Source 1primary paper2006Blood
Toolkit/shRNA-delivered by lentivirus
shRNA-delivered by lentivirus
Also known as: RNA interference with an shRNA-delivered by lentivirus
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
shRNA delivered by lentivirus was used as an RNA interference-based perturbation method to reduce endogenous CIB1 levels in endothelial cells. In the cited study, this knockdown approach was applied to test CIB1 function in endothelial haptotaxis and tube formation.
Usefulness & Problems
Why this is useful
This method is useful for stable or efficient genetic loss-of-function perturbation in endothelial cells when depletion of an endogenous target such as CIB1 is required. In the cited work, it enabled functional interrogation of CIB1-dependent endothelial behaviors relevant to angiogenesis.
Source:
CIB1 depletion significantly decreased EC haptotaxis on fibronectin and EC vascular tube formation on growth factor-reduced Matrigel.
Problem solved
It addresses the problem of reducing endogenous CIB1 expression in endothelial cells to determine how CIB1 contributes to cell haptotaxis on fibronectin and vascular tube formation on growth factor-reduced Matrigel. The study further used this perturbation to test whether FGF-2 could overcome the effects of CIB1 depletion.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Techniques
No technique tags yet.
Target processes
recombinationImplementation Constraints
The available evidence states only that endogenous CIB1 levels in endothelial cells were reduced by RNA interference using an shRNA delivered by lentivirus. No additional practical details are provided on promoter choice, lentiviral packaging system, multiplicity of infection, selection strategy, or construct architecture.
The supplied evidence documents use against CIB1 in endothelial cells but does not provide sequence design, knockdown efficiency, vector details, or comparison with alternative delivery methods. Validation is limited to one study and a narrow set of endothelial functional assays.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
CIB1 depletion decreases endothelial cell haptotaxis on fibronectin and vascular tube formation on growth factor-reduced Matrigel.
CIB1 depletion significantly decreased EC haptotaxis on fibronectin and EC vascular tube formation on growth factor-reduced Matrigel.
CIB1 depletion decreases endothelial cell haptotaxis on fibronectin and vascular tube formation on growth factor-reduced Matrigel.
CIB1 depletion significantly decreased EC haptotaxis on fibronectin and EC vascular tube formation on growth factor-reduced Matrigel.
CIB1 depletion decreases endothelial cell haptotaxis on fibronectin and vascular tube formation on growth factor-reduced Matrigel.
CIB1 depletion significantly decreased EC haptotaxis on fibronectin and EC vascular tube formation on growth factor-reduced Matrigel.
CIB1 depletion decreases endothelial cell haptotaxis on fibronectin and vascular tube formation on growth factor-reduced Matrigel.
CIB1 depletion significantly decreased EC haptotaxis on fibronectin and EC vascular tube formation on growth factor-reduced Matrigel.
CIB1 depletion decreases endothelial cell haptotaxis on fibronectin and vascular tube formation on growth factor-reduced Matrigel.
CIB1 depletion significantly decreased EC haptotaxis on fibronectin and EC vascular tube formation on growth factor-reduced Matrigel.
CIB1 depletion decreases endothelial cell haptotaxis on fibronectin and vascular tube formation on growth factor-reduced Matrigel.
CIB1 depletion significantly decreased EC haptotaxis on fibronectin and EC vascular tube formation on growth factor-reduced Matrigel.
CIB1 depletion decreases endothelial cell haptotaxis on fibronectin and vascular tube formation on growth factor-reduced Matrigel.
CIB1 depletion significantly decreased EC haptotaxis on fibronectin and EC vascular tube formation on growth factor-reduced Matrigel.
FGF-2 treatment did not counter inhibition of endothelial cell haptotaxis and tube formation caused by shRNA against CIB1.
Treatment with FGF-2, an angiogenic factor, did not counter the observed inhibition of haptotaxis and tube formation by shRNA against CIB1.
FGF-2 treatment did not counter inhibition of endothelial cell haptotaxis and tube formation caused by shRNA against CIB1.
Treatment with FGF-2, an angiogenic factor, did not counter the observed inhibition of haptotaxis and tube formation by shRNA against CIB1.
FGF-2 treatment did not counter inhibition of endothelial cell haptotaxis and tube formation caused by shRNA against CIB1.
Treatment with FGF-2, an angiogenic factor, did not counter the observed inhibition of haptotaxis and tube formation by shRNA against CIB1.
FGF-2 treatment did not counter inhibition of endothelial cell haptotaxis and tube formation caused by shRNA against CIB1.
Treatment with FGF-2, an angiogenic factor, did not counter the observed inhibition of haptotaxis and tube formation by shRNA against CIB1.
FGF-2 treatment did not counter inhibition of endothelial cell haptotaxis and tube formation caused by shRNA against CIB1.
Treatment with FGF-2, an angiogenic factor, did not counter the observed inhibition of haptotaxis and tube formation by shRNA against CIB1.
FGF-2 treatment did not counter inhibition of endothelial cell haptotaxis and tube formation caused by shRNA against CIB1.
Treatment with FGF-2, an angiogenic factor, did not counter the observed inhibition of haptotaxis and tube formation by shRNA against CIB1.
FGF-2 treatment did not counter inhibition of endothelial cell haptotaxis and tube formation caused by shRNA against CIB1.
Treatment with FGF-2, an angiogenic factor, did not counter the observed inhibition of haptotaxis and tube formation by shRNA against CIB1.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug shrna-delivered-by-lentivirus
we reduced endogenous CIB1 levels in ECs by RNA interference with an shRNA-delivered by lentivirus
Source:
functional effectsupports
CIB1 depletion decreases endothelial cell haptotaxis on fibronectin and vascular tube formation on growth factor-reduced Matrigel.
CIB1 depletion significantly decreased EC haptotaxis on fibronectin and EC vascular tube formation on growth factor-reduced Matrigel.
Source:
perturbation responsesupports
FGF-2 treatment did not counter inhibition of endothelial cell haptotaxis and tube formation caused by shRNA against CIB1.
Treatment with FGF-2, an angiogenic factor, did not counter the observed inhibition of haptotaxis and tube formation by shRNA against CIB1.
Source:
Comparisons
Source-backed strengths
In the cited study, lentiviral shRNA-mediated CIB1 depletion produced measurable phenotypic effects, decreasing endothelial cell haptotaxis on fibronectin and tube formation on growth factor-reduced Matrigel. The inhibitory phenotype persisted despite FGF-2 treatment, supporting a robust functional perturbation in those assays.
Ranked Citations
- 1.