Source 1primary paper2021
Toolkit/SIBR-Cas
SIBR-Cas
Also known as: Self-splicing Intron-Based Riboswitch-Cas
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
SIBR-Cas, termed Self-splicing Intron-Based Riboswitch-Cas, is a multi-component bacterial CRISPR genome engineering system that provides inducible control over CRISPR-Cas counterselection. It is reported to delay counterselection to permit editing events and has been applied to gene knockout in bacteria with poor homologous recombination systems.
Usefulness & Problems
Why this is useful
SIBR-Cas is useful for bacterial genome engineering because it provides inducible and reportedly tight regulation of CRISPR-Cas counterselection. The cited study positions it as a simple and widely applicable approach for non-model bacteria, including hosts with poor homologous recombination systems.
Source:
Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
Source:
developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Problem solved
This system addresses the problem that immediate CRISPR-Cas counterselection can prevent recovery of edited cells before recombination occurs. It is specifically presented as enabling gene knockout in bacteria with poor homologous recombination capacity without exogenous recombinases.
Source:
Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
inducible control of crispr-cas counterselectionriboswitch-mediated regulationself-splicing intron-based regulationtemporal delay of crispr-cas counterselectionTechniques
Selection / EnrichmentTarget processes
editingrecombinationselectionImplementation Constraints
The available evidence indicates that SIBR-Cas is a multi-component system built around a self-splicing intron-based riboswitch for inducible control of CRISPR-Cas counterselection. It was used for bacterial gene knockout without exogenous recombinases, but the supplied evidence does not specify the Cas protein, intron architecture, inducer, construct design, or delivery method.
The supplied evidence does not provide quantitative editing efficiencies, leakiness measurements, inducer identity, or direct comparisons against specific alternative systems. Validation is described from a single 2021 source, so independent replication and breadth across diverse bacterial taxa are not established here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successBacteriaapplication demo
Inferred from claim c4 during normalization. Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems. Derived from claim c4. Quoted text: Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
Source:
number of bacteria3
successBacteriaapplication demo
Inferred from claim c4 during normalization. Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems. Derived from claim c4. Quoted text: Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
Source:
number of bacteria3
successBacteriaapplication demo
Inferred from claim c4 during normalization. Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems. Derived from claim c4. Quoted text: Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
Source:
number of bacteria3
successBacteriaapplication demo
Inferred from claim c4 during normalization. Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems. Derived from claim c4. Quoted text: Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
Source:
number of bacteria3
successBacteriaapplication demo
Inferred from claim c4 during normalization. Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems. Derived from claim c4. Quoted text: Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
Source:
number of bacteria3
successBacteriaapplication demo
Inferred from claim c4 during normalization. Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems. Derived from claim c4. Quoted text: Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
Source:
number of bacteria3
successBacteriaapplication demo
Inferred from claim c4 during normalization. Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems. Derived from claim c4. Quoted text: Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
Source:
number of bacteria3
Supporting Sources
Ranked Claims
Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.
Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.
Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.
Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.
Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.
Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.
Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.
Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.
Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.
Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.
Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.
Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.
Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.
Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.
Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.
This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.
This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.
This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.
This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.
This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.
This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.
This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.
allows for universal and inducible control over CRISPR-Cas counterselection
SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.
allows for universal and inducible control over CRISPR-Cas counterselection
SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.
allows for universal and inducible control over CRISPR-Cas counterselection
SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.
allows for universal and inducible control over CRISPR-Cas counterselection
SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.
allows for universal and inducible control over CRISPR-Cas counterselection
SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.
allows for universal and inducible control over CRISPR-Cas counterselection
SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.
allows for universal and inducible control over CRISPR-Cas counterselection
SIBR is proposed as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria.
we propose that SIBR can have a wider application as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria
SIBR is proposed as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria.
we propose that SIBR can have a wider application as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria
SIBR is proposed as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria.
we propose that SIBR can have a wider application as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria
SIBR is proposed as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria.
we propose that SIBR can have a wider application as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria
SIBR is proposed as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria.
we propose that SIBR can have a wider application as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria
SIBR is proposed as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria.
we propose that SIBR can have a wider application as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria
SIBR is proposed as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria.
we propose that SIBR can have a wider application as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria
SIBR-Cas is a simple and universal genome engineering tool for bacteria.
developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
SIBR-Cas is a simple and universal genome engineering tool for bacteria.
developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
SIBR-Cas is a simple and universal genome engineering tool for bacteria.
developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
SIBR-Cas is a simple and universal genome engineering tool for bacteria.
developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
SIBR-Cas is a simple and universal genome engineering tool for bacteria.
developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
SIBR-Cas is a simple and universal genome engineering tool for bacteria.
developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
SIBR-Cas is a simple and universal genome engineering tool for bacteria.
developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Approval Evidence
1 source5 linked approval claimsfirst-pass slug sibr-cas
we termed SIBR-Cas (Self-splicing Intron-Based Riboswitch-Cas)
Source:
application resultsupports
Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.
Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
Source:
comparative advantagesupports
Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.
Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Source:
mechanismsupports
Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.
This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Source:
mechanismsupports
SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.
allows for universal and inducible control over CRISPR-Cas counterselection
Source:
tool descriptionsupports
SIBR-Cas is a simple and universal genome engineering tool for bacteria.
developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Source:
Comparisons
Source-backed strengths
The reported strengths are simplicity, tight regulation, and broad applicability across most non-model bacteria. In the cited work, SIBR-Cas was successfully used without exogenous recombinases to knock out several genes in three bacterial species with poor homologous recombination systems.
Source:
Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Ranked Citations
- 1.