systems are applied in eukaryotes including plants and animals.

Source: Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals

The review considers advantages and disadvantages of each targeted gene-silencing approach, compares their effectiveness, and discusses usage peculiarities in plant and animal organisms.

Source: Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals

The review describes RNA interference, chimeric transcription factors, chimeric zinc finger proteins, TALE-based repressors, optogenetic tools, and CRISPR/Cas-based repressors as main systems for targeted suppression of gene expression.

Source: Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals

Gene-therapy modalities including ASOs, RNAi, CRISPR, and virus-based delivery systems have played crucial roles in discovering and validating new pain targets.

Source: Gene therapy for chronic pain management

Although gene therapy-based clinical trials have increased, trials focused on pain as the primary outcome remain uncommon.

Source: Gene therapy for chronic pain management

Most cockroaches remained rhythmically active after each clock gene knockdown, with weakened rhythms after per RNAi and shorter periods after tim1 RNAi and cry2 RNAi.

Unexpectedly, circadian locomotor activity of most cockroaches remained rhythmic for each clock gene knockdown employed. It expressed weakened rhythms and unchanged periods for Rm´perRNAi and shorter periods for Rm´tim1RNAi and Rm´cry2RNAi.

Source: Neither per, nor tim1, nor cry2 alone are essential components of the molecular circadian clockwork in the Madeira cockroach

Single dsRNA injections against each clock gene successfully and permanently knocked down the respective mRNA levels within about two weeks.

Single injections of dsRNA of each clock gene into adult cockroaches successfully and permanently knocked down respective mRNA levels within ~two weeks

Source: Neither per, nor tim1, nor cry2 alone are essential components of the molecular circadian clockwork in the Madeira cockroach

Neither per, tim1, nor cry2 alone is an essential component of the molecular circadian clockwork in the Madeira cockroach.

Neither per, nor tim1, nor cry2 alone are essential components of the molecular circadian clockwork in the Madeira cockroach

Source: Neither per, nor tim1, nor cry2 alone are essential components of the molecular circadian clockwork in the Madeira cockroach

CRISPR and RNA interference are relevant screening modalities associated with this review's topic of genetic screening approaches.

OpenAlex concepts include "RNA interference" and "CRISPR"; title includes "genetic screening approaches".

Source: ER-mitochondria contact sites in neurodegeneration: genetic screening approaches to investigate novel disease mechanisms

RNA interference is presented as a post-transcriptional regulation system applied to CNS diseases.

Source: Gene Therapy Tools for Brain Diseases