Source 1review2010Biopolymers
Toolkit/sequence independent DNA stains with inducible photoblinking
sequence independent DNA stains with inducible photoblinking
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
A high labeling density of photoswitchable fluorophores is crucial for these techniques, which can be provided by sequence independent DNA stains in which photoblinking reactions can be induced.
Usefulness & Problems
No literature-backed usefulness or problem-fit explainer has been materialized for this record yet.
Published Workflows
Objective: Obtain super-resolution images of isolated DNA or chromatin by combining dense photoswitchable labeling with single-molecule localization microscopy.
Why it works: The review states that high labeling density of photoswitchable fluorophores is crucial, and describes DNA stains and cyanine dyes whose induced photoblinking supports localization-based reconstruction.
photoblinking of DNA-bound fluorophoresDNA intercalationminor-groove DNA bindingsingle-molecule localization microscopysuper-resolution fluorescence microscopy
Stages
- 1.High-density photoswitchable DNA labeling(library_design)
The review states that high labeling density of photoswitchable fluorophores is crucial for single-molecule localization techniques.
Selection: Choose labeling approaches that provide high labeling density of photoswitchable fluorophores on DNA.
- 2.Induce photoblinking in DNA stains or cyanine dyes(functional_characterization)
The abstract links induced photoblinking reactions to the ability of sequence-independent DNA stains to support these techniques.
Selection: Use DNA stains or cyanine dyes under conditions that induce photoblinking suitable for localization microscopy.
- 3.Single-molecule localization imaging of isolated DNA or chromatin(confirmatory_validation)
This is the stage where the labeling and photoblinking strategy is converted into nanoscale optical images of DNA organization.
Selection: Apply single-molecule localization super-resolution imaging to labeled isolated DNA or chromatin samples.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Techniques
No technique tags yet.
Target processes
localizationInput: Light
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Rapid progress in labeling, optics, and single-molecule localization algorithms is likely to improve insight into DNA structuring in cells and materials.
High labeling density of photoswitchable fluorophores is crucial for single-molecule localization super-resolution imaging of DNA.
Approval Evidence
1 source1 linked approval claimfirst-pass slug sequence-independent-dna-stains-with-inducible-photoblinking
A high labeling density of photoswitchable fluorophores is crucial for these techniques, which can be provided by sequence independent DNA stains in which photoblinking reactions can be induced.
Source:
requirementsupports
High labeling density of photoswitchable fluorophores is crucial for single-molecule localization super-resolution imaging of DNA.
Source:
Comparisons
No literature-backed comparison notes have been materialized for this record yet.
Ranked Citations
- 1.