Toolkit/SIBR-Cas

SIBR-Cas

Multi-Component Switch·Research·Since 2021

Also known as: Self-splicing Intron-Based Riboswitch-Cas

Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

SIBR-Cas, termed Self-splicing Intron-Based Riboswitch-Cas, is a multi-component bacterial CRISPR genome engineering system that provides inducible control over CRISPR-Cas counterselection. It is reported to delay counterselection to permit editing events and has been applied to gene knockout in bacteria with poor homologous recombination systems.

Usefulness & Problems

Why this is useful

SIBR-Cas is useful for bacterial genome engineering because it provides inducible and reportedly tight regulation of CRISPR-Cas counterselection. The cited study positions it as a simple and widely applicable approach for non-model bacteria, including hosts with poor homologous recombination systems.

Source:

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.

Source:

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas

Problem solved

This system addresses the problem that immediate CRISPR-Cas counterselection can prevent recovery of edited cells before recombination occurs. It is specifically presented as enabling gene knockout in bacteria with poor homologous recombination capacity without exogenous recombinases.

Source:

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.

Problem links

Need better screening or enrichment leverage

Derived

SIBR-Cas, termed Self-splicing Intron-Based Riboswitch-Cas, is a multi-component CRISPR genome engineering system for bacteria that provides inducible control over CRISPR-Cas counterselection. It is reported to delay counterselection to allow editing events to occur and has been applied to gene knockout in bacteria with poor homologous recombination systems.

Need conditional recombination or state switching

Derived

SIBR-Cas, termed Self-splicing Intron-Based Riboswitch-Cas, is a multi-component CRISPR genome engineering system for bacteria that provides inducible control over CRISPR-Cas counterselection. It is reported to delay counterselection to allow editing events to occur and has been applied to gene knockout in bacteria with poor homologous recombination systems.

Need controllable genome or transcript editing

Derived

SIBR-Cas, termed Self-splicing Intron-Based Riboswitch-Cas, is a multi-component CRISPR genome engineering system for bacteria that provides inducible control over CRISPR-Cas counterselection. It is reported to delay counterselection to allow editing events to occur and has been applied to gene knockout in bacteria with poor homologous recombination systems.

Taxonomy & Function

Primary hierarchy

Mechanism Branch

Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.

Target processes

editingrecombinationselection

Implementation Constraints

cofactor dependency: compatible with endogenous cofactorencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenoperating role: regulatorswitch architecture: multi component

The available evidence indicates that SIBR-Cas is a multi-component system built around a self-splicing intron-based riboswitch for inducible control of CRISPR-Cas counterselection. It was used for bacterial gene knockout without exogenous recombinases, but the supplied evidence does not specify the Cas protein, intron architecture, inducer, construct design, or delivery method.

The supplied evidence does not provide quantitative editing efficiencies, leakiness measurements, inducer identity, or direct comparisons against specific alternative systems. Validation is described from a single 2021 source, so independent replication and breadth across diverse bacterial taxa are not established here.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Observations

successBacteriaapplication demo

Inferred from claim c4 during normalization. Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems. Derived from claim c4. Quoted text: Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.

Source:

number of bacteria3
successBacteriaapplication demo

Inferred from claim c4 during normalization. Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems. Derived from claim c4. Quoted text: Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.

Source:

number of bacteria3
successBacteriaapplication demo

Inferred from claim c4 during normalization. Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems. Derived from claim c4. Quoted text: Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.

Source:

number of bacteria3
successBacteriaapplication demo

Inferred from claim c4 during normalization. Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems. Derived from claim c4. Quoted text: Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.

Source:

number of bacteria3
successBacteriaapplication demo

Inferred from claim c4 during normalization. Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems. Derived from claim c4. Quoted text: Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.

Source:

number of bacteria3
successBacteriaapplication demo

Inferred from claim c4 during normalization. Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems. Derived from claim c4. Quoted text: Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.

Source:

number of bacteria3
successBacteriaapplication demo

Inferred from claim c4 during normalization. Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems. Derived from claim c4. Quoted text: Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.

Source:

number of bacteria3

Supporting Sources

Ranked Claims

Claim 1application resultsupports2021Source 1needs review

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Claim 2application resultsupports2021Source 1needs review

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Claim 3application resultsupports2021Source 1needs review

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Claim 4application resultsupports2021Source 1needs review

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Claim 5application resultsupports2021Source 1needs review

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Claim 6application resultsupports2021Source 1needs review

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Claim 7application resultsupports2021Source 1needs review

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Claim 8application resultsupports2021Source 1needs review

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Claim 9application resultsupports2021Source 1needs review

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Claim 10application resultsupports2021Source 1needs review

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Claim 11application resultsupports2021Source 1needs review

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Claim 12application resultsupports2021Source 1needs review

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Claim 13application resultsupports2021Source 1needs review

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Claim 14application resultsupports2021Source 1needs review

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Claim 15application resultsupports2021Source 1needs review

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Claim 16application resultsupports2021Source 1needs review

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Claim 17application resultsupports2021Source 1needs review

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.
number of bacteria 3
Claim 18comparative advantagesupports2021Source 1needs review

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Claim 19comparative advantagesupports2021Source 1needs review

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Claim 20comparative advantagesupports2021Source 1needs review

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Claim 21comparative advantagesupports2021Source 1needs review

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Claim 22comparative advantagesupports2021Source 1needs review

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Claim 23comparative advantagesupports2021Source 1needs review

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Claim 24comparative advantagesupports2021Source 1needs review

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Claim 25comparative advantagesupports2021Source 1needs review

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Claim 26comparative advantagesupports2021Source 1needs review

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Claim 27comparative advantagesupports2021Source 1needs review

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Claim 28comparative advantagesupports2021Source 1needs review

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Claim 29comparative advantagesupports2021Source 1needs review

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Claim 30comparative advantagesupports2021Source 1needs review

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Claim 31comparative advantagesupports2021Source 1needs review

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Claim 32comparative advantagesupports2021Source 1needs review

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Claim 33comparative advantagesupports2021Source 1needs review

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Claim 34comparative advantagesupports2021Source 1needs review

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.
Claim 35mechanismsupports2021Source 1needs review

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Claim 36mechanismsupports2021Source 1needs review

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Claim 37mechanismsupports2021Source 1needs review

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Claim 38mechanismsupports2021Source 1needs review

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Claim 39mechanismsupports2021Source 1needs review

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Claim 40mechanismsupports2021Source 1needs review

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Claim 41mechanismsupports2021Source 1needs review

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Claim 42mechanismsupports2021Source 1needs review

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Claim 43mechanismsupports2021Source 1needs review

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Claim 44mechanismsupports2021Source 1needs review

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Claim 45mechanismsupports2021Source 1needs review

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Claim 46mechanismsupports2021Source 1needs review

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Claim 47mechanismsupports2021Source 1needs review

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Claim 48mechanismsupports2021Source 1needs review

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Claim 49mechanismsupports2021Source 1needs review

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Claim 50mechanismsupports2021Source 1needs review

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Claim 51mechanismsupports2021Source 1needs review

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.
Claim 52mechanismsupports2021Source 1needs review

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection
Claim 53mechanismsupports2021Source 1needs review

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection
Claim 54mechanismsupports2021Source 1needs review

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection
Claim 55mechanismsupports2021Source 1needs review

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection
Claim 56mechanismsupports2021Source 1needs review

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection
Claim 57mechanismsupports2021Source 1needs review

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection
Claim 58mechanismsupports2021Source 1needs review

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection
Claim 59mechanismsupports2021Source 1needs review

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection
Claim 60mechanismsupports2021Source 1needs review

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection
Claim 61mechanismsupports2021Source 1needs review

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection
Claim 62mechanismsupports2021Source 1needs review

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection
Claim 63mechanismsupports2021Source 1needs review

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection
Claim 64mechanismsupports2021Source 1needs review

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection
Claim 65mechanismsupports2021Source 1needs review

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection
Claim 66mechanismsupports2021Source 1needs review

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection
Claim 67mechanismsupports2021Source 1needs review

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection
Claim 68mechanismsupports2021Source 1needs review

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection
Claim 69proposed applicationsupports2021Source 1needs review

SIBR is proposed as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria.

we propose that SIBR can have a wider application as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria
Claim 70proposed applicationsupports2021Source 1needs review

SIBR is proposed as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria.

we propose that SIBR can have a wider application as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria
Claim 71proposed applicationsupports2021Source 1needs review

SIBR is proposed as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria.

we propose that SIBR can have a wider application as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria
Claim 72proposed applicationsupports2021Source 1needs review

SIBR is proposed as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria.

we propose that SIBR can have a wider application as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria
Claim 73proposed applicationsupports2021Source 1needs review

SIBR is proposed as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria.

we propose that SIBR can have a wider application as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria
Claim 74proposed applicationsupports2021Source 1needs review

SIBR is proposed as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria.

we propose that SIBR can have a wider application as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria
Claim 75proposed applicationsupports2021Source 1needs review

SIBR is proposed as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria.

we propose that SIBR can have a wider application as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria
Claim 76proposed applicationsupports2021Source 1needs review

SIBR is proposed as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria.

we propose that SIBR can have a wider application as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria
Claim 77proposed applicationsupports2021Source 1needs review

SIBR is proposed as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria.

we propose that SIBR can have a wider application as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria
Claim 78proposed applicationsupports2021Source 1needs review

SIBR is proposed as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria.

we propose that SIBR can have a wider application as a universal gene expression and gene regulation control mechanism for any gene or RNA of interest in bacteria
Claim 79tool descriptionsupports2021Source 1needs review

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Claim 80tool descriptionsupports2021Source 1needs review

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Claim 81tool descriptionsupports2021Source 1needs review

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Claim 82tool descriptionsupports2021Source 1needs review

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Claim 83tool descriptionsupports2021Source 1needs review

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Claim 84tool descriptionsupports2021Source 1needs review

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Claim 85tool descriptionsupports2021Source 1needs review

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Claim 86tool descriptionsupports2021Source 1needs review

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Claim 87tool descriptionsupports2021Source 1needs review

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Claim 88tool descriptionsupports2021Source 1needs review

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Claim 89tool descriptionsupports2021Source 1needs review

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Claim 90tool descriptionsupports2021Source 1needs review

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Claim 91tool descriptionsupports2021Source 1needs review

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Claim 92tool descriptionsupports2021Source 1needs review

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Claim 93tool descriptionsupports2021Source 1needs review

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Claim 94tool descriptionsupports2021Source 1needs review

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas
Claim 95tool descriptionsupports2021Source 1needs review

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas

Approval Evidence

1 source5 linked approval claimsfirst-pass slug sibr-cas
we termed SIBR-Cas (Self-splicing Intron-Based Riboswitch-Cas)

Source:

application resultsupports

Without exogenous recombinases, SIBR-Cas was successfully applied to knock out several genes in three bacteria with poor homologous recombination systems.

Without the use of exogenous recombinases, SIBR-Cas was successfully applied to knock-out several genes in three bacteria with poor homologous recombination systems.

Source:

comparative advantagesupports

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated, and widely applicable for most non-model bacteria.

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.

Source:

mechanismsupports

Delaying CRISPR-Cas counterselection grants more time for the editing event to occur.

This control delays CRISPR-Cas counterselection, granting more time for the editing event (e.g., by homologous recombination) to occur.

Source:

mechanismsupports

SIBR-Cas provides universal and inducible control over CRISPR-Cas counterselection.

allows for universal and inducible control over CRISPR-Cas counterselection

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tool descriptionsupports

SIBR-Cas is a simple and universal genome engineering tool for bacteria.

developing a simple and universal genome engineering tool for bacteria which we termed SIBR-Cas

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Comparisons

Source-backed strengths

The reported strengths are simplicity, tight regulation, and broad applicability across most non-model bacteria. In the cited work, SIBR-Cas was successfully used without exogenous recombinases to knock out several genes in three bacterial species with poor homologous recombination systems.

Source:

Compared to other genome engineering tools, SIBR-Cas is simple, tightly regulated and widely applicable for most (non-model) bacteria.

Compared with CRISPR/Cas9 system

SIBR-Cas and CRISPR/Cas9 system address a similar problem space because they share editing, recombination, selection.

Shared frame: same top-level item type; shared target processes: editing, recombination, selection

Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.

Compared with CRISPR/Cas system

SIBR-Cas and CRISPR/Cas system address a similar problem space because they share editing, recombination, selection.

Shared frame: same top-level item type; shared target processes: editing, recombination, selection

Compared with high throughput screening

SIBR-Cas and high throughput screening address a similar problem space because they share editing, recombination, selection.

Shared frame: shared target processes: editing, recombination, selection

Relative tradeoffs: looks easier to implement in practice.

Ranked Citations

  1. 1.
    StructuralSource 12021Claim 1Claim 17Claim 16

    Extracted from this source document.